Patents by Inventor Sebastiaan Menno Bosma

Sebastiaan Menno Bosma has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11667931
    Abstract: The present invention relates to the production of adeno-associated viral vectors in insect cells. The insect cells therefore comprise a first nucleotide sequence encoding the adeno-associated virus (AAV) capsid proteins, whereby the initiation codon for translation of the AAV VP1 capsid protein is an AUG. Upstream of the VP1 open reading frame an alternative out of frame start codon is placed such that translation initiation of the VP1 protein is modified, i.e. reduced, to allow production of VP1:VP2:VP3 in a good stoichiometry resulting in AAV with high potency.
    Type: Grant
    Filed: January 15, 2020
    Date of Patent: June 6, 2023
    Assignee: uniQure IP B.V.
    Inventors: David Johannes Francois Du Plessis, Olivier Ter Brake, Sebastiaan Menno Bosma, Harald Peter Albert Petry, Jacek Lubelski
  • Publication number: 20230159951
    Abstract: The present invention relates novel combinations of nucleic acid constructs for the production of recombinant parvoviral gene therapy vectors. In particular the invention relates a combination preferably no more than two construct, the first construct expressing both