Abstract: Provided herein are engineered Class 2, Type V nucleases and guide RNAs useful for the editing of target nucleic acids. Also provided are methods of making and using such variants to modify nucleic acids.

Abstract: Provided herein are reference guide nucleic acid scaffolds and variants of reference guide nucleic acid scaffolds capable of binding one or more engineered proteins comprising a RuvC cleavage domain. In some embodiments, the variants of the reference guide nucleic acid scaffolds comprise at least one modification compared to the reference guide nucleic acid scaffold sequences and exhibit one or more improved characteristics compared to the reference guide nucleic acid scaffolds.

Abstract: Provided herein are delivery particle systems (XDP) useful for the delivery of payloads of any type. In some embodiments, a XDP particle system with tropism for target cells of interest is used to deliver CRISPR/Cas polypeptides (e.g., CasX proteins) and guide nucleic acids (gNA), for the modification of nucleic acids in target cells. Also provided are methods of making and using such XDP to modify the nucleic acids in such cells.

Abstract: According to example embodiments, there is provided a handheld mobile computing device, for example a smartphone, includes a computing system, a motion detector, a global positioning system receiver, and a touch-screen display to display a user interface and detect user selections from the user interface. A usage goal-setting user interface includes a dashboard and a plurality of user-selectable menu options, the usage goal-setting user interface including display elements allowing a user to enter one or more parameters respecting the goals including physical activity usage goals, social activity usage goals, educational usage goals, and mindfulness usage goals.

Abstract: Provided herein are CasX:gNA systems comprising CasX polypeptides, guide nucleic acids (gNA), and optionally donor template nucleic acids useful in the modification of a SOD1 gene. The systems are also useful for introduction into cells, for example eukaryotic cells having mutations in the SOD1 protein or the SOD1 regulatory element. Also provided are methods of using such CasX:gNA systems to modify cells having such mutations and utility in methods of treatment of a subject with a SOD1-related disease.

Abstract: Provided herein are engineered proteins comprising a RuvC DNA cleavage domain comprising one or more amino acid modifications, and one or more improved characteristics, relative to a naturally occurring RuvC domain. Also provided are gene editing systems comprising engineered proteins, and methods for use thereof.

Abstract: Provided herein are Class 2 Type V CRISPR:gNA systems comprising Class 2 Type V CRISPR polypeptides (e.g. CasX), guide nucleic acids (gNA), and optionally donor template nucleic acids useful in the modification of a RHO gene. The systems are also useful for introduction into cells, for example eukaryotic cells having mutations in the rhodopsin protein. Also provided are methods of using such systems to modify cells having such mutations and utility in methods of treatment of a subject with a RHO-related disease, such as retinitis pigmentosa.

Abstract: A retractor system includes one or more blades, a handle, and a frame. The handle includes first and second arms with jaw portions and recesses configured to removably retain a blade. Both arms include rotatable connections and handles, where the rotatable connections are between jaw portions and handles. The rotatable connections are connected together such that the arms are rotatable with respect to one another. In one embodiment, the recesses are partial disc shapes opposed to one another, such that when the jaw portions are in a closed position, a disc-shaped space is present between the jaw portions in the closed position. The blade includes a corresponding disc-shape protrusion that may be retained in the recesses, and in some embodiments may include one or more notches to mate with a protrusion in a recess that prevents blade rotation while in the handle.

Abstract: A system and method for analyzing bio-fluid constituents and properties in a bio-fluid sample, wherein the system comprises a bio-fluid collection apparatus, a bio-fluid testing/analysis apparatus, and a reporting apparatus.

Abstract: According to example embodiments, there is provided a handheld mobile computing device, for example a smartphone, includes a computing system, a motion detector, a global positioning system receiver, and a touch-screen display to display a user interface and detect user selections from the user interface. A usage goal-setting user interface includes a dashboard and a plurality of user-selectable menu options, the usage goal-setting user interface including display elements allowing a user to enter one or more parameters respecting the goals including physical activity usage goals, social activity usage goals, educational usage goals, and mindfulness usage goals.

Abstract: A greeting card having a mobile object releasably attached thereto. The mobile object may be shaped like a character or object with one or more wheels at a bottom surface thereof. The mobile object may be removed from the greeting card and set upright onto a surface. Activating a motor module within the mobile object causes the one or more wheels to rotate, setting the mobile object rolling along said surface.

Abstract: Interactive greeting card and gift card holders with a hinged mechanism which provide fun user interaction and triggers audio playback. Each greeting card and gift card holder includes portion thereof which hinges or pivots about an axis. When the greeting card or gift card holder is hinged, or pivoted about the hinge, one or more special effects are triggered. Special effects may include audio, emergence or ejection of a gift card holder (w/gift card) or gift card, the appearance of a thought or word bubble, or any other special effect.

Abstract: Systems and methods for computer screen recording are described herein. In an embodiment, a web-based application includes a screen recorder, a video transcoder, and a video upload manager. The screen recorder records video tutorials, the video transcoder transcodes the recorded video tutorials, and the upload manager uploads the transcoded video tutorial to a server. The server analyzes the uploaded video tutorial to determine search terms that are relevant to the uploaded video tutorial, and associates the determined search terms with the uploaded video tutorial. The server also receives from users search queries containing search terms, searches for video tutorials related to the search terms, and returns a search query result containing links to the related video tutorials.

Abstract: Interactive greeting card and gift card holders with a hinged mechanism which provide fun user interaction and triggers audio playback. Each greeting card and gift card holder includes portion thereof which hinges or pivots about an axis. When the greeting card or gift card holder is hinged, or pivoted about the hinge, one or more special effects are triggered. Special effects may include audio, emergence or ejection of a gift card holder (w/gift card) or gift card, the appearance of a thought or word bubble, or any other special effect.

Abstract: Interactive greeting card and gift card holders with a hinged mechanism which provide fun user interaction and triggers audio playback. Each greeting card and gift card holder includes portion thereof which hinges or pivots about an axis. When the greeting card or gift card holder is hinged, or pivoted about the hinge, one or more special effects are triggered. Special effects may include audio, emergence or ejection of a gift card holder (w/gift card) or gift card, the appearance of a thought or word bubble, or any other special effect.

Abstract: An interactive greeting card with a multi-panel insert which can be unfurled over a portion of the greeting card. In one embodiment, there is a photo or drawing of a character or animal printed on the front cover. A tab is located to the left of and behind the photo or drawing. When a user grasps and moves the tab, a multi-panel paper construct unfurls around the photo or drawing and appears as a wig atop the head of the character or animal depicted in the photo or drawing. Audio replay may also triggered by moving the tab from its original position. In another embodiment, the panels of the multi-panel paper construct are each shaped like a different item or object which is complementary to the theme of the greeting card. Unfolding the multi-panel construct along an outside edge of the greeting card may initiate audio playback and may also activate a motor module which causes movement to at least one mobile object contained on the greeting card.

Abstract: The greeting card of the present invention is a traditional greeting card enhanced by audio and movement. The greeting card contains a pull trigger mechanism so that the user must interact with the greeting card to trigger the audio and motor movement. The pull trigger mechanism may be made of a variety of different materials and are intended to resemble the tail of various animals. Once the pull mechanism or tail has been pulled, the motor is activated causing movement of a portion of the greeting card proximate to the pull mechanism or tail which resembles the behind of an animal. The moveable portion of the greeting card moves, by for example back and forth, while audio plays in the background.