Abstract: A cell culture container includes a culture membrane held between first and second containers each including: a first surface which includes a holding portion for holding the culture membrane; a second surface which is opposite the first surface; a first cell and a second cell which are adjacent to each other in a second direction orthogonal to a first direction from the first surface toward the second surface; a partition wall which separates the first cell and the second cell and extends from the first surface toward the second surface; a communication portion which is formed between the partition wall and the second surface and causes the first cell and the second cell to communicate with each other; and a third surface which is opposite the partition wall in the second direction, defines the second cell together with the second surface and has an insertion opening formed.

Abstract: A cell culture apparatus includes: a cylindrical member that includes a body cylindrical portion and a culture membrane arranged at an opening end portion of the body cylindrical portion; and a cylindrical covering member that is fitted to the body cylindrical portion and includes (i) a first cylindrical portion which covers an outer circumferential surface of the body cylindrical portion when fitted to the cylindrical member and (ii) a second cylindrical portion which is adjacent to the first cylindrical portion in an axial direction of the covering member and forms a container with the culture membrane serving as a bottom surface when fitted to the cylindrical member, and the first cylindrical portion includes a first part in which a length in the axial direction is a first length and a second part in which the length is a second length shorter than the first length.

Abstract: A cell culture method includes: a preparation step of preparing a cell culture membrane that includes (i) a membrane body formed of a thermosetting resin and including a first surface and a second surface opposite the first surface and (ii) a plurality of through pores formed in the membrane body and penetrating from the first surface to the second surface; and a culture step of seeding and culturing cells on each of the first surface and the second surface of the cell culture membrane prepared, in the through pores, a first average pore diameter in the first surface is smaller than a second average pore diameter in the second surface and the pore density of the through pores is equal to or less than 2.0×105 pores/cm2.