Abstract: A method and system for integrated and continuous viral filtration and biological product concentration, including an initial purification system coupled to a final purification system. The initial purification system includes a viral reduction filtration (VRF) skid, while the final purification system includes a single-pass tangential filtration-diafiltration (SPTFF-DF) skid.

Abstract: Methods for continuously inactivating virus during manufacture of a biological product are provided. The methods include steps of (1) combining (a) a composition including a biological product, and (b) a composition including a virus-inactivation reagent, to obtain (c) a treatment composition having a predetermined property for inactivation of a virus, (2) confirming that the treatment composition exhibits the predetermined property, (3) transferring the treatment composition to a treatment vessel that includes an inlet, an outlet, and a static mixer, the transferring occurring at the inlet, (4) incubating the treatment composition in the treatment vessel at a predetermined temperature while the treatment composition flows at a predetermined rate and contacts the static mixer, and (5) collecting the treatment composition from the treatment vessel at the outlet, wherein steps (1) to (5) are carried out continuously. Apparatuses and systems including such a treatment vessel are also provided.

Abstract: A capacitor and capacitor assemblies are provided, configured to prevent damage from shock and/or vibration. A capacitor assembly according to the invention comprises an anode plate having an anode plate wire extending from a surface of the anode plate. An anode wire holder is positioned around at least a portion of the anode plate wire. A wire separator comprising a channel is provided, at least a portion of the anode plate wire received within the channel. Methods of forming capacitors and capacitor assemblies are also provided.

Abstract: Methods for continuously inactivating virus during manufacture of a biological product are provided. The methods include steps of (1) combining (a) a composition including a biological product, and (b) a composition including a virus-inactivation reagent, to obtain (c) a treatment composition having a predetermined property for inactivation of a virus, (2) confirming that the treatment composition exhibits the predetermined property, (3) transferring the treatment composition to a treatment vessel that includes an inlet, an outlet, and a static mixer, the transferring occurring at the inlet, (4) incubating the treatment composition in the treatment vessel at a predetermined temperature while the treatment composition flows at a predetermined rate and contacts the static mixer, and (5) collecting the treatment composition from the treatment vessel at the outlet, wherein steps (1) to (5) are carried out continuously. Apparatuses and systems including such a treatment vessel are also provided.

Abstract: A device and method for in-line homogenizing a non-uniform feed stream is described herein, which includes a plug flow reactor (PFR), a bypass line, and a pump in a closed-circuit flow path that allows for rapid homogenization of the non-uniform feed stream.

Abstract: A capacitor and capacitor assemblies are provided, configured to prevent damage from shock and/or vibration. A capacitor assembly according to the invention comprises an anode plate having an anode plate wire extending from a surface of the anode plate. An anode wire holder is positioned around at least a portion of the anode plate wire. A wire separator comprising a channel is provided, at least a portion of the anode plate wire received within the channel. Methods of forming capacitors and capacitor assemblies are also provided.

Abstract: A weather station system is provided, comprising: a weather station having at least one sensor disposed at an intersection with a traffic cabinet; an interface card adapted for insertion in a detection rack of a traffic cabinet. The card has embedded firmware to receive data from the at least one sensor of the weather station and communicate the data to at least one remote computing device for processing with data of other weather stations. A weather data processing system and a method of retrieving localized weather data are also provided.

Abstract: Integrated systems and methods for the manufacturing of recombinant proteins in mammalian cells are provided. The systems and methods include a single-use bioreactor (SUB), a tangential flow filtration system, a column chromatography skid, a tankless hold, a virus inactivation plug flow reactor, and pumps.

Abstract: A viral inactivation device including at least one continuous viral inactivation reactor having an inlet, an outlet, and a tubular flow path. The tubular flow path includes a set of alternating turns that form a serpentine pattern between the inlet and the outlet.

Abstract: The present invention provides improved bioprocessing systems and methods for cell culture using the improved bioreactors, e.g., batch-fed or perfusion bioreactor cell culture systems for production of monoclonal or bi-specific antibodies, which are modified to include one or more membrane gas transfer modules in place of a sparger- or microsparger-based aeration systems to better regulate the levels of critical gases in a bioreactor cell culture, e.g., the dissolved levels of O2 and CO2, even at high cell densities, without subjecting the cells to bubble-burst associated cell death.

Abstract: Methods for continuously inactivating virus during manufacture of a biological product are provided. The methods include steps of (1) combining (a) a composition including a biological product, and (b) a composition including a virus-inactivation reagent, to obtain (c) a treatment composition having a predetermined property for inactivation of a virus, (2) confirming that the treatment composition exhibits the predetermined property, (3) transferring the treatment composition to a treatment vessel that includes an inlet, an outlet, and a static mixer, the transferring occurring at the inlet, (4) incubating the treatment composition in the treatment vessel at a predetermined temperature while the treatment composition flows at a predetermined rate and contacts the static mixer, and (5) collecting the treatment composition from the treatment vessel at the outlet, wherein steps (1) to (5) are carried out continuously. Apparatuses and systems including such a treatment vessel are also provided.

