Abstract: Provided herein are systems and methods for processing and analyzing nucleic acids and other biomolecules. Methods may include processing nucleic acid molecules in an emulsion of droplets. Methods of analyzing nucleic acid molecules may include coupling nucleic acids to a bead or other support. Methods may include analysis of nucleic acid molecules using a redox mediator. In some cases, analysis of the nucleic acid molecule includes determining a nucleotide sequence of the nucleic acid molecule.

Abstract: Provided herein are systems and methods for processing and analyzing nucleic acids and other biomolecules. Methods may include processing nucleic acid molecules in an emulsion of droplets. Methods of analyzing nucleic acid molecules may include coupling nucleic acids to a bead or other support. Methods may include analysis of nucleic acid molecules using a redox mediator. In some cases, analysis of the nucleic acid molecule includes determining a nucleotide sequence of the nucleic acid molecule.

Abstract: Provided herein are devices and methods suitable for sequencing, detecting, amplifying, analyzing, and performing sample preparation procedures for nucleic acids and other molecules. In some cases, the devices and methods provided herein are used for computation.

Abstract: Provided herein are devices and methods suitable for sequencing, detecting, amplifying, analyzing, and performing sample preparation procedures for nucleic acids and other molecules. In some cases, the devices and methods provided herein are used for computation.

Abstract: Provided herein are systems and methods for processing and analyzing nucleic acids and other biomolecules. Methods may include processing nucleic acid molecules in an emulsion of droplets. Methods of analyzing nucleic acid molecules may include coupling nucleic acids to a bead or other support. Methods may include analysis of nucleic acid molecules using a redox mediator. In some cases, analysis of the nucleic acid molecule includes determining a nucleotide sequence of the nucleic acid molecule.

Abstract: Microfluidic devices have been developed to perform a range of high-throughput biochemical and cell-based assays over recent years. A design for a microfluidic device has been developed that resembles a microtiter plate, by placing the test chambers (each test chamber contains cells) and top-loading drug inlets (at least one per test chamber) in a grid according to the ANSI/SBS standards, yet offers the miniaturization and fluid handling advantages of microfluidics. This ensures that the device design is compatible with fluid handling and imaging equipment already in use for drug screening. A range of topologies have been determined that allow placement of various elements of this microfluidic network within the grid alignment constraints. A resistance equalization methodology has also been developed to reduce variability across assays run in different chambers of the microfluidic device.

Abstract: Provided herein are devices and methods suitable for sequencing, detecting, amplifying, analyzing, and performing sample preparation procedures for nucleic acids and other molecules. In some cases, the devices and methods provided herein are used for computation.

Abstract: Microfluidic chips have been developed to perform a range of high-throughput biochemical and cell-based assays over recent years. We have formulated an innovative design for a microfluidic chip that resembles a microtiter plate by placing the test chambers (each test chamber contains cells) and top-loading drug inlets (one per test chamber) in a grid according to the ANSI/SBS standards, yet offers the miniaturization and fluid handling advantages of microfluidics. This ensures that our chip design is compatible with fluid handling and imaging equipment already in use for drug screening. We have determined a range of topologies that allow placement of various elements of this microfluidic network within the grid alignment constraints. We also developed a resistance equalization methodology to reduce variability across assays run in different chambers of the microfluidic chip. Additionally, it offers orders of magnitude miniaturization over multiwell plates, and potentially more reliable fluid handling.

Abstract: The ability to form and maintain gradients is essential for the study of response of cells to various stimuli. The invention includes devices and methods for the high-throughput, reproducible formation of gradients for the study of living cells. The invention includes microfluidics device with a lest chamber having a depth flanked by flow-through channels having a deeper depth. Flow of two different fluids through the flow-through channels results in the creation of a gradient by diffusion across the test chamber having essentially no flow.

Abstract: The ability to form and maintain gradients is essential for the study of response of cells to various stimuli. The invention includes devices and methods for the high-throughput, reproducible formation of gradients for the study of living cells. The invention includes microfluidics device with a test chamber having a depth flanked by flow-through channels having a deeper depth. Flow of two different fluids through the flow-through channels results in the creation of a gradient by diffusion across the test chamber having essentially no flow.

Abstract: The ability to form and maintain gradients is essential for the study of response of cells to various stimuli. The invention includes devices and methods for the high-throughput, reproducible formation of gradients for the study of living cells. The invention includes microfluidics device with a test chamber having a depth flanked by flow-through channels having a deeper depth. Flow of two different fluids through the flow-through channels results in the creation of a gradient by diffusion across the test chamber having essentially no flow.