Abstract: Provided herein is a method for detecting the presence of clade variants in the COVID-19 virus in a human sample and/or an environmental sample. Samples are processed to obtain total RNA. The RNA is used as a template in a combined reverse transcription and amplification reaction to obtain fluorescent COVID-19 virus amplicons. These amplicons are hybridized on a microarray with nucleic acid probes having sequences that discriminate among the various clade variants. The microarray is imaged to detect the clade variant. Also provided is a method of distinguishing each clade variant from others by generating an intensity distribution profile from the image, which is unique to each of the clade variants.

Abstract: A speed select shift mechanism for a belt drive assembly to be installed on a shaft capable of shifting between high, low and neutral speeds is disclosed. The shift mechanism includes a shift collar that is enclosed within an enclosure to prevent debris from contacting the inner operations of the shift mechanism and thereby reduce wear and tear of the shift mechanism along with down time for maintenance of the device. The shift mechanism includes a shaft, a first pulley, a shift collar, a second pulley and a shifter. The shift collar further includes dowels that cooperatively engage corresponding apertures on the pulleys.

Abstract: A speed select shift mechanism for a belt drive assembly to be installed on a shaft capable of shifting between high, low and neutral speeds is disclosed. The shift mechanism includes a shift collar that is enclosed within an enclosure to prevent debris from contacting the inner operations of the shift mechanism and thereby reduce wear and tear of the shift mechanism along with down time for maintenance of the device. The shift mechanism includes a shaft, a first pulley, a shift collar, a second pulley and a shifter. The shift collar further includes dowels that cooperatively engage corresponding apertures on the pulleys.

Abstract: Wheel assemblies that include decorative wheel covers which are bonded to wheels by sound dampening adhesive patterns that include primary adhesive patterns that are sufficient to secure the wheel covers to the wheels and an auxiliary adhesive patterns that include discrete portions that are provided in hollow portions defined by the primary adhesive patterns between the wheel covers and wheels. The discrete portions of the auxiliary adhesive patterns prevent the hollow portions from sounding hollow.

Abstract: A method of fabricating decorative wheel covers which involves forming decorative wheel covers that have sharp edges such as edges along brake vent windows or openings. The shape edges are dulled by subjecting the wheel covers to a plating process that plates both sides of the wheel covers. The plating process rounds off or dulls the shape edges of the wheel covers. The ability to dull the sharp edges of the wheel covers allows for the design and fabrication of metal wheel covers having profiles that push the limits on the formability of metals such as stainless steel. The plating method also dulls sharp edges on plastic wheel covers.

Abstract: Inserts that are configured to be placed in gaps provide between wheel covers and underlying wheel of wheel assemblies. The inserts are made from materials that are suitable rigid and strong so as to reinforce and protect the overlying portions of the wheel cover from impact damage such as denting in the case of metal wheel covers and cracking or breaking in the case of plastic wheel covers. The inserts further prevent otherwise hollow portions of the wheel assembly from sounding, feeling or seeming hollow.

Abstract: Inserts that are configured to be placed in gaps provide between wheel covers and underlying wheel of wheel assemblies. The inserts are made from materials that are suitable rigid and strong so as to reinforce and protect the overlying portions of the wheel cover from impact damage such as denting in the case of metal wheel covers and cracking or breaking in the case of plastic wheel covers. The inserts further prevent otherwise hollow portions of the wheel assembly from sounding, feeling or seeming hollow.

Abstract: Wheel assemblies that include decorative wheel covers which are bonded to wheels by sound dampening adhesive patterns that include primary adhesive patterns that are sufficient to secure the wheel covers to the wheels and an auxiliary adhesive patterns that include discrete portions that are provided in hollow portions defined by the primary adhesive patterns between the wheel covers and wheels. The discrete portions of the auxiliary adhesive patterns prevent the hollow portions from sounding hollow.

