Abstract: Isoandrographolide-19-propionate for treatment of non-alcoholic fatty liver disease (NALFD) relates to the compound isoandrographolide-19-propionate (IAN-19P), having the structural formula: and pharmaceutically acceptable salts thereof. The compound is synthesized by reaction of isoandrographolide with anhydrous propionic acid in the presence of N,N?-dicyclohexylcarbodiimide and 4-dimethylaminopyridine. In vitro testing shows that isoandrographolide-19-propionate has lower toxicity than andrographolide, but has significantly high activity at a concentration of 50 ?M. IAN-19P produced normal triglyceride levels and significantly lowered lipoperoxide formation in palmitate-oleate induced steatotic HepG2 cells, and also lowered ALT leakage from the treated cells.

Abstract: The process for obtaining a naphthoquinone derivative from Streptomyces sp. includes providing a seed inoculum of a strain of Streptomyces sp.; culturing the Streptomyces sp. in a culture nutrient medium; centrifuging the culture nutrient medium to provide a supernatant and a biomass precipitate; admixing a water immiscible solvent to the supernatant to produce a water immiscible solvent extract layer and a water layer; and isolating the antimicrobial and cytotoxic compound from the water immiscible solvent extract layer by performing silica gel chromatography.