Abstract: Compositions, methods and kits are provided that enable the detection, analysis and/or sequencing of small or large target RNA molecules whether synthetic, purified or within a biological fluid, or in cell lysate that may contain non-target RNA and other contaminating molecules without the need for depletion or purification steps that diminish what might already be low concentrations of the target molecule. The methods, compositions and kits rely on the use of a Group II Intron reverse transcriptase (Intron-RT) that have strand displacing properties and can generate concatemers in cDNA by rolling circle transcription of circRNAs that may be naturally circular or circularized in vitro from linear RNA.

Abstract: Compositions are provided for 3? adapters and methods of use are provided that include methods requiring a plurality of ligation steps involving a single-stranded target polynucleotide and 3? and 5? adapters. Embodiments of the 3? adapters comprise a cleavable linker positioned between a single-stranded region and a double-stranded region. Upon ligating the 3? adapters, the single-stranded region is released by cleaving the cleavable linker.

Abstract: Compositions and methods of use are provided that among other things, allow for efficient adapter ligation to small RNAs. Embodiments of the compositions include partially double stranded polynucleotides for use as 3? adapters that contain a cleavable linker positioned between a single-stranded region and a double-stranded region. Upon ligating the 3? adapters, the single-stranded region is released by cleaving the cleavable linker.

Abstract: Provided herein is a method for chemically capping polynucleotides having a 5? monophosphate. In some embodiments the method may comprise: combining an activated nucleoside 5? mono- or poly-phosphate with a population of polynucleotides that comprises polynucleotides having a 5? monophosphate, to produce a reaction mix; and incubating the reaction mix to produce reaction products that comprise a polynucleotide and a 5? nucleoside cap, linked by a 5? to 5? polyphosphate linkage. The chemical capping method described herein can be incorporated into a variety of cDNA synthesis methods.

Abstract: A computer implemented method (400) of determining a location of one or more transitions (5a-e) on a map and/or the time at which one or more transitions (5a-e) occurs, the one or more transitions (5a-e) made by a set of mobile computing devices (13a-c), from a first zone (2) to a second zone (4), the method (400) comprising: obtaining (402) trajectory data representing a plurality of trajectories (302 a, b) collected from one or more mobile computing devices (13 a-c), at least some of the trajectories (302 a, b) passing through the first zone (2) and/or the second zone (4); positioning (404) the trajectories (302 a, b) in a frame of reference defined relative to the map, wherein at least part of at least some of the trajectories (302 a, b) are positioned based on correspondence with other trajectories (302 a, b); and processing the plurality of trajectories (302 a, b) positioned in the frame of reference defined relative to the map to determine the location and/or time.

Abstract: Compositions are provided for 3? adapters and methods of use are provided that include methods requiring a plurality of ligation steps involving a single-stranded target polynucleotide and 3? and 5? adapters. Embodiments of the 3? adapters comprise a cleavable linker positioned between a single-stranded region and a double-stranded region. Upon ligating the 3? adapters, the single-stranded region is released by cleaving the cleavable linker.

Abstract: Systems, methodologies and components enable the ability to assign, manage and use intermodal, bi-modal and tractor/trailer asset associations between shipping assets, for example, container/truck associations, chassis/container associations, chassis/truck associations, trailer/truck associations, associations for gensets, and refrigerated containers, etc.

Abstract: Systems, methodologies and components enable the ability to assign, manage and use intermodal, bi-modal and tractor/trailer asset associations between shipping assets, for example, container/truck associations, chassis/container associations, chassis/truck associations, trailer/truck associations, associations for gensets, and refrigerated containers, etc.

Abstract: A method of collecting data from (and sending instructions and data to) a reefer is provided, the method includes the steps of connecting a monitoring device on a reefer at an existing connection port for collecting (and delivering) data related to the reefer and wirelessly transmitting the data. A software management system for collecting and monitoring data of a reefer is also provided, the software management system has a monitoring device for obtaining data from a reefer at an connection port of the reefer and wirelessly transmitting the data, and a software system for receiving the data and monitoring and controlling the status of the reefer based on the data received.

Abstract: A method of collecting data from (and sending instructions and data to) a reefer is provided, the method includes the steps of connecting a monitoring device on a reefer at an existing connection port for collecting (and delivering) data related to the reefer and wirelessly transmitting the data. A software management system for collecting and monitoring data of a reefer is also provided, the software management system has a monitoring device for obtaining data from a reefer at an connection port of the reefer and wirelessly transmitting the data, and a software system for receiving the data and monitoring and controlling the status of the reefer based on the data received.

Abstract: A tubular connector body (10) has one end (11) for receiving an optical fibre cable (1) having a plastics outer sleeve (3) protecting an optical fibre (2) and carries at the other end (12) connection means (20) for coupling to another similar connection body. A ferrule (30) is mounted within the other end (12) of the connector body (10) to support an exposed end portion (2a) of the optical fibre (2) within an irradiation settable adhesive material (40) injected into the ferrule (30). A deformable gripping member (50) is inserted into the one end (11) of the connector body (10) to grip an end portion (3a) of the plastics outer sleeve (3) of the cable upon deformation of the gripping member (50) by application of a force to the one end (11) of the tubular connector body (10).