Abstract: Adhesive patterns are applied to one of an outboard side of a wheel and an inner surface of a decorative wheel cover and used to bond the decorative wheel cover to the wheel with a spaced therebetween that is partially filled with the cured adhesive. The decorative wheel cover and wheel each include aligned lug nut apertures and aligned decorative openings. The adhesive patterns are configured to allow ambient fluids to enter throughout the space between the decorative wheel cover and the wheel (and leave) which is not filled with the cured adhesive to prevent corrosion from occurring between the decorative wheel cover and wheel.

Abstract: Adhesive patterns are applied to one of an outboard side of a wheel and an inner surface of a decorative wheel cover and used to bond the decorative wheel cover to the wheel with a spaced therebetween that is partially filled with the cured adhesive. The decorative wheel cover and wheel each include aligned lug nut apertures and aligned decorative openings. The adhesive patterns are configured to allow ambient fluids to enter throughout the space between the decorative wheel cover and the wheel (and leave) which is not filled with the cured adhesive to prevent corrosion from occurring between the decorative wheel cover and wheel.

Abstract: A composite wheel assembly includes a wheel having an outboard surface and a wheel ornamentation disposed over the outboard surface. The wheel ornamentation has a plurality of vent openings and a plurality of lug stud holes. The composite wheel assembly also includes an adhesive disposed between the wheel ornamentation and the wheel around each of the vent openings. The composite wheel assembly further includes at least one seal comprising an o-ring disposed between the wheel ornamentation and the wheel about at least one of the lug stud holes to prevent contaminants from entering the assembly between the wheel ornamentation and the wheel.

Abstract: A method of making a wheel cover having a decorative outboard surface and an inboard surface for placement on a vehicle wheel includes the steps of forming a wheel cover substrate having a substrate contour providing the inboard substrate surface and with an outer substrate surface for bonding a skin thereto. The skin has a decoration applied to an inner skin surface for placement between the skin and the substrate. The skin has a decoration applied thereto. It is then cut to a desired shape complementing the substrate. It may, at that point, be deformed to also complement to contour of the substrate. The skin is bonded to the substrate by welding the materials to one another or by an adhesive.

Abstract: A wheel cover assembly includes a cover and a plurality of lug wells. The cover is manufactured from a first material that is capable of being decorated with paint and/or plating. The lug wells are fabricated from a second material. The second material is chosen for its high thermal resistive characteristics. The second material insulates the first material from the thermal energy generated by the wheel hub assembly and, in particular, the brake systems of the wheel hub assembly. Each of the lug wells includes a wall having an annular recess circumscribing the interior surface of the wall and an exterior ridge extending around the periphery of the wall. The annular recess and the exterior ridge prevent the first material from flowing therepast creating a barrier of the second material used to manufacture the lug wells to the lug nuts, the conductors of the thermal energy created by the brake systems. Protrusions and elongated holes aid in the bonding of the first material to the second material.

Abstract: A method has been developed for control of molecular weight and molecular weight dispersity during production of polyhydroxyalkanoates in genetically engineered organism by control of the level and time of expression of one or more PHA synthases in the organisms. The method was demonstrated by constructing a synthetic operon for PHA production in E. coli in which the level of PHA synthase activity could be tightly controlled by placement of the synthase behind an inducible promoter. Modulation of the total level of PHA synthase activity in the host cell by varying the concentration of the inducer, isopropyl .beta.-D-thiogalactoside (IPTG), was found to effect the molecular weight of the polymer produced in the cell. Specifically, high concentrations of synthase activity were found to yield polymers of low molecular weight while low concentrations of synthase activity yielded polymers of higher molecular weight.

Abstract: A component lead cutting apparatus for tape-fed radial lead electrical components is disclosed. The taped components are driven through the machine by a rotary index wheel, with the cutting station element adjacent the wheel periphery. Clamping and cutting die elements are placed at the cutting station, and are actuated by a pneumatic cylinder to clamp and cut the lead components. The apparatus includes adjustment elements readily accessible from the top of the machine to raise or lower the index wheel and thereby adjust for different lead cut lengths, and to position the wheel to accommodate different tape sprocket opening configurations. The machine has a compact, narrow footprint, so that the machine is space-efficient.