Abstract: Systems and methods for flattening the image across the entire field by correcting the image for both the fluorescence and scatter spatial variations. Images of a stable fluorescence target and a scattering target are separately acquired in an imaging system. From these target images, a pixel remapping function, e.g., including two correcting pixel slopes, is calculated for subsequent image pixel remapping. An image of a sample under investigation is then acquired by the imaging system and the sample image is remapped based on the pixel remapping function for the imaging system to form a corrected (field flattened) image. Which correction pixel slope to be used is determined based on whether a sample image pixel value is higher or lower than a threshold value.

Abstract: A measurement system for use with fluorescent chemosensors has multiple stimulus light sources each coupled to at least one sensor. Multiple sensors each receiving light from a different light source connect to each of one or more photodetectors. A processing device drives the light sources in a time-division multiplexed manner, and reads the photodetector at an appropriate time for each sensor. The processing device calibrates the sensor readings and provides them in a way that is identified to the associated sensor.

Abstract: A fluorescence imaging system having an enclosure having an optical excitation and detection system and features designed to suppress or reduce background fluorescence. In certain aspects, all or a portion of the interior walls has a material finish and texture that provides a surface that absorbs at least a portion of any impinging excitation light and which has low auto-fluorescing properties. In certain aspects, a baffle structure is provided on the interior of the structure and is configured to mask portions of the interior and reduce the opening through which light impinges on the detector.

Abstract: A fluorescence imaging system having an enclosure having an optical excitation and detection system and features designed to suppress or reduce background fluorescence. In certain aspects, all or a portion of the interior walls has a material finish and texture that provides a surface that absorbs at least a portion of any impinging excitation light and which has low auto-fluorescing properties. In certain aspects, a baffle structure is provided on the interior of the structure and is configured to mask portions of the interior and reduce the opening through which light impinges on the detector.

Abstract: A measurement system for use with fluorescent chemosensors has multiple stimulus light sources each coupled to at least one sensor. Multiple sensors each receiving light from a different light source connect to each of one or more photodetectors. A processing device drives the light sources in a time-division multiplexed manner, and reads the photodetector at an appropriate time for each sensor. The processing device calibrates the sensor readings and provides them in a way that is identified to the associated sensor.

Abstract: A lens system for collecting and focusing light emanating from an object has a cuvette housing the object and having a wall of thickness not greater than 1.5 millimeters; a plano-convex lens having a planar surface affixed to the wall; a sequence of at least three meniscus lenses, each meniscus lens having a concave surface toward the object and a convex surface, each successive meniscus lens receiving the light from the immediately preceding meniscus lens and having radii of curvature of its concave and convex surfaces greater than corresponding radii of the preceding meniscus lens; and at least one compound lens that may be a doublet lens or a triplet lens, the compound lens receiving the light from a last meniscus lens, wherein an image of a geometrical point on the object has a root-mean square spot size equal to or less than 63 ?m.