Abstract: Described herein are methods and compositions for improved polymerase chain reaction (PCR). In one aspect the methods and compositions include improved aptamers to reversibly inhibit polymerase and exonuclease activity of the polymerase enzyme.

Abstract: The invention can be used to provide a more efficient and less error-prone method of detecting variants in DNA, such as SNPs and indels. The invention also provides a method for performing inexpensive multiplex assays.

Abstract: The present invention pertains to a kit for producing an extended primer, comprising at least one container providing a hybrid RNase H2 protein, including a hybrid RNase H2 protein comprising fragments of amino acid sequences from Pyrococcus abyssi (P. a.), Thermococcus kodakarensis (T. kod), and Pyrococcus furiosus organisms; a hybrid RNase H2 protein comprises amino acid residues 26-40 and residues 100-120 of T. kod RNase H2; a hybrid RNase H2 protein is selected from SEQ ID NO: 2 and 3; and a hybrid RNase H2 protein is selected from SEQ ID NO: 14-20.

Abstract: The present invention pertains to hybrid RNase H2 proteins that include fragments of amino acid sequences from Pyrococcus abyssi (P.a.), Thermococcus kodakarensis (T.kod), and Pyrococcus furiosus organisms, as well as methods of using the same to improve mismatch discrimination and activity in a high-fidelity DNA polymerase buffer.

Abstract: An endotracheal tube placement system for securing an endotracheal tube relative to a patient's vocal chords during surgery is disclosed. The system can include a clamp that receives the endotracheal tube and a clamp lock that selectively retains the clamp in a clamped position holding the endotracheal tube in place relative to the clamp. The clamp and clamp lock can be fixed to the mouth area of the patient's face using tape strips. The system may also include positioners sized to be placed between the upper and lower teeth of the patient when the clamp is fixed to a patient's face.

Abstract: Described herein are methods and compositions for improved polymerase chain reaction (PCR). In one aspect the methods and compositions include improved aptamers to reversibly inhibit polymerase and exonuclease activity of the polymerase enzyme.

Abstract: The present invention pertains to hybrid RNase H2 proteins that include fragments of amino acid sequences from Pyrococcus abyssi (P.a.), Thermococcus kodakarensis (T.kod), and Pyrococcus furiosus organisms, as well as methods of using the same to improve mismatch discrimination and activity in a high-fidelity DNA polymerase buffer.

Abstract: The invention can be used to provide a more efficient and less error-prone method of detecting variants in DNA, such as SNPs and indels. The invention also provides a method for performing inexpensive multiplex assays.

Abstract: A network apparatus and a content distribution apparatus are described. The network apparatus is configured to transcode and transmit pre-broadcast content into transcoded content according to a format associated with a content distribution apparatus. The transcoded content includes a distribution indicator to restrict distribution of the transcoded content to one or more remote apparatus. Network apparatus is configured to transmit a “delta file” to content distribution apparatus with transcoded content and instructions to signal modifying the pre-broadcast content. Content distribution apparatus is configured to receive transcoded pre-broadcast content and “delta file” with instructions from network apparatus. Content distribution apparatus removes the distribution indicator from the transcoded pre-broadcast content once the signaling instructions indicates that the broadcast content corresponds with pre-broadcast content.

Abstract: The invention provides methods for the synthesis of long oligonucleotides, genes and gene fragments. The methods include the manufacture of genes or gene fragments that can be then inserted into a variety of vectors.

Abstract: A network apparatus and a content distribution apparatus are described. The network apparatus is configured to transcode and transmit pre-broadcast content into transcoded content according to a format associated with a content distribution apparatus. The transcoded content includes a distribution indicator to restrict distribution of the transcoded content to one or more remote apparatus. Network apparatus is configured to transmit a “delta file” to content distribution apparatus with transcoded content and instructions to signal modifying the pre-broadcast content. Content distribution apparatus is configured to receive transcoded pre-broadcast content and “delta file” with instructions from network apparatus. Content distribution apparatus removes the distribution indicator from the transcoded pre-broadcast content once the signaling instructions indicates that the broadcast content corresponds with pre-broadcast content.

Abstract: A network apparatus and a content distribution apparatus are described. The network apparatus is configured to transcode and transmit pre-broadcast content into transcoded content according to a format associated with a content distribution apparatus. The transcoded content includes a distribution indicator to restrict distribution of the transcoded content to one or more remote apparatus. Network apparatus is configured to transmit a “delta file” to content distribution apparatus with transcoded content and instructions to signal modifying the pre-broadcast content. Content distribution apparatus is configured to receive transcoded pre-broadcast content and “delta file” with instructions from network apparatus. Content distribution apparatus removes the distribution indicator from the transcoded pre-broadcast content once the signaling instructions indicates that the broadcast content corresponds with pre-broadcast content.

Abstract: The present invention pertains to novel oligonucleotide compounds for use in various biological assays, such as nucleic acid amplification, ligation and sequencing reactions. The novel oligonucleotides comprise a ribonucleic acid domain and a blocking group at or near the 3? end of the oligonucleotide. These compounds offer an added level of specificity previously unseen. Methods for performing nucleic acid amplification, ligation and sequencing are also provided. Additionally, kits containing the oligonucleotides are also disclosed herein.

Abstract: A conveyor system includes a product distribution conveyor which spreads products across its width and aligns them in uniform orientations while doing so; a lateral transfer conveyor which receives a row of the aligned products from the product distribution conveyor, and rejects unaligned and/or excess products while doing so; and a product loading conveyor which receives each row of products and loads them into packaging in a neatly aligned array.

Abstract: This invention relates to primers and probes for detecting Ebola virus and one or more subtypes of Ebola virus as well as kits including the probes and primers and methods of using the probes and primers.

Abstract: This invention pertains to improved methods for the synthesis of long, double stranded nucleic acid sequences containing difficult to clone or variable regions.

Abstract: The present invention pertains to novel oligonucleotide compounds for use in various biological assays, such as nucleic acid amplification, ligation and sequencing reactions. The novel oligonucleotides comprise a ribonucleic acid domain and a blocking group at or near the 3? end of the oligonucleotide. These compounds offer an added level of specificity previously unseen. Methods for performing nucleic acid amplification, ligation and sequencing are also provided. Additionally, kits containing the oligonucleotides are also disclosed herein.

Abstract: The invention provides methods for the synthesis of long oligonucleotides, genes and gene fragments. The methods include the manufacture of genes or gene fragments that can be then inserted into a variety of vectors.

Abstract: A network apparatus and a content distribution apparatus are described. The network apparatus is configured to transcode and transmit pre-broadcast content into transcoded content according to a format associated with a content distribution apparatus. The transcoded content includes a distribution indicator to restrict distribution of the transcoded content to one or more remote apparatus. Network apparatus is configured to transmit a “delta file” to content distribution apparatus with transcoded content and instructions to signal modifying the pre-broadcast content. Content distribution apparatus is configured to receive transcoded pre-broadcast content and “delta file” with instructions from network apparatus. Content distribution apparatus removes the distribution indicator from the transcoded pre-broadcast content once the signaling instructions indicates that the broadcast content corresponds with pre-broadcast content.

Abstract: The invention provides methods for the synthesis of long oligonucleotides, genes and gene fragments. The methods include the manufacture of genes or gene fragments that can be then inserted into a variety of vectors.