Abstract: Provided is a cryopreservation device which is small-sized and light-weight and has a low price, and also which is easy to get a cryocane in and out of. The cryopreservation device is configured to include first rotation lid 13 rotatably mounted on upper opening of container body 11, second rotation lid 14 rotatably provided on circular opening provided on the first rotation lid, through-hole for getting a cryocane in and out of provided on the second rotation lid, tubular body 15 provided in a protruding manner upward from the second rotation lid, lifting device 16 arranged inside the tubular body, cane gripper 17 provided on the lifting device, and door 18 provided on the tubular body, wherein any of cryocanes held at a center portion to the periphery of the container body on the cryocane holder can be gripped by rotating the first rotation lid with respect to the upper opening and by rotating the second rotation lid with respect to the first rotation lid to adjust the position of the through-hole.