Patents by Inventor Sebastiaan Johannes Trietsch
Sebastiaan Johannes Trietsch has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230250377Abstract: A microfluidic cell culture device is described. The device comprises a microfluidic network comprising a base, a microfluidic channel, and a cover, and at least one perfusion compartment and at least one support compartment inside the microfluidic channel The base and cover each comprise an aperture, thereby defining a conduit through the microfluidic channel. The aperture is in fluidic contact with the at least one support compartment, and with the at least one perfusion compartment through the at least one support compartment. Methods for creating a fluid-fluid interface and for investigating a cellular response to a stimulant using the device are also described.Type: ApplicationFiled: July 9, 2021Publication date: August 10, 2023Inventors: Sebastiaan Johannes Trietsch, Arnaud Yannick Michel Nicolas, Leonarda Jacoba Van Den Broek, Bart Kramer
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Patent number: 11662342Abstract: The current invention relates to an improved method for measuring modulating effects of compounds on epithelial cell barrier function. The methods allows for high throughput screening of test compounds individually or in combination. Compounds improving the epithelial barrier function or compounds negatively influencing epithelial barrier function can be analyzed with the method described herein.Type: GrantFiled: July 8, 2016Date of Patent: May 30, 2023Assignee: MIMETAS B.V.Inventors: Paul Vulto, Sebastiaan Johannes Trietsch, Henriëtte Leonore Lanz, Marianne Katharina Vormann
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Patent number: 11629319Abstract: A method of vascularising a cell aggregate on a microfluidic device, microfluidic cell culture devices comprising perfusable vascular networks and kits and assays using the microfluidic cell culture devices are described. The microfluidic devices comprise one or more capillary pressure barriers allowing for formation of an extracellular matrix gel within a confined area of the network, in which cells can be cultured for different uses.Type: GrantFiled: June 12, 2017Date of Patent: April 18, 2023Assignee: Mimetas, B.V.Inventors: Paul Vulto, Sebastiaan Johannes Trietsch, Arnaud Nicolas
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Publication number: 20220395833Abstract: The present invention relates to a microfluidic cell culture system comprising at least one microfluidic structure, wherein the at least one microfluidic structure comprises a cell culture chamber, a first and second reservoir in fluid communication with each other via the cell culture chamber, wherein the microfluidic cell culture system further comprises a detachable seal for sealing the at least one microfluidic structure and wherein the microfluidic cell culture system is configured such that the first and second reservoir of the at least one microfluidic structure are in fluid communication with each other via a communication channel that does not comprise the cell culture chamber.Type: ApplicationFiled: November 9, 2020Publication date: December 15, 2022Inventors: Kristina BISHARD, Julia GRASEGGER, Sebastiaan Johannes TRIETSCH
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Patent number: 11344877Abstract: The present invention relates to an apparatus for controlling the shape and/or position of a moveable fluid-fluid meniscus, and methods of use, in particular a method to control the shape of a moveable fluid-fluid meniscus in an apparatus in which the meniscus is caused to align along a stable capillary barrier or phaseguide.Type: GrantFiled: September 10, 2013Date of Patent: May 31, 2022Assignee: UNIVERSITEIT LEIDENInventors: Paul Vulto, Sebastiaan Johannes Trietsch, Ender Yildirim
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Patent number: 10900008Abstract: Apparatus for processing life-based organic particles, including particles selected from the list comprising cells, cellular spheroids, tissues, eukaryotes, micro-organisms, organs or embryos, comprises a hollow volume (10) that (a) is internally divided into at least first (14), second (16) and third (17) sub-volumes by at least two phaseguides (12, 13) formed inside the volume and (b) includes parts that are relatively upstream and relatively downstream when judged with reference to the movement of a meniscus or a bulk liquid in the volume (10). The apparatus includes at least first, second and third fluid conduits (19, 21, 22) connected to permit fluid communication between the upstream exterior of the volume (10) and a respective said sub-volume (14, 16, 17); and at least one further conduit (24) connected to permit fluid communication between the downstream exterior of the volume (10) and a said sub-volume.Type: GrantFiled: September 16, 2019Date of Patent: January 26, 2021Assignee: UNIVERSITEIT LEIDENInventors: Paul Vulto, Sebastiaan Johannes Trietsch, Heiko Jan van der Linden, Adrianus Theodoras Joore, Thomas Hankemeier
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Publication number: 20200071650Abstract: Apparatus for processing life-based organic particles, including particles selected from the list comprising cells, cellular spheroids, tissues, eukaryotes, micro-organisms, organs or embryos, comprises a hollow volume (10) that (a) is internally divided into at least first (14), second (16) and third (17) sub-volumes by at least two phaseguides (12, 13) formed inside the volume and (b) includes parts that are relatively upstream and relatively downstream when judged with reference to the movement of a meniscus or a bulk liquid in the volume (10). The apparatus includes at least first, second and third fluid conduits (19, 21, 22) connected to permit fluid communication between the upstream exterior of the volume (10) and a respective said sub-volume (14, 16, 17); and at least one further conduit (24) connected to permit fluid communication between the downstream exterior of the volume (10) and a said sub-volume.Type: ApplicationFiled: September 16, 2019Publication date: March 5, 2020Applicant: UNIVERSITEIT LEIDENInventors: Paul Vulto, Sebastiaan Johannes Trietsch, Heiko Jan van der Linden, Adrianus Theodorus Joore, Thomas Hankemeier
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Publication number: 20200063081Abstract: A method of vascularising a cell aggregate on a microfluidic device, microfluidic cell culture devices comprising perfusable vascular networks and kits and assays using the microfluidic cell culture devices are described. The microfluidic devices comprise one or more capillary pressure barriers allowing for formation of an extracellular matrix gel within a confined area of the network, in which cells can be cultured for different uses.Type: ApplicationFiled: June 12, 2017Publication date: February 27, 2020Applicant: Mimetas B.V.Inventors: Paul VULTO, Sebastiaan Johannes TRIETSCH, Arnaud NICOLAS
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Patent number: 10532355Abstract: Microfluidic plate comprising a plurality of microfluidic networks and inlets providing access to the microfluidic networks, wherein each microfluidic network comprises a capillary pressure barrier and extends through a first plane, each inlet is formed by an inlet chamber having a bottom surface, a support structure is provided above each inlet, which support structure defines an opening and comprises at least one support member positioned at the opening such that a circle can be defined in a second plane extending parallel to the first plane, which circle has the largest possible diameter while being completely located within the opening and in contact with the at least one support member, and the support structure is configured such that a first empty space is formed, which first empty space extends from the second plane towards the inlet chamber and has the form of a right circular cone or a truncated right circle cone, wherein the circle forms a base plane of the right circular cone or the truncated righType: GrantFiled: June 5, 2015Date of Patent: January 14, 2020Assignee: Mimetas B.V.Inventors: Sebastiaan Johannes Trietsch, Paul Vulto
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Patent number: 10450542Abstract: Apparatus for processing life-based organic particles, including particles selected from the list comprising cells, cellular spheroids, tissues, eukaryotes, micro-organisms, organs or embryos, comprises a hollow volume (10) that (a) is internally divided into at least first (14), second (16) and third (17) sub-volumes by at least two phaseguides (12, 13) formed inside the volume and (b) includes parts that are relatively upstream and relatively downstream when judged with reference to the movement of a meniscus or a bulk liquid in the volume (10). The apparatus includes at least first, second and third fluid conduits (19, 21, 22) connected to permit fluid communication between the upstream exterior of the volume (10) and a respective said sub-volume (14, 16, 17); and at least one further conduit (24) connected to permit fluid communication between the downstream exterior of the volume (10) and a said sub-volume.Type: GrantFiled: March 8, 2012Date of Patent: October 22, 2019Assignee: UNIVERSITEIT LEIDENInventors: Paul Vulto, Sebastiaan Johannes Trietsch, Heiko Jan van der Linden, Adrianus Theodorus Joore, Thomas Hankemeier
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Publication number: 20190076842Abstract: The present invention relates to a method of culturing and/or monitoring epithelial cells using a microfluidic cell culture system comprising a microfluidic channel network. In the method epithelial cells are lined, in the microfluidic cell culture system by cells of mesenchymal origin. The cells may form a tubular or tube-like structure, i.e. a.tube in a tube. The method allows for improved epithelial models suitable for a wide variety of applications, including but not limited to high-throughput screening and analysis of epithelium in health and disease.Type: ApplicationFiled: March 9, 2017Publication date: March 14, 2019Inventors: Paul VULTO, Dorota Malgorzata KUREK, Adrianus Theodorus JOORE, Sebastiaan Johannes TRIETSCH, Henriëtte Leonore LANZ
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Publication number: 20180345280Abstract: The invention relates to an apparatus for inducing flow of a fluid in a microfluidic device that comprises at least one microfluidic channel, the apparatus comprising: a base, on which said microfluidic device is pivotally disposed, defining a first position in a first plane (I); and a selectively operable tilting element attached to said base, to pivot the microfluidic device with respect to the base, thereby inducing fluid flow through the at least one microfluidic channel; wherein the base accommodates the microfluidic device in a first position defining a first plane, wherein the tilting element pivots the microfluidic device from the first position to a second position defining a second plane (II), and wherein the microfluidic device is tilted about a pivoting axis bisecting the first plane, thereby defining an inclination angle ? between the first plane and second plane.Type: ApplicationFiled: November 28, 2016Publication date: December 6, 2018Applicant: MIMETAS B.V.Inventors: Paul VULTO, Sebastiaan Johannes TRIETSCH, Arnaud Yannick Michel NICOLAS
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Publication number: 20180196035Abstract: The current invention relates to an improved method for measuring modulating effects of compounds on epithelial cell barrier function. The methods allows for high throughput screening of test compounds individually or in combination. Compounds improving the epithelial barrier function or compounds negatively influencing epithelial barrier function can be analyzed with the method described herein.Type: ApplicationFiled: July 8, 2016Publication date: July 12, 2018Inventors: Paul VULTO, Sebastiaan Johannes TRIETSCH, Henriëtte Leonore LANZ, Marianne Katharina VORMANN
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Patent number: 9771553Abstract: Apparatus for processing liquids or liquid-based substances includes a plurality of volumes at least two of which are defined at least in part by one or more phaseguides inside the volume and/or in a conduit connected thereto for controlling aliquoting of one or more liquids or liquid-based substances inside the volume. Each volume has an upstream and downstream side with respect to meniscus advancement direction via which it may be filled with or emptied of one or more liquids or liquid-based substances. The apparatus also includes at least one common upstream-side conduit connected to supply a liquid or liquid-based substance via a plurality of the inlet or extraction conduits, a plurality of the phaseguides exhibiting a predetermined level of stability and one or more of the phaseguides exhibiting a predetermined different stability compared with the stability of at least one of the other phaseguides whereby to control the preference order in which the volumes fill and/or empty.Type: GrantFiled: March 8, 2012Date of Patent: September 26, 2017Assignee: UNIVERSITEIT LEIDENInventors: Paul Vulto, Sebastiaan Johannes Trietsch, Heiko Jan van der Linden, Thomas Hankemeier
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Patent number: 9429249Abstract: The present invention relates to a fluid triggerable passive valve for controlling flow of one or more fluids comprising: a volume comprising at least two capillary pressure barriers for respectively pinning a first and second fluid-fluid meniscus, the two capillary pressure barriers dividing the volume in at least three sub volumes, whereby two sub volumes being positioned upstream and one sub volume being positioned downstream with respect to the advancement direction of each respective fluid-fluid meniscus; wherein the capillary pressure barriers are spaced apart such that upon pinning of a first fluid-fluid meniscus on a first capillary pressure barrier and arrival or pinning of a second fluid-fluid meniscus on or at a second capillary pressure barrier, the two menisci touch and thereby coalesce into one fluid-fluid meniscus.Type: GrantFiled: August 21, 2014Date of Patent: August 30, 2016Assignee: Universiteit LeidenInventors: Paul Vulto, Sebastiaan Johannes Trietsch, Ender Yildirim
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Publication number: 20150238952Abstract: The present invention relates to an apparatus for controlling the shape and/or position of a moveable fluid-fluid meniscus, the apparatus comprising a volume for containing and directing fluid, the filling direction being a downstream direction, including the meniscus and the volume having at least a first structure defining a capillary pressure barrier along which the meniscus tends to align, the capillary pressure barrier and the meniscus defining a boundary in the volume between at least two sub-volumes,wherein (a) the capillary pressure barrier is stabilized by subtending at both ends an angle with a wall of the volume that on the downstream side of the capillary pressure barrier is greater than 90°, while not providing a deliberate fluid alignment weakness along the capillary pressure barrier that reduces the stability of the capillary pressure barrier and/or (b) wherein the capillary pressure is stabilized by providing a stretching barrier at a distance less than the maximum stretching distance of the fType: ApplicationFiled: September 10, 2013Publication date: August 27, 2015Applicant: UNIVERSITEIT LEIDENInventors: Paul Vulto, Sebastiaan Johannes Trietsch, Ender Yildirim
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Publication number: 20150040999Abstract: The present invention relates to a fluid triggerable passive valve for controlling flow of one or more fluids comprising: a volume comprising at least two capillary pressure barriers for respectively pinning a first and second fluid-fluid meniscus, the two capillary pressure barriers dividing the volume in at least three sub volumes, whereby two sub volumes being positioned upstream and one sub volume being positioned downstream with respect to the advancement direction of each respective fluid-fluid meniscus; wherein the capillary pressure barriers are spaced apart such that upon pinning of a first fluid-fluid meniscus on a first capillary pressure barrier and arrival or pinning of a second fluid-fluid meniscus on or at a second capillary pressure barrier, the two menisci touch and thereby coalesce into one fluid-fluid meniscusType: ApplicationFiled: August 21, 2014Publication date: February 12, 2015Inventors: Paul VULTO, Sebastiaan Johannes Trietsch, Ender Yildirim
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Publication number: 20140065597Abstract: Apparatus for processing life-based organic particles, including particles selected from the list comprising cells, cellular spheroids, tissues, eukaryotes, micro-organisms, organs or embryos, comprises a hollow volume (10) that (a) is internally divided into at least first (14), second (16) and third (17) sub-volumes by at least two phaseguides (12, 13) formed inside the volume and (b) includes parts that are relatively upstream and relatively downstream when judged with reference to the movement of a meniscus or a bulk liquid in the volume (10). The apparatus includes at least first, second and third fluid conduits (19, 21, 22) connected to permit fluid communication between the upstream exterior of the volume (10) and a respective said sub-volume (14, 16, 17); and at least one further conduit (24) connected to permit fluid communication between the downstream exterior of the volume (10) and a said sub-volume.Type: ApplicationFiled: March 8, 2012Publication date: March 6, 2014Applicant: UNIVERSITEIT LEIDENInventors: Paul Vulto, Sebastiaan Johannes Trietsch, Heiko Jan Van der linden, Adrianus Theodorus Joore, Thomas Hankemeier
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Patent number: D898216Type: GrantFiled: May 15, 2019Date of Patent: October 6, 2020Inventors: Arnaud Nicolas, Frederik Schavemaker, Sebastiaan Johannes Trietsch
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Patent number: D970034Type: GrantFiled: May 15, 2019Date of Patent: November 15, 2022Inventors: Frederik Schavemaker, Sebastiaan Johannes Trietsch, Arnaud Nicolas