Abstract: Acetate is a potent microbial inhibitor which can affect the performance of yeast in ethanolic fermentation. The present disclosure provides a recombinant microbial host cell having (i) a first genetic modification for increasing the activity of one or more proteins that function in a first metabolic pathway to convert acetate into an alcohol in the microbial host cell; (ii) a second genetic modification for increasing the activity of one or more proteins that function in a second metabolic pathway to import glycerol in the recombinant microbial host cell (iii) a third genetic modification for increasing the activity of one or more proteins that function in a third metabolic pathway to convert a C5 carbohydrate into ethanol in the microbial host cell. The recombinant microbial host cell comprises and natively expresses native proteins that function in a fourth native metabolic pathway to produce glycerol in the microbial host cell.

Abstract: There is provided an engineered host cells comprising (a) one or more mutations in one or more endogenous genes encoding a protein associated with iron metabolism; and (b) at least one gene encoding a polypeptide having xylose isomerase activity, and methods of their use thereof.

Abstract: Acetate is a potent microbial inhibitor which can affect the performance of yeast in ethanolic fermentation. The present disclosure provides a recombinant microbial host cell having (i) a first genetic modification for increasing the activity of one or more proteins that function in a first metabolic pathway to convert acetate into an alcohol in the microbial host cell; (ii) a second genetic modification for increasing the activity of one or more proteins that function in a second metabolic pathway to import glycerol in the recombinant microbial host cell (iii) a third genetic modification for increasing the activity of one or more proteins that function in a third metabolic pathway to convert a C5 carbohydrate into ethanol in the microbial host cell. The recombinant microbial host cell comprises and natively expresses native proteins that function in a fourth native metabolic pathway to produce glycerol in the microbial host cell.

Abstract: There is provided an engineered host cells comprising (a) one or more mutations in one or more endogenous genes encoding a protein associated with iron metabolism; and (b) at least one gene encoding a polypeptide having xylose isomerase activity, and methods of their use thereof.

Abstract: A support that comprises a plate having a first side and a second side opposed to the first side. The plate has a sample section including bottle-receiving members defined in the first side and configured for holding the bottles in a fixed position relative to the plate, and first conduit-receiving openings extending from the first side to the second side for receiving conduits extending from the bottles. The plate has a manifold section including second conduit-receiving openings extending from the first side to the second side for receiving the conduits. The plate has a channel section including channels defined in the second side and extending between the first conduit-receiving openings and the second conduit-receiving openings for receiving the conduits therein. The support may be used for example on a shaker. A method for retaining bottles and conduits connected to the bottles is also discussed.

Abstract: There is provided an engineered host cells comprising (a) one or more mutations in one or more endogenous genes encoding a protein associated with iron metabolism; and (b) at least one gene encoding a polypeptide having xylose isomerase activity, and methods of their use thereof.

Abstract: A support that comprises a plate having a first side and a second side opposed to the first side. The plate has a sample section including bottle-receiving members defined in the first side and configured for holding the bottles in a fixed position relative to the plate, and first conduit-receiving openings extending from the first side to the second side for receiving conduits extending from the bottles. The plate has a manifold section including second conduit-receiving openings extending from the first side to the second side for receiving the conduits. The plate has a channel section including channels defined in the second side and extending between the first conduit-receiving openings and the second conduit-receiving openings for receiving the conduits therein. The support may be used for example on a shaker. A method for retaining bottles and conduits connected to the bottles is also discussed.

Abstract: There is provided an engineered host cells comprising (a) one or more mutations in one or more endogenous genes encoding a protein associated with iron metabolism; and (b) at least one gene encoding a polypeptide having xylose isomerase activity, and methods of their use thereof.

Abstract: There is provided an engineered host cells comprising (a) one or more mutations in one or more endogenous genes encoding a protein associated with iron metabolism; and (b) at least one gene encoding a polypeptide having xylose isomerase activity, and methods of their use thereof.

Abstract: There is provided an engineered host cells comprising (a) one or more mutations in one or more endogenous genes encoding a protein associated with iron metabolism; and (b) at least one gene encoding a polypeptide having xylose isomerase activity, and methods of their use thereof.

Abstract: The invention is directed to the heterologous expression of urease in anaerobic thermophilic hosts, such as Thermoanaerobacterium, Thermoanaerobacter, and other related genera. For example, the anaerobic thermophilic host can be T. saccharolyticum. The host cells express the catalytic subunits of the urease enzyme together with the accessory proteins ureDEFG that facilitate protein folding and nickel activation. The invention further relates to the use of urea as a nitrogen source in the growth of microorganisms involved in consolidated bioprocessing systems.