Abstract: Various embodiments of the present disclosure provide therapeutic compositions and associated methods for improved hematopoietic stem cell transplantations, including methods to enhance protection from graft versus host disease while maintaining effective immune responses such as graft versus tumor immune responses.

Abstract: Embodiments of the disclosure provide compositions, kits, and methods for allogeneic hematopoietic cell transplantation (alloHCT) to patients. In an embodiment, a therapeutic composition for alloHCT includes at least first and second populations of isolated CD45+ cells (ICC). At least a portion of the CD45+ cells in the first population may have an antibody bound to a marker on the cell surface which is used to separate CD34+ cells from a mixture of nucleated cells (MNC) from donor or invitro produced blood. The MNC may comprise various cell types in various amounts, for example, about 70% CD34+ cells, less than about 5% CD3+ cells and less about 20% granulocytes. The ICC's in the second population include regulatory T cells which are typically at least about 50% of the population. Embodiments of the disclosure are particularly useful for treatment of hematologic cancers (e.g., leukemia, lymphoma), sickle cell anemia, GVHD, autoimmune and other diseases.

Abstract: Various embodiments of the invention provide therapeutic compositions and associated methods for improved hematopoietic stem cell transplantations, including methods to enhance protection from graft versus host disease while maintaining effective immune responses such as graft versus tumor immune responses. Embodiments of the invention are particularly useful for treatment of hematologic cancers (e.g., leukemia, lymphoma, GVHD and other diseases).

Abstract: The present invention is a brine-maker of a two-tank arrangement specifically designed for efficient removal of salt sludge. The first tank is a salt-hopper into which water flows from the bottom and up through salt to form brine and leaves mineral impurities called sludge on the bottom of the hopper. The brine then overflows through strainers into a brine containment tank. The outside of the salt hopper has bracketing means attached. When the sludge needs to be removed, a lifting device is attached to the bracketing means and the hopper is disconnected from the water supply by a quick-fit coupler and removed to a dumping site where it is turned on its side and the sludge is poured out. The salt hopper is then returned to its position and coupled to the water supply. The salinity level of the brine in the containment tank can be adjusted either by adding fresh water or by running the brine back through the salt in the salt hopper. The brine is stored in the brine containment tank until discharged for use.