Patents by Inventor Sean W. York
Sean W. York has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20150299670Abstract: Four genetic traits have been identified that increase furfural tolerance in microorganisms, such as ethanol-producing Escherichia coli LY180 (strain W derivative). Increased expression of fucO, ucpA or pntAB, and deletion of yqhD were associated with the increase in furfural tolerance. Microorganisms engineered for resistance to furfural were also more resistant to the mixture of inhibitors in hemicellulose hydrolysates, confirming the importance of furfural as an inhibitory component. The combinations of genetic traits disclosed in this application can be applied, generally, to other microorganisms, such as Gram negative and Gram positive bacterial cells, yeast and fungi to increase furfural tolerance in microorganisms used to make industrially useful products.Type: ApplicationFiled: November 14, 2013Publication date: October 22, 2015Inventors: XUAN WANG, LORRAINE P. YOMANO, JAMES Y. LEE, SEAN W. YORK, HUABAO ZHENG, MICHAEL TODD MULLINNIX, KEELNATHAM T. SHANMUGAM, LONNIE O'NEAL INGRAM
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Patent number: 9150887Abstract: The invention relates to bacterium that have increased resistance to furfural and methods of preparation. The invention also relates to methods of producing ethanol using the bacterium and corresponding kits.Type: GrantFiled: January 4, 2010Date of Patent: October 6, 2015Assignee: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.Inventors: Elliot Norman Miller, Laura R. Jarboe, Lorraine P. Yomano, Sean W. York, Keelnatham Shanmugam, Lonnie O'Neal Ingram
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Publication number: 20140141493Abstract: Disclosed are recombinant host cells suitable for degrading an oligosaccharide that have been optimized for growth and production of high yields of ethanol, and methods of making and using these cells. The invention further provides minimal media comprising urea-like compounds for economical production of ethanol by recombinant microorganisms. Recombinant host cells in accordance with the invention are modified by gene mutation to eliminate genes responsible for the production of unwanted products other than ethanol, thereby increasing the yield of ethanol produced from the oligosaccharides, relative to unmutated parent strains. The new and improved strains of recombinant bacteria are capable of superior ethanol productivity and yield when grown under conditions suitable for fermentation in minimal growth media containing inexpensive reagents. Systems optimized for ethanol production combine a selected optimized minimal medium with a recombinant host cell optimized for use in the selected medium.Type: ApplicationFiled: January 6, 2014Publication date: May 22, 2014Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.Inventors: Lonnie O' Neal Ingram, Lorraine P. Yomano, Sean W. York, Brent E. Wood
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Patent number: 8716002Abstract: The invention provides recombinant bacteria, which comprise a full complement of heterologous ethanol production genes. Expression of the full complement of heterologous ethanol production genes causes the recombinant bacteria to produce ethanol as the primary fermentation product when grown in mineral salts medium, without the addition of complex nutrients. Methods for producing the recombinant bacteria and methods for producing ethanol using the recombinant bacteria are also disclosed.Type: GrantFiled: August 8, 2007Date of Patent: May 6, 2014Assignee: University of Florida Research Foundation, Inc.Inventors: Lorraine P. Yomano, Sean W. York, Shengde Zhou, Keelnatham Shanmugam, Lonnie O. Ingram
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Patent number: 8652817Abstract: Disclosed are recombinant host cells suitable for degrading an oligosaccharide that have been optimized for growth and production of high yields of ethanol, and methods of making and using these cells. The invention further provides minimal media comprising urea-like compounds for economical production of ethanol by recombinant microorganisms. Recombinant host cells in accordance with the invention are modified by gene mutation to eliminate genes responsible for the production of unwanted products other than ethanol, thereby increasing the yield of ethanol produced from the oligosaccharides, relative to unmutated parent strains. The new and improved strains of recombinant bacteria are capable of superior ethanol productivity and yield when grown under conditions suitable for fermentation in minimal growth media containing inexpensive reagents. Systems optimized for ethanol production combine a selected optimized minimal medium with a recombinant host cell optimized for use in the selected medium.Type: GrantFiled: June 30, 2006Date of Patent: February 18, 2014Assignee: Univeristy of Florida Research Foundation, Inc.Inventors: Brent E. Wood, Lonnie O. Ingram, Lorraine P. Yomano, Sean W. York
