Abstract: Provided herein are approaches for the detection, identification, and/or selective amplification of specific target species or target variants of nucleic acid sequences, even within an excess of unwanted similar sequences or variants. These approaches include methods, assays, and kits for suppression PCR that require, in part, DNA polymerase that lacks 5?-3? exonuclease activity, and a PCR primer, termed a forward selective primer or a nunchaku primer. The methods, assays, and kits provided herein are useful for a wide variety of applications, including cancer screening assays and kits, personalized screening assays, SNP (single nucleotide polymorphism) genotyping and identification, and downstream applications such as next generation high-throughput genomic sequencing and library construction.

Abstract: Provided herein are approaches for the detection, identification, and/or selective amplification of specific target species or target variants of nucleic acid sequences, even within an excess of unwanted similar sequences or variants. These approaches include methods, assays, and kits for suppression PCR that require, in part, DNA polymerase that lacks 5?-3? exonuclease activity, and a PCR primer, termed a forward selective primer or a nunchaku primer. The methods, assays, and kits provided herein are useful for a wide variety of applications, including cancer screening assays and kits, personalized screening assays, SNP (single nucleotide polymorphism) genotyping and identification, and downstream applications such as next generation high-throughput genomic sequencing and library construction.

Abstract: A method for providing real estate data on an interactive map which provides an interactive map display over which various real estate data layers may be overlaid to provide information regarding displayed property parcels. The present invention generally includes a central communication unit and a user device communicatively interconnected with the central communication unit via a communications network. The central communication unit is adapted to periodically access real estate data from one or more data providers to maintain a data repository containing real estate data regarding a plurality of properties. The user device utilizes a software application to retrieve such real estate data from the central communication unit and display a user interface having a map showing a plurality of map tiles. A data layer may be overlaid over the map to provide the real estate data in an easy-to-read manner while exploring a geographic area on the map.

Abstract: A collectible with a core, a filler material, a fabric surface layer, and a fabric attachment. The core is situated inside the fabric surface layer and the fabric surface layer is stuffed with the filler material to form a body. The fabric attachment is attached to the body to form a body part shape. The collectible has an overall shape, which is substantially like an animal. Also, a method of making a collectible with a sizing step, a placing step, a stuffing step, and an attaching step. The collectible may be made from towels or the like. The core may be a bean bag.

Abstract: A collectible with a core, a filler material, a fabric surface layer, and a fabric attachment. The core is situated inside the fabric surface layer and the fabric surface layer is stuffed with the filler material to form a body. The fabric attachment is attached to the body to form a body part shape. The collectible has an overall shape, which is substantially like an animal. Also, a method of making a collectible with a sizing step, a placing step, a stuffing step, and an attaching step. The collectible may be made from towels or the like. The core may be a bean bag.