Abstract: Methods of purifying a heterodimeric protein (e.g., a bispecific antibody) from impurities through a series of chromatographic cycles are disclosed. In various embodiments, the pH of the elution buffer is increased with increasing cycles within the series to maintain minimal contamination with a binding impurity, and without significant loss of recovery of the heterodimeric protein.

Abstract: A system and method of performing mass spectrometry analysis on a sample such as a cellular component subject to laser desorption ionization is disclosed. A cell sample is lysed. An antibody is attached to a columnar array. The lysed cell sample for analysis is placed on the columnar array. The antibody retains components of the cell sample on the array to selectively capture cellular components of the cell sample. A laser is used to desorb and ionize the components of the cell sample. Mass spectrometry is performed on the ionized cell components.

Abstract: Compounds of formula I wherein n, m, p, q, Y, R1 R2, R3a, R3b, R4, R5, and R6 are as defined herein and their pharmaceutical compositions and methods of use are disclosed.