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Publication number: 20120077241Abstract: The invention relates to bacterium that have increased resistance to furfural and methods of preparation. The invention also relates to methods of producing ethanol using the bacterium and corresponding kits.Type: ApplicationFiled: January 4, 2010Publication date: March 29, 2012Applicant: University of Florida Research Foundation, Inc.Inventors: Elliot Norman Miller, Laura R. Jarboe, Lorraine P. Yomano, Sean W. York, Keelnatham Shanmugam, Lonnie O'Neil Ingram
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Publication number: 20100196978Abstract: Disclosed are recombinant host cells suitable for degrading an oligosaccharide that have been optimized for growth and production of high yields of ethanol, and methods of making and using these cells. The invention further provides minimal media comprising urea-like compounds for economical production of ethanol by recombinant microorganisms. Recombinant host cells in accordance with the invention are modified by gene mutation to eliminate genes responsible for the production of unwanted products other than ethanol, thereby increasing the yield of ethanol produced from the oligosaccharides, relative to unmutated parent strains. The new and improved strains of recombinant bacteria are capable of superior ethanol productivity and yield when grown under conditions suitable for fermentation in minimal growth media containing inexpensive reagents. Systems optimized for ethanol production combine a selected optimized minimal medium with a recombinant host cell optimized for use in the selected medium.Type: ApplicationFiled: June 30, 2006Publication date: August 5, 2010Applicant: The University of Florida Research Foundation, IncInventors: Brent E. Wood, Lonnie O. Ingram, Lorraine P. Yomano, Sean W. York
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Publication number: 20100112656Abstract: The invention provides recombinant bacteria, which comprise a full complement of heterologous ethanol production genes. Expression of the full complement of heterologous ethanol production genes causes the recombinant bacteria to produce ethanol as the primary fermentation product when grown in mineral salts medium, without the addition of complex nutrients. Methods for producing the recombinant bacteria and methods for producing ethanol using the recombinant bacteria are also disclosed.Type: ApplicationFiled: August 8, 2007Publication date: May 6, 2010Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.Inventors: Lorraine P. Yomano, Sean W. York, Shengde Zhou, Keelnatham Shanmugam, Lonnie O. Ingram
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Publication number: 20020160469Abstract: This invention presents a method for the production of ethanol that utilizes a soy hydrolysate-based nutrient medium or a yeast autolysate-based medium nutrient medium in conjunction with ethanologenic bacteria and a fermentable sugar for the cost-effective production of ethanol from lignocellulosic biomass. The invention offers several advantages over presently available media for use in ethanol production, including consistent quality, lack of toxins and wide availability.Type: ApplicationFiled: December 17, 2001Publication date: October 31, 2002Applicant: University of Florida Research Foundation, Inc.Inventors: Lonnie O. Ingram, Sean W. York
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Patent number: 6102690Abstract: This invention relates to a recombinant microorganism which expresses pyruvate decarboxylase, alcohol dehydrogenase, Klebsiella phospho-.beta.-glucosidase and Klebsiella (phosphoenolpyruvate-dependent phosphotransferase system) cellobiose-utilizing Enzyme II, wherein said phospho-.beta.-glucosidase and said (phosphoenolpyruvate-dependent phosphotransferase) cellobiose-utilizing Enzyme II are heterologous to said microorganism and wherein said microorganism is capable of utilizing both hemicellulose and cellulose, including cellobiose, in the production of ethanol.Type: GrantFiled: April 7, 1997Date of Patent: August 15, 2000Assignee: Univ. of Florida Research Foundation, Inc.Inventors: Lonnie O. Ingram, Xiaokuang Lai, Mohammed Moniruzzaman, Sean W. York