Abstract: Devices, systems, and methods for collecting samples for medical testing. A sample collection device includes a collection chamber coupled to a body portion. The collection chamber extends from a first collection chamber end to a second collection chamber end. The collection chamber includes an opening proximate the second collection chamber end. The opening is configured to receive a first volume of the biological sample. A projection extends into the collection chamber. The collection chamber is configured to translate relative to the projection between a first position and a second position, wherein translation of the collection chamber from the first position to the second position causes a second volume of the biological sample to be released from the collection chamber through the opening.

Abstract: The presently disclosed subject matter provides methods of isolating biomarker cells from a liquid sample, and related methods of capturing DNA or RNA released from biomarker cells.

Abstract: The presently disclosed subject matter provides methods of isolating biomarker cells from a liquid sample, and related methods of capturing DNA or RNA released from biomarker cells.

Abstract: The presently disclosed subject matter provides a fluid-tight flow system; a method of isolating biomarker cells from a liquid sample; a method of extracting cfDNA, ctDNA, or exosomes from plasma; a method of extracting cfDNA, ctDNA, or exosomes from blood; a method of capturing DNA or RNA released from biomarker cells; a method of amplifying a nucleotide sequence; a method of sequencing a nucleotide sequence; a method of detecting a chromosomal abnormality; and a method of analyzing a protein.