Abstract: Provided herein are methods of delivering therapeutic agents, such as synthetic circular DNA vectors, to target airway cells (e.g., airway epithelial cells). Such methods can be useful in treating respiratory diseases or disorders, such as cystic fibrosis.

Abstract: Provided are epithelial cell spheroids including spheroids that have apical membranes and cilia that face towards the interior core of the spheroid and spheroids that have apical membranes and cilia that face away from the interior core of the spheroid. Also provided methods of making and using such spheroids.

Abstract: Provided are epithelial cell spheroids including spheroids that have apical membranes and cilia that face towards the interior core of the spheroid and spheroids that have apical membranes and cilia that face away from the interior core of the spheroid. Also provided methods of making and using such spheroids.

Abstract: A system and method for efficiently adding a new network resource to a digital video network, without the need to purchase costly ancillary network hardware or extensive manual configuration, is disclosed. The inventive system and method provide for a loop-through for adding new resources to a digital video network. The present invention provides for the resources to be daisy chained from the network switch, where each resource is configured simply as either a loop-through (“LT”) resource or the “last” resource in the daisy chain. Then, only upstream traffic with Media Access Control (“MAC”) addresses of upstream LT resources are transmitted out of the loop-through by the last network resource.

Abstract: A system and method for efficiently adding a new network resource to a digital video network, without the need to purchase costly ancillary network hardware or extensive manual configuration, is disclosed. The inventive system and method provide for a loop-through for adding new resources to a digital video network. The present invention provides for the resources to be daisy chained from the network switch, where each resource is configured simply as either a loop-through (“LT”) resource or the “last” resource in the daisy chain. Then, only upstream traffic with Media Access Control (“MAC”) addresses of upstream LT resources are transmitted out of the loop-through by the last network resource.

Abstract: Wireless communication protocol for enabling unidirectional communications in a simplex environment, or bidirectional communications in a duplex environment, between a controller and one or more controlled devices. The controller transmits video data and control data that may include voice synthesis coefficients and motor movement coefficients for processing by the controlled devices. The video data drives a display device to depict a scene, and the control data drives the controlled devices so that the controlled devices behave as characters in the scene depicted on the display device. Each controlled device is operable for recognizing messages directed to the device and for parsing these messages into control signals for a number of end effectors, such as speech synthesizers, servo motors, lights, heat sources, pumps, etc.

Abstract: A communications system that provides bandwidth efficient, bi-directional communications over a single communications channel, allowing a master device to control the operation of remote devices. A computer system continuously provides an audio/video presentation on a speaker and display device. During a first time period, the computer system also provides control and speech data, relevant to the presentation, to a link master controller. The link master controller encodes the control and speech data to reduce bandwidth requirements, and then transmits the encoded data to a remote device. The remote device receives and decodes the encoded data, places the data into a data buffer, and uses the data to control at least one motion servo motor and/or provide data to a speech synthesizer.

Abstract: A minimal rebuild system and process for minimizing rebuilding of a user's programming project analyzes and records dependencies of object code files compiled in a previous build of the project on classes declared in header files. When rebuilding the project, the system detects and records changes made to the classes and header files since the project was last built. The system then determines whether to recompile the object code files from their respective source code files by comparing the recorded dependencies and changes. If an object code file's dependencies do not intersect the changes, recompiling of the object code file can be omitted. The minimal rebuild system utilizes an approximate representation of the dependencies to yield an efficient system while ensuring that the project is correctly rebuilt.