Abstract: Compositions and methods comprising polynucleotides and polypeptides having ALS activity and tolerance to at least one ALS inhibitor are provided. In specific embodiments, the sequence has an increased preference for 2-ketobutyrate, when compared to an appropriate control, such as for example, HRA, and/or a preference for 2-ketobutyrate similar to a native ALS. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the ALS inhibitor tolerant sequences. Various methods of employing the ALS inhibitor tolerant sequences are provided. Such methods include methods for producing an ALS inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.

Abstract: Compositions and methods comprising polynucleotides and polypeptides having ALS activity and tolerance to at least one ALS inhibitor are provided. In specific embodiments, the sequence has an increased preference for 2-ketobutyrate, when compared to an appropriate control, such as for example, HRA, and/or a preference for 2-ketobutyrate similar to a native ALS. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the ALS inhibitor tolerant sequences. Various methods of employing the ALS inhibitor tolerant sequences are provided. Such methods include methods for producing an ALS inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.

Abstract: The present invention provides recombinant expression cassettes comprising a fungal 3? termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop codon, and fungal termination sequence. The fungal 3? termination sequence is heterologous to the coding sequence. The fungal 3? termination sequence comprises structural features including a cleavage site, a positioning element. and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

Abstract: The present invention provides recombinant expression cassettes comprising a fungal 3? termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop codon, and the fungal termination sequence. The fungal 3? termination sequence is heterologous to the coding sequence. The fungal 3? termination sequence comprises structural features including a cleavage site, a positioning element and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

Abstract: The present invention provides recombinant expression cassettes comprising a fungal 3? termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop codon, and the fungal termination sequence. The fungal 3? termination sequence is heterologous to the coding sequence. The fungal 3? termination sequence comprises structural features including a cleavage site, a positioning element, and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

Abstract: The present invention provides recombinant expression cassettes comprising a fungal 3? termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop condon, and the fungal termination sequence. The fungal 3? termination sequence is heterologous to the coding sequence. The fungal 3? termination sequence comprises structural features including a cleavage site, a positioning element, and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

Abstract: The present invention provides recombinant expression cassettes comprising a fungal 3? termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop codon, and the fungal termination sequence. The fungal 3? termination sequence is heterologous to the coding sequence. The fungal 3? termination sequence comprises structural features including a cleavage site, a positioning element and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

Abstract: The present invention provides recombinant expression cassettes comprising a fungal 3? termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop codon, and the fungal termination sequence. The fungal 3? termination sequence is heterologous to the coding sequence. The fungal 3? termination sequence comprises structural features including a cleavage site, a positioning element and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

Abstract: The present invention provides recombinant expression cassettes comprising a fungal 3? termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop codon, and the fungal termination sequence. The fungal 3? termination sequence is heterologous to the coding sequence. The fungal 3? termination sequence comprises structural features including a cleavage site, a positioning element, and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

Abstract: The present invention relates to heterologous genes comprising non-plant 3? termination sequences and plant expression cassettes incorporating the heterologous genes. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

Abstract: The present invention relates to heterologous genes comprising non-plant 3? termination sequences and plant expression cassettes incorporating the heterologous genes. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

Abstract: The present invention relates to heterologous genes comprising non-plant 3? termination sequences and plant expression cassettes incorporating the heterologous genes. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

Abstract: The present invention relates to heterologous genes comprising non-plant 3? termination sequences and plant expression cassettes incorporating the heterologous genes. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

Abstract: The present invention relates to heterologous genes comprising non-plant 3? termination sequences and plant expression cassettes incorporating the heterologous genes. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

Abstract: The present invention relates to heterologous genes comprising non-plant 3? termination sequences and plant expression cassettes incorporating the heterologous genes. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

Abstract: The present invention provides recombinant expression cassettes comprising a fungal 3? termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop codon, and the fungal 3? termination sequence. The fungal 3? termination sequence is heterologous to the coding sequence. The fungal 3? termination sequence comprises structural features including a cleavage site, a positioning element, and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

Abstract: The present invention is directed to a vector for identifying read-through of non-T-DNA in a T-DNA vector. In one embodiment, the vector provides a visually detectable change in the normal appearance of transformants wherein read-through has occurred. In another embodiment, the vector also provides for expression of a readily detectable fluorescent protein that allows for the early detection and elimination of transformants wherein read-through has occurred. In a further aspect, the present invention is directed to a method for detecting read-through of non-T-DNA in plants transformed with a T-DNA vector. In another aspect, the present invention is directed to a method for producing a transgenic plant containing a polynucleotide of interest but being substantially free of non-T-DNA.

Abstract: The present invention relates to heterologous genes comprising non-plant 3′ termination sequences and plant expression cassettes incorporating the heterologous genes. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.