Abstract: The invention provides microbial strains possessing improved properties for production of aspartate-derived amino acids and chemicals. Methods of making such strains are provided. These methods include altering expression of the aceBAK operon, the glcB gene, or both. Alteration of expression may be accomplished through increased transcription, relief from native transcriptional control, and/or other means. Replacement of native promoters for these genes is also contemplated; for instance, their native promoters may be replaced by the tac promoter (Ptac).

Abstract: The invention relates to novel bacterial strains and constructs as well as methods for production of L-amino acids, including but not limited to L-threonine. Such novel bacterial strains may be characterized by, for instance, Escherichia coli strains in which an aspartate semialdehyde dehydrogenase (asd) gene is operably associated with at least one non-native promoter, non-native ribosome binding site, or both.

Abstract: The invention relates to novel bacterial strains and constructs as well as methods for production of L-amino acids, including but not limited to L-threonine. Such novel bacterial strains may be characterized by, for instance, Escherichia coli strains in which an aspartate semialdehyde dehydrogenase (asd) gene is operably associated with at least one non-native promoter, non-native ribosome binding site, or both.