Abstract: In one aspect, a share data allocation (SDA) computing device is provided. The SDA computing device includes at least one processor in communication with a database. The at least one processor is configured to receive direct-share participant (DSP) request data indicating an amount each DSP is willing to invest and receive interest-based participant (IBP) request data indicating an amount each IBP is willing to invest. The at least one processor is also configured to apply a first mapping algorithm to the DSP request data and apply a second mapping algorithm to the IBP request data. The at least one processor is further configured to calculate a preliminary allocation of a total amount of assets among the DSPs and the IBPs according to results of an apportionment, and direct allocation of the total amount of assets among the DSPs and the IBPs according to results of a join operator.

Abstract: Methods and compositions are provided for reversibly localizing proteins to the exterior part of the cell surface. Compositions provided herein can include nucleic acids that encode polypeptides of interest and the ligand binding domain (LBD) of a nuclear hormone receptor. Medical applications are provided, including controlling the toxicity of CAR T cells.

Abstract: Compositions and methods for generating and utilizing spatial and temporal distributions of molecules are disclosed. The effect is based on the observation that certain proteins that serve a spatial and/or temporal function in their native environments can be expressed in different environments to achieve different spatial and/or temporal distributions. This observation showed that whole-cell oscillations, traveling waves, spirals, turbulent patterns, static patterns, and other patterns could be generated and observed. These spatial and temporal distributions were then coupled with functional moieties, which provided the functionality in the same spatial and temporal distributions. The model system for these observations is expression of MinD and MinE proteins in Eukaryotic cells.

Abstract: In one aspect, a share data allocation (SDA) computing device is provided. The SDA computing device includes at least one processor in communication with a database. The at least one processor is configured to receive direct-share participant (DSP) request data indicating an amount each DSP is willing to invest and receive interest-based participant (IBP) request data indicating an amount each IBP is willing to invest. The at least one processor is also configured to apply a first mapping algorithm to the DSP request data and apply a second mapping algorithm to the IBP request data. The at least one processor is further configured to calculate a preliminary allocation of a total amount of assets among the DSPs and the IBPs according to results of an apportionment, and direct allocation of the total amount of assets among the DSPs and the IBPs according to results of a join operator.

Abstract: In one aspect, a share data allocation (SDA) computing device is provided. The SDA computing device includes at least one processor in communication with a database. The at least one processor is configured to receive direct-share participant (DSP) request data indicating an amount each DSP is willing to invest and receive interest-based participant (IBP) request data indicating an amount each IBP is willing to invest. The at least one processor is also configured to apply a first mapping algorithm to the DSP request data and apply a second mapping algorithm to the IBP request data. The at least one processor is further configured to calculate a preliminary allocation of a total amount of assets among the DSPs and the IBPs according to results of an apportionment, and direct allocation of the total amount of assets among the DSPs and the IBPs according to results of a join operator.

Abstract: An event analysis (EA) computing device is described. The EA computing device includes at least one processor in communication with a database and a plurality of user computing devices. The at least one processor is configured to receive a user interaction data signal, store a first data extracted from the user interaction data signal in a first database, (i) transmit a notification data signal to one of the user computing devices, and (ii) generate and store a user trail record in a second database as a linked element in a user trail. The processor is also configured to retrieve the first data from the first database and use the first data to implement a primary functionality of the EA computing device, retrieve the user trail and use the user trail to generate a report, and transmit the report to one of the user computing devices.

Abstract: Methods and compositions are provided for reversibly localizing proteins to the exterior part of the cell surface. Compositions provided herein can include nucleic acids that encode polypeptides of interest and the ligand binding domain (LBD) of a nuclear hormone receptor. Medical applications are provided, including controlling the toxicity of CAR T cells.

Abstract: Methods and compositions are provided for reversibly localizing proteins to the exterior part of the cell surface. Compositions provided herein can include nucleic acids that encode polypeptides of interest and the ligand binding domain (LBD) of a nuclear hormone receptor. Medical applications are provided, including controlling the toxicity of CAR T cells.

Abstract: In one aspect, a share data allocation (SDA) computing device is provided. The SDA computing device includes at least one processor in communication with a database. The at least one processor is configured to receive direct-share participant (DSP) request data indicating an amount each DSP is willing to invest and receive interest-based participant (IBP) request data indicating an amount each IBP is willing to invest. The at least one processor is also configured to apply a first mapping algorithm to the DSP request data and apply a second mapping algorithm to the IBP request data. The at least one processor is further configured to calculate a preliminary allocation of a total amount of assets among the DSPs and the IBPs according to results of an apportionment, and direct allocation of the total amount of assets among the DSPs and the IBPs according to results of a join operator.

Abstract: An asset vector analysis (AVA) computing device retrieves, from a database, investor data relating to a plurality of individual investors and past investment activity of the plurality of investors. The device computes, for each individual investor, an investor score, and transmits a notification of a public offering of assets to at least some of the individual investors. The device receives, from the individual investors, a response indicating an amount of money the respective investor is willing to invest in the public offering. The device determines a total amount of assets available to the individual investors in the public offering. The device allocates a portion of the total amount of assets available to the individual investors based at least in part on the investor score of individual investors.

Abstract: Methods and compositions are provided for reversibly localizing proteins to the exterior part of the cell surface. Compositions provided herein can include nucleic acids that encode polypeptides of interest and the ligand binding domain (LBD) of a nuclear hormone receptor. Medical applications are provided, including controlling the toxicity of CAR T cells.