Abstract: A method and system for sterilizing an item using ozone and a secondary molecule is provided. The secondary molecule comprises hydrogen for conversion to a hydroxyl radical. The item and secondary molecular are inserted into a container, and a gaseous mixture containing ozone can be inserted into the container by way of a filling station having an ozone source and a vacuum pump to remove air from the container. Sterilization occurs within the container such that the container can be removed from the filling station. A preferred secondary molecule is an alcohol, such as isopropyl alcohol. Prior to insertion of the item into the container, the item can be soaked in a nutrient rich hot water bath at or near boiling and optionally at a pressure above atmospheric to assist in sterilization.