Patents by Inventor Scott Kuersten
Scott Kuersten has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240150753Abstract: Described herein are compositions and methods for preparing double-stranded complementary DNA (cDNA) from RNA. In some embodiments, these methods allow isothermal preparation of cDNA. In some embodiments, these methods allow mesophilic or thermostable preparation of cDNA. Also described herein are compositions and methods for preparing cDNA and a library of double-stranded cDNA fragments in a single reaction vessel.Type: ApplicationFiled: September 27, 2023Publication date: May 9, 2024Applicant: Illumina, Inc.Inventors: Allison Yunghans, Angelica Marie Barr Schalembier, Kayla Busby, Stephen M. Gross, Robert Scott Kuersten, Frederick W. Hyde
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Patent number: 11976271Abstract: The present disclosure is related to methods and materials for depleting unwanted RNA species from a nucleic acid sample. In particular, the present disclosure describes how to remove unwanted rRNA, tRNA, mRNA or other RNA species that could interfere with the analysis, manipulation and study of target RNA molecules in a sample.Type: GrantFiled: July 14, 2022Date of Patent: May 7, 2024Assignee: Illumina, Inc.Inventors: Scott Kuersten, Frederick Hyde, Asako Tetsubayashi
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Publication number: 20240026348Abstract: Materials and methods for preparing nucleic acid libraries for next-generation sequencing are described herein. A variety of approaches are described relating to the use of unique molecular identifiers with transposon-based technology in the preparation of sequencing libraries. Also described herein are sequencing materials and methods for identifying and correcting amplification and sequencing errors.Type: ApplicationFiled: September 28, 2023Publication date: January 25, 2024Applicants: Illumina, Inc., Illumina Cambridge LimitedInventors: Susan C. Verity, Robert Scott Kuersten, Niall Anthony Gormley, Andrew B. Kennedy, Sarah E. Shultzaberger, Andrew Slatter, Emma Bell, Sebastien Georg Gabriel Ricoult, Grace DeSantis, Fiona Kaper, Han-Yu Chuang, Oliver Jon Miller, Jason Richard Betley, Stephen M. Gross, Mats Ekstrand
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Publication number: 20240003055Abstract: Presented herein are methods and compositions for analyzing rare nucleic acid species. Some methods presented herein use DNA reassociation kinetics following thermal denaturation to define populations of nucleic acid sequences, e.g., highly abundant (e.g., cDNA from rRNA), moderately abundant, and less abundant or rare sequences (e.g., cDNA from mRNA).Type: ApplicationFiled: September 6, 2023Publication date: January 4, 2024Applicant: ILLUMINA, INC.Inventors: Scott Kuersten, Agnes Radek, Ramesh Vaidyanathan, Haiying Li Grunenwald
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Patent number: 11795578Abstract: Presented herein are methods and compositions for analyzing rare nucleic acid species. Some methods presented herein use DNA reassociation kinetics following thermal denaturation to define populations of nucleic acid sequences, e.g., highly abundant (e.g., cDNA from rRNA), moderately abundant, and less abundant or rare sequences (e.g., cDNA from mRNA).Type: GrantFiled: April 25, 2022Date of Patent: October 24, 2023Assignee: Illumina, Inc.Inventors: Scott Kuersten, Agnes Radek, Ramesh Vaidyanathan, Haiying Li Grunenwald
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Publication number: 20230183682Abstract: This application describes methods of preparing an immobilized library of tagged RNA fragments. Also described herein are a number of methods of preparing DNA and RNA sequencing libraries from a single sample. These methods can include library preparation from single cells.Type: ApplicationFiled: February 2, 2023Publication date: June 15, 2023Applicants: Illumina, Inc., Illumina Cambridge LimitedInventors: Niall Anthony Gormley, Andrew B. Kennedy, Robert Scott Kuersten, Gary Schroth, Carlo Randise-Hinchliff, Sarah E. Shultzaberger, Fiona Kaper, Yir-Shyuan Wu, Tarun Khurana, Foad Mashayekhi
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Solid Supports and Methods for Depleting and/or Enriching Library Fragments Prepared from Biosamples
Publication number: 20230094911Abstract: Described herein are solid supports and methods for depleting library fragments prepared from unwanted RNA sequences and/or enriching library fragments prepared from desired RNA sequences. These methods may incorporate microfluidics and flowcells for greater ease of use. Libraries enriched or depleted with the present methods may be used for sequencing. Also described are probes and methods for enzymatic depletion of ribosomal RNA from human microbiome samples.Type: ApplicationFiled: September 30, 2022Publication date: March 30, 2023Applicant: ILLUMINA, INC.Inventors: Robert Scott Kuersten, Jeffrey Koble -
Publication number: 20220403370Abstract: The present disclosure is related to methods and materials for depleting unwanted RNA species from a nucleic acid sample. In particular, the present disclosure describes how to remove unwanted rRNA, tRNA, mRNA or other RNA species that could interfere with the analysis, manipulation and study of target RNA molecules in a sample.Type: ApplicationFiled: July 14, 2022Publication date: December 22, 2022Applicant: ILLUMINA, INC.Inventors: Scott Kuersten, Frederick Hyde, Asako Tetsubayashi
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Publication number: 20220275428Abstract: Presented herein are methods and compositions for analyzing rare nucleic acid species. Some methods presented herein use DNA reassociation kinetics following thermal denaturation to define populations of nucleic acid sequences, e.g., highly abundant (e.g., cDNA from rRNA), moderately abundant, and less abundant or rare sequences (e.g., cDNA from mRNA).Type: ApplicationFiled: April 25, 2022Publication date: September 1, 2022Applicant: Epicentre Technologies CorporationInventors: Scott Kuersten, Agnes Radek, Ramesh Vaidyanathan, Haiying Li Grunenwald
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Patent number: 11421216Abstract: The present disclosure is related to methods and materials for depleting unwanted RNA species from a nucleic acid sample. In particular, the present disclosure describes how to remove unwanted rRNA, tRNA, mRNA or other RNA species that could interfere with the analysis, manipulation and study of target RNA molecules in a sample.Type: GrantFiled: December 19, 2019Date of Patent: August 23, 2022Assignee: ILLUMINA, INC.Inventors: Scott Kuersten, Frederick W. Hyde, Asako Tetsubayashi
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Patent number: 11339422Abstract: Presented herein are methods and compositions for analyzing rare nucleic acid species. Some methods presented herein use DNA reassociation kinetics following thermal denaturation to define populations of nucleic acid sequences, e.g., highly abundant (e.g., cDNA from rRNA), moderately abundant, and less abundant or rare sequences (e.g., cDNA from mRNA).Type: GrantFiled: March 26, 2020Date of Patent: May 24, 2022Assignee: Illumina, Inc.Inventors: Scott Kuersten, Agnes Radek, Ramesh Vaidyanathan, Haiying Li Grunenwald
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Patent number: 10829808Abstract: Compositions, methods, and kits for detecting one or more species of RNA molecules are disclosed. In one embodiment, a first adaptor and a second adaptor are ligated to the RNA molecule using a polypeptide comprising double-strand specific RNA ligase activity, without an intervening purification step. The ligated product is reverse transcribed, then at least some of the ribonucleosides in the reverse transcription product are removed. Primers are added and amplified products are generated. In certain embodiments, the sequence of at least part of at least one species of amplified product is determined and at least part of the corresponding RNA molecule is determined. In some embodiments, at least some of the amplified product species are detected, directly or indirectly, allowing the presence and/or quantity of the RNA molecule of interest to be determined.Type: GrantFiled: March 25, 2019Date of Patent: November 10, 2020Assignee: Applied Biosystems, LLCInventor: R. Scott Kuersten
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Publication number: 20200255887Abstract: Presented herein are methods and compositions for analyzing rare nucleic acid species. Some methods presented herein use DNA reassociation kinetics following thermal denaturation to define populations of nucleic acid sequences, e.g., highly abundant (e.g., cDNA from rRNA), moderately abundant, and less abundant or rare sequences (e.g., cDNA from mRNA).Type: ApplicationFiled: March 26, 2020Publication date: August 13, 2020Applicant: EPICENTRE TECHNOLOGIES CORPORATIONInventors: Scott Kuersten, Agnes Radek, Ramesh Vaidyanathan, Haiying Grunenwald
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Publication number: 20200199572Abstract: The present disclosure is related to methods and materials for depleting unwanted RNA species from a nucleic acid sample. In particular, the present disclosure describes how to remove unwanted rRNA, tRNA, mRNA or other RNA species that could interfere with the analysis, manipulation and study of target RNA molecules in a sample.Type: ApplicationFiled: December 19, 2019Publication date: June 25, 2020Applicant: EPICENTRE TECHNOLOGIES CORPORATIONInventors: Scott Kuersten, Frederick W. Hyde, Asako Tetsubayashi
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Patent number: 10640809Abstract: Presented herein are methods and compositions for analyzing rare nucleic acid species. Some methods presented herein use DNA reassociation kinetics following thermal denaturation to define populations of nucleic acid sequences, e.g., highly abundant (e.g., cDNA from rRNA), moderately abundant, and less abundant or rare sequences (e.g., cDNA from mRNA).Type: GrantFiled: May 27, 2016Date of Patent: May 5, 2020Assignee: EPICENTRE TECHNOLOGIES CORPORATIONInventors: Scott Kuersten, Agnes Radek, Ramesh Vaidyanathan, Haiying Li Grunenwald
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Patent number: 10544403Abstract: Presented herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction conditions which exhibit improved insertion sequence bias, as well as methods and kits using the same.Type: GrantFiled: April 29, 2019Date of Patent: January 28, 2020Assignee: ILLUMINA, INC.Inventors: Christian Gloeckner, Amirali Kia, Erin Bomati, Molly He, Haiying Li Grunenwald, Scott Kuersten, Trina Faye Osothprarop, Darin Haskins, Joshua Burgess, Anupama Khanna, Daniel Schlingman, Ramesh Vaidyanathan
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Publication number: 20190359955Abstract: Presented herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction conditions which exhibit improved insertion sequence bias, as well as methods and kits using the same.Type: ApplicationFiled: April 29, 2019Publication date: November 28, 2019Inventors: Christian GLOECKNER, Amirali KIA, Erin BOMATI, Molly HE, Haiying Li GRUNENWALD, Scott KUERSTEN, Trina Faye OSOTHPRAROP, Darin HASKINS, Joshua BURGESS, Anupama KHANNA, Daniel SCHLINGMAN, Ramesh VAIDYANATHAN
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Patent number: 10385323Abstract: Presented herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction conditions which exhibit improved insertion sequence bias, as well as methods and kits using the same.Type: GrantFiled: June 28, 2018Date of Patent: August 20, 2019Assignee: ILLUMINA, INC.Inventors: Christian Gloeckner, Amirali Kia, Erin Bomati, Molly He, Haiying Li Grunenwald, Scott Kuersten, Trina Faye Osothprarop, Darin Haskins, Joshua Burgess, Anupama Khanna, Daniel Schlingman, Ramesh Vaidyanathan
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Publication number: 20190211387Abstract: Compositions, methods, and kits for detecting one or more species of RNA molecules are disclosed. In one embodiment, a first adaptor and a second adaptor are ligated to the RNA molecule using a polypeptide comprising double-strand specific RNA ligase activity, without an intervening purification step. The ligated product is reverse transcribed, then at least some of the ribonucleosides in the reverse transcription product are removed. Primers are added and amplified products are generated. In certain embodiments, the sequence of at least part of at least one species of amplified product is determined and at least part of the corresponding RNA molecule is determined. In some embodiments, at least some of the amplified product species are detected, directly or indirectly, allowing the presence and/or quantity of the RNA molecule of interest to be determined.Type: ApplicationFiled: March 25, 2019Publication date: July 11, 2019Inventor: R. Scott KUERSTEN
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Patent number: 10240191Abstract: Compositions, methods, and kits for detecting one or more species of RNA molecules are disclosed. In one embodiment, a first adaptor and a second adaptor are ligated to the RNA molecule using a polypeptide comprising double-strand specific RNA ligase activity, without an intervening purification step. The ligated product is reverse transcribed, then at least some of the ribonucleosides in the reverse transcription product are removed. Primers are added and amplified products are generated. In certain embodiments, the sequence of at least part of at least one species of amplified product is determined and at least part of the corresponding RNA molecule is determined. In some embodiments, at least some of the amplified product species are detected, directly or indirectly, allowing the presence and/or quantity of the RNA molecule of interest to be determined.Type: GrantFiled: November 3, 2017Date of Patent: March 26, 2019Assignee: Applied Biosystems, LLCInventor: R. Scott Kuersten