Abstract: A wedge fastener configured for use in extreme ambient conditions includes a pin or bolt having a head and a shank with an elongate slot through the shank. The wedge fastener also includes a wedge sized so that at least a portion of the wedge may extend through the elongate slot to grip a flange between the head and the wedge. The wedge fastener also includes a locking mechanism for locking the wedge in a gripping position against the flange. In one example, the locking mechanism includes locking keys with angled ridges configured to slide in angled slots on the wedge until the locking keys engage the shank. In another example, the locking mechanism includes a jack plate attached to the wedge and adjustment screws for engaging the shank to pull the wedge through the elongate slot.

Abstract: A wedge fastener configured for use in extreme ambient conditions includes a pin or bolt having a head and a shank with an elongate slot through the shank. The wedge fastener also includes a wedge sized so that at least a portion of the wedge may extend through the elongate slot to grip a flange between the head and the wedge. The wedge fastener also includes a locking mechanism for locking the wedge in a gripping position against the flange. In one example, the locking mechanism includes locking keys with angled ridges configured to slide in angled slots on the wedge until the locking keys engage the shank. In another example, the locking mechanism includes a jack plate attached to the wedge and adjustment screws for engaging the shank to pull the wedge through the elongate slot.

Abstract: The present invention recognizes that marine organisms comprise nucleic acid molecules that encode polypeptides that catalyze the synthesis of bioactive compounds, such as polyketides including bryopyran rings, such as bryostatins. One aspect of the present invention is a composition including at least one nucleic acid molecule that encodes at least one polypeptide that catalyzes at least one step in the synthesis of at least one polyketide such as a bryopyran ring, wherein said at least one nucleic acid molecule is derived from at least one marine organism. A second aspect of the present invention is a composition including a library of nucleic acid molecules of the present invention. These nucleic acid molecules can be used in a combinatorial biosynthesis of polyketides, bryopyran rings and bryostatins.