Abstract: Embodiments of the present invention are directed to improved methods and devices for analyzing a cell, aggregated cells, or a solid tumor. Such methods and devices are, for example, useful in the field of pathology and can provide improved cell processing and analytical results.

Abstract: Embodiments of the present invention are directed to improved methods and devices for analyzing a cell, aggregated cells, or a solid tumor. Such methods and devices are, for example, useful in the field of pathology and can provide improved cell processing and analytical results.

Abstract: A non-metallic fence system has a singular snap lock mechanism for permitting the easy construction of the fence.

Abstract: Devices and methods for delivery or retrieval of a distal protection device. A distal protection assembly may comprise an outer sheath, an inner shaft disposed within a lumen of the outer sheath, a distal protection device disposed at a distal end of the inner shaft; and a manifold coupled to a proximal end of the outer sheath that is adapted for moving the outer sheath relative to the inner shaft.

Abstract: Disclosed is a manifold including a housing having multiple ports and multiple bores networking the ports. An actuatable valve, such as a slidable valve, including one or more bores may be positioned in the housing such that the valve is actuatable between a first position and a second position. Actuation of the valve may selectively establish a desired fluid pathway through the manifold. One or more seals may be interposed between the valve and the housing. The seal includes at least a first sealing element, a second sealing element, and a land extending between the sealing elements. The sealing elements are configured to provide a fluid tight seal between the housing and the valve when the valve is in either the first or second position, and the land is configured to provide a fluid tight seal between the housing and the valve when the valve is in an intermediate position.

Abstract: Embodiments of the present invention are directed to improved methods and devices for analyzing a cell, aggregated cells, or a solid tumor. Such methods and devices are, for example, useful in the field of pathology and can provide improved cell processing and analytical results.

Abstract: Devices and methods for delivery or retrieval of a distal protection device. A distal protection assembly may comprise an outer sheath, an inner shaft disposed within a lumen of the outer sheath, a distal protection device disposed at a distal end of the inner shaft; and a manifold coupled to a proximal end of the outer sheath that is adapted for moving the outer sheath relative to the inner shaft.

Abstract: The present invention relates generally to the field of embolic protection and, more particularly, to systems for locking a shaft relative to a hub assembly. In addition, a method for securing a shaft relative to a hub assembly is disclosed.

Abstract: A peptide or protein microassay method and apparatus in which a wide variety of chromogenic or fluorogenic peptide or protein substrates of interest are individually suspended or dissolved in a hydrophilic carrier, with aliquots of each substrate being deposited in an array or microarray of reaction loci, or “dots.” Each dot, therefore, provides an individual reaction vessel containing the peptide or protein of interest, to which a biological sample may be applied for assay purposes. The sample is applied to the array or microarray of dots by one of a variety of focused sample application techniques, including aerosolizing or misting of the sample, or target application of the sample, onto each dot without creating fluid channels between the dots which would cause cross-contamination. In additional aspects, the present invention provides methods of transferring samples from an electrophoretic gel to a target plate for subsequent MALDI MS analysis.

Abstract: Compositions and methods of using compositions with a nuclear targeting peptide containing a nonclassical nuclear localization signal to deliver selected molecules to the nucleus of eukaryotic cells are provided. The compositions are particularly useful in gene transfer methods.

Abstract: The instant invention is drawn to methods useful for the treatment or the prevention of a viral infection. The methods include administering at least one compound that is an inhibitor of cathepsin L to an individual. The methods are particularly useful in individuals infected with, or at risk of infection with, SARS virus or Ebola virus. The invention also includes methods of identifying potential therapeutics for use in the methods of treatment or prevention of a viral infection.

Abstract: A number of devices are illustrated for providing a manifold that can simply and selectively couple a number of different therapeutic and diagnostic elements to a catheter or other medical device. Some examples include devices having sliding actuators, rotary actuators, button actuators, or combinations thereof, for easily changing the valve scheme and fluid pathways within a manifold.

Abstract: Photolytic cross-linkable polymers comprises three domains, a cationic domain, a cross-linkable domain and a photolabile domain. The photolytic cross-linkable polymers according to the current invention are useful in a method to complex and compact DNA and RNA for delivery to a living cell, wherein the DNA or RNA is released by photolytic degradation of a cross-linked polymer, which encapsulates the DNA or RNA in a nanoparticle.

Abstract: A fluid control apparatus is disclosed for controlling fluid flow between a catheter, an injector, a saline supply, a waste dump and a contrast supply. The apparatus includes a valve stem movable within a cavity disposed in a valve body. The valve stem is movable to a plurality of positions including a contrast position where the valve stem provides communication between the injector and a contrast supply, a saline/waste position where the valve stem provides communication between the injector and a saline/waste line and an injection position where the valve stem provides communication between the injector and an outlet port connected to the catheter.

Abstract: Compositions and methods of using compositions with a nuclear targeting peptide containing a nonclassical nuclear localization signal to deliver selected molecules to the nucleus of eukaryotic cells are provided. The compositions are particularly useful in gene transfer methods.

Abstract: A fluid control valve is disclosed which, with a single valve, can manipulate flow between a saline supply, a contrast supply, a waste dump and a catheter. The control valve includes three primary positions including a contrast position where the valve provides communication between an injector and a contrast supply while isolating the saline supply and catheter. The valve also can be moved to a saline/waste position where the valve provides communication between the injector and the saline supply and/or the waste dump while isolating the contrast supply and catheter. The valve also can be moved to an injection position where the valve provides communication between the injector and the catheter. A single valve provides all three functions. The valve also may provide communication between a pressure transducer and catheter during the saline loading, waste dumping and contrast loading functions. The valve also provides protection or isolation of the pressure transducer during injection procedures.

Abstract: A peptide or protein microassay method and apparatus in which a wide variety of chromogenic or fluorogenic peptide or protein substrates of interest are individually suspended or dissolved in a hydrophilic carrier, with aliquots of each substrate being deposited in an array or microarray of reaction loci, or “dots.” Each dot, therefore, provides an individual reaction vessel containing the peptide or protein of interest, to which a biological sample may be applied for assay purposes. The sample is applied to the array or microarray of dots by one of a variety of focused sample application techniques, including aerosolizing or misting of the sample, or target application of the sample, onto each dot without creating fluid channels between the dots which would cause cross-contamination. In additional aspects, the present invention provides methods of transferring samples from an electrophoretic gel to a target plate for subsequent MALDI MS analysis.

Abstract: The invention relates to compositions and methods for a one-step synthetic technique for making cationic steroid or cationic drug molecules for use as delivery vehicles. The invention further relates to methods for using cationic steroid molecules in lipofection or transfection, delivery of drugs, and for treatment of inflammation and other diseases and disorders. The invention also relates to cationic steroid prodrugs and cationic prodrugs and to methods of modifying drugs.

Abstract: A fluid control apparatus is disclosed for controlling fluid flow between a catheter, an injector, a saline supply, a waste dump and a contrast supply. The apparatus includes a valve stem movable within a cavity disposed in a valve body. The valve stem is movable to a plurality of positions including a contrast position where the valve stem provides communication between the injector and a contrast supply, a saline/waste position where the valve stem provides communication between the injector and a saline/waste line and an injection position where the valve stem provides communication between the injector and an outlet port connected to the catheter.

Abstract: A number of devices are illustrated for providing a manifold that can simply and selectively couple a number of different therapeutic and diagnostic elements to a catheter or other medical device. Some examples include devices having sliding actuators, rotary actuators, button actuators, or combinations thereof, for easily changing the valve scheme and fluid pathways within a manifold.