Patents by Inventor Scot Wolfe

Scot Wolfe has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Targeting BCL11A distal regulatory elements with a CAS9-CAS9 fusion for fetal hemoglobin reinduction
    Patent number: 12098363
    Abstract: Provided herein are methods and compositions for increasing fetal hemoglobin levels in a cell by disrupting BCL11A expression at the genomic level. Also provided herein are methods and compositions relating to the treatment of hemoglobinopathies by reinduction of fetal hemoglobin levels.
    Type: Grant
    Filed: July 20, 2018
    Date of Patent: September 24, 2024
    Assignees: THE CHILDREN'S MEDICAL CENTER CORPORATION, UNIVERSITY OF MASSACHUSETTS
    Inventors: Daniel E. Bauer, Scot Wolfe, Mehmet Fatih Bolukbasi, Benjamin Roscoe, Pengpeng Liu, Kevin Luk, Yuxuan Wu, Jing Zeng
  • PRIME EDITOR SYSTEM FOR IN VIVO GENOME EDITING
    Publication number: 20230374476
    Abstract: The present applications discloses an NLS-optimized SpCas9-based prime editor that improves genome editing efficiency exemplified by endogenous loci in cultured cell lines. Using this genome modification system, tumor formation can be initiated through somatic cell editing in the adult mouse. Furthermore, a dual adeno-associated vims (AAVs) is utilized for the delivery of a split-intein prime editor for correction of in vivo pathogenic mutations.
    Type: Application
    Filed: February 4, 2022
    Publication date: November 23, 2023
    Inventors: Shun-Qing Liang, Pengpeng Liu, Wen Xue, Scot Wolfe
  • MODIFIED GUIDE RNAS FOR CRISPR GENOME EDITING
    Publication number: 20210388348
    Abstract: Chemically modified crRNAs and tracrRNAs are provided. crRNAs and tracrRNAs with 5? and/or 3? conjugated moieties are provided. crRNAs and tracrRNAs with modifications in the repeat region of the crRNA or the anti-repeat region of the tracrRNA are provided. Methods of using the crRNAs and tracrRNAs for genome editing with a CRISPR nuclease and kits for performing the same are also provided.
    Type: Application
    Filed: May 12, 2021
    Publication date: December 16, 2021
    Inventors: Erik Joseph Sontheimer, Anastasia Khvorova, Jonathan Kenneth Watts, Nadia Amrani, Zexiang Chen, Matthew Hassler, Dimas Echeverria Moreno, Julia Frances Alterman, Scot Wolfe, Ken Yamada, Gitali Devi, Han Zhang
  • Zinc finger-based drug-dependent gene regulation system
    Publication number: 20060288437
    Abstract: The invention is based on the discovery that one can create a drug-regulated gene regulation system by creating a fusion polypeptide or chimera that includes three portions: a zinc finger (ZF) protein that recognizes and binds to a nucleic acid recognition sequence in the vicinity of a promoter of the target gene, (ii) a dimerization domain of a tetracycline repressor, which contains a drug-dependent switch, and (iii) an accessory domain to alter the expression of the target gene (such as an activation or repression domain). This chimera is administered to, or expressed in, a cell harboring the target gene to be regulated.
    Type: Application
    Filed: August 18, 2006
    Publication date: December 21, 2006
    Inventor: Scot Wolfe
  • Bacterial counterselectable hybrid systems
    Publication number: 20050287550
    Abstract: The present application relates to the hybrid selection methods in prokaryotes using counterselectable reporter genes.
    Type: Application
    Filed: February 2, 2005
    Publication date: December 29, 2005
    Inventors: Scot Wolfe, Xiangdong Meng, Jay Joung
  • Zinc finger-based drug-dependent gene regulation system
    Publication number: 20050042732
    Abstract: The invention is based on the discovery that one can create a drug-regulated gene regulation system by creating a fusion polypeptide or chimera that includes three portions: a zinc finger (ZF) protein that recognizes and binds to a nucleic acid recognition sequence in the vicinity of a promoter of the target gene, (ii) a dimerization domain of a tetrac repressor, which contains a drug-dependent switch, and (iii) an accessory domain to alter the expression of the target gene (such as an activation or repression domain). This chimera is administered to, or expressed in, a cell harboring the target gene to be regulated.
    Type: Application
    Filed: December 9, 2002
    Publication date: February 24, 2005
    Inventor: Scot Wolfe