Abstract: Aspects of the invention relate to methods, compositions and algorithms for designing and producing a target nucleic acid. The method can include: (1) providing a plurality of blunt-end double-stranded nucleic acid fragments having a restriction enzyme recognition sequence at both ends thereof; (2) producing via enzymatic digestion a plurality of cohesive-end double-stranded nucleic acid fragments each having two different and non-complementary overhangs; (3) ligating the plurality of cohesive-end double-stranded nucleic acid fragments with a ligase; and (4) forming a linear arrangement of the plurality of cohesive-end double-stranded nucleic acid fragments, wherein the unique arrangement comprises the target nucleic acid.

Abstract: Aspects of the invention relate to methods, compositions and algorithms for designing and producing a target nucleic acid. The method can include: (1) providing a plurality of blunt-end double-stranded nucleic acid fragments having a restriction enzyme recognition sequence at both ends thereof; (2) producing via enzymatic digestion a plurality of cohesive-end double-stranded nucleic acid fragments each having two different and non-complementary overhangs; (3) ligating the plurality of cohesive-end double-stranded nucleic acid fragments with a ligase; and (4) forming a linear arrangement of the plurality of cohesive-end double-stranded nucleic acid fragments, wherein the unique arrangement comprises the target nucleic acid.

Abstract: Aspects of the invention relate to methods, compositions for synthesizing oligonucleotides having a predefined sequence.

Abstract: Aspects of the invention relate to methods, compositions for synthesizing oligonucleotides having a predefined sequence.

Abstract: Aspects of the invention relate to methods, compositions and algorithms for designing and producing a target nucleic acid. The method can include: (1) providing a plurality of blunt-end double-stranded nucleic acid fragments having a restriction enzyme recognition sequence at both ends thereof; (2) producing via enzymatic digestion a plurality of cohesive-end double-stranded nucleic acid fragments each having two different and non-complementary overhangs; (3) ligating the plurality of cohesive-end double-stranded nucleic acid fragments with a ligase; and (4) forming a linear arrangement of the plurality of cohesive-end double-stranded nucleic acid fragments, wherein the unique arrangement comprises the target nucleic acid.

Abstract: Aspects of the invention relate to methods, compositions for designing and producing a target nucleic acid. In particular, aspects of the invention relate to the multiplex synthesis of target polynucleotides.

Abstract: Aspects of the invention relate to methods, compositions for designing and producing a target nucleic acid. In particular, aspects of the invention relate to the multiplex synthesis of target polynucleotides.

Abstract: Aspects of the invention relate to methods, compositions for synthesizing oligonucleotides having a predefined sequence.

Abstract: Aspects of the invention relate to methods, compositions for designing and producing a target nucleic acid. In particular, aspects of the invention relate to the multiplex synthesis of target polynucleotides.

Abstract: Aspects of the invention relate to methods, compositions for synthesizing oligonucleotides having a predefined sequence.

Abstract: Aspects of the invention relate to methods, compositions for synthesizing oligonucleotides having a predefined sequence.

Abstract: Aspects of the invention relate to methods, compositions and algorithms for designing and producing a target nucleic acid. The method can include: (1) providing a plurality of blunt-end double-stranded nucleic acid fragments having a restriction enzyme recognition sequence at both ends thereof; (2) producing via enzymatic digestion a plurality of cohesive-end double-stranded nucleic acid fragments each having two different and non-complementary overhangs; (3) ligating the plurality of cohesive-end double-stranded nucleic acid fragments with a ligase; and (4) forming a linear arrangement of the plurality of cohesive-end double-stranded nucleic acid fragments, wherein the unique arrangement comprises the target nucleic acid.

Abstract: Aspects of the invention relate to methods, compositions and algorithms for designing and producing a target nucleic acid. The method can include: (1) providing a plurality of blunt-end double-stranded nucleic acid fragments having a restriction enzyme recognition sequence at both ends thereof; (2) producing via enzymatic digestion a plurality of cohesive-end double-stranded nucleic acid fragments each having two different and non-complementary overhangs; (3) ligating the plurality of cohesive-end double-stranded nucleic acid fragments with a ligase; and (4) forming a linear arrangement of the plurality of cohesive-end double-stranded nucleic acid fragments, wherein the unique arrangement comprises the target nucleic acid.

Abstract: A method and system for automatically saving a user's search criteria and pre-filling the search criteria into the search form on the user's next visit. The invention saves the user from having to remember exactly what search criteria was previously entered, or from having to reenter that search criteria, or from having to explicitly store and recall that search criteria.

Abstract: A method and system for modifying stored search criteria so that it will locate only those data objects that have been added and/or updated since the date on which the user previously invoked the stored search criteria. This invention reduces the time and frustration associated with repeated searches, by automatically showing only new or modified data objects with no additional effort required by the user.

Abstract: An enhanced method and system for storing and managing search criteria, streamlining the process by eliminating one step and integrating other innovations to increase user convenience. This invention reduces the time and frustration associated with repeated searches, by reducing the time required to store, review, edit, delete and invoke search criteria.

Abstract: A method and system for automatically saving a user's search criteria and pre-filling the search criteria into the search form on the user's next visit. The invention saves the user from having to remember exactly what search criteria was previously entered, or from having to reenter that search criteria, or from having to explicitly store and recall that search criteria.

Abstract: An enhanced method and system for viewing and selecting from multiple options for a single data element, where all options are visible and organized into subgroups, whereby the user can select or unselect any option with only a single click, no modifier key required and without affecting any other option and can quickly locate the appropriate options and determine at a glance which options are selected.

Abstract: A method and system whereby a user is presented with a visually clear and meaningful view of data objects, organized according to the user's preferred criteria; thereby helping the user understand the scope and content of the information. A computer with appropriate software formats the information based on user-supplied grouping criteria.

Abstract: A method and system whereby users can control the primary sort criteria while retaining the benefit of also sorting by non-redundant portions of default sort criteria and/or by additional criteria that is implied by the user's explicit choice; in either case reducing the time and effort spent finding and understanding information. A computer with appropriate software adapts the default and/or implied sort criteria to fit the user-supplied sort criteria.