Abstract: Methods for continuously inactivating virus during manufacture of a biological product are provided. The methods include steps of (1) combining (a) a composition including a biological product, and (b) a composition including a virus-inactivation reagent, to obtain (c) a treatment composition having a predetermined property for inactivation of a virus, (2) confirming that the treatment composition exhibits the predetermined property, (3) transferring the treatment composition to a treatment vessel that includes an inlet, an outlet, and a static mixer, the transferring occurring at the inlet, (4) incubating the treatment composition in the treatment vessel at a predetermined temperature while the treatment composition flows at a predetermined rate and contacts the static mixer, and (5) collecting the treatment composition from the treatment vessel at the outlet, wherein steps (1) to (5) are carried out continuously. Apparatuses and systems including such a treatment vessel are also provided.

Abstract: The subject technology relates to a cell retention device and method for use in a perfusion cell culture system where the cell retention device includes a hollow-fiber filter having an average pore size ranging from about 0.5 to about 20 ?m and having the ability to operate under perfusion cell culture conditions for up to 35 days without being clogged or losing its product sieving ability by more than 20%.

Abstract: Methods for continuously inactivating virus during manufacture of a biological product are provided. The methods include steps of (1) combining (a) a composition including a biological product, and (b) a composition including a virus-inactivation reagent, to obtain (c) a treatment composition having a predetermined property for inactivation of a virus, (2) confirming that the treatment composition exhibits the predetermined property, (3) transferring the treatment composition to a treatment vessel that includes an inlet, an outlet, and a static mixer, the transferring occurring at the inlet, (4) incubating the treatment composition in the treatment vessel at a predetermined temperature while the treatment composition flows at a predetermined rate and contacts the static mixer, and (5) collecting the treatment composition from the treatment vessel at the outlet, wherein steps (1) to (5) are carried out continuously. Apparatuses and systems including such a treatment vessel are also provided.

Abstract: Locking clips are provided for attaching a lamp assembly in a traffic signal housing. The clips use a fixed first portion and a movable second portion. The second portion locks to the first portion or to the housing itself and retains an edge of the lamp assembly, holding it in a window of the housing. Foldable/hinged interlocking clips are provided. Other alternatives include slidable clips that engage a fixed element on the housing (e.g. a fixed pin, or a trough in the housing wall), and rotatable clips that engage a fixed element on the housing (e.g. a fixed pin, or a trough in the housing wall). The clips may be part of a retaining system or a traffic signal. Optional lower holders may also be provided. The invention also includes a method for replacing a lamp assembly in a traffic signal housing with at least one locking clip.

Abstract: Locking clips are provided for attaching a lamp assembly in a traffic signal housing. The clips use a fixed first portion and a movable second portion. The second portion locks to the first portion or to the housing itself and retains an edge of the lamp assembly, holding it in a window of the housing. Foldable/hinged interlocking clips are provided. Other alternatives include slidable clips that engage a fixed element on the housing (e.g. a fixed pin, or a trough in the housing wall), and rotatable clips that engage a fixed element on the housing (e.g. a fixed pin, or a trough in the housing wall). The clips may be part of a retaining system or a traffic signal. Optional lower holders may also be provided. The invention also includes a method for replacing a lamp assembly in a traffic signal housing with at least one locking clip.

Abstract: A system provides for high level disinfection or sterilization of an endoscope having a first portion adapted for immersion and a second portion not adapted for immersion. The system includes a container having a first basin sized and adapted to receive the first portion of the endoscope for immersion, a separate and adjacent second basin sized and adapted to receive the second portion of the endoscope, a wall between the first basing and the second basin, and a trough in an upper portion of the wall between the first basin and second basin through which a tube connecting the first portion of the endoscope and the second portion of the endoscope may extend.

Abstract: A system provides for high level disinfection or sterilization of an endoscope having a first portion adapted for immersion and a second portion not adapted for immersion. The system includes a container having a first basin sized and adapted to receive the first portion of the endoscope for immersion, a separate and adjacent second basin sized and adapted to receive the second portion of the endoscope, a wall between the first basing and the second basin, and a trough in an upper portion of the wall between the first basin and second basin through which a tube connecting the first portion of the endoscope and the second portion of the endoscope may extend.

Abstract: Devices and methods for introducing a gas into a liquid are provided. Embodiments of the subject devices include spargers that have an inner member having a gas inlet opening and a gas outlet opening and an outer member that has at least one sparge hole. Embodiments of the subject methods include operatively positioning a sparger having an inner member and an outer member having at least one sparge hole inside a liquid held within a vessel and directing gas into the second member from the first member to cause the gas to exit the at least one sparger hole of the second member. Novel systems and kits are also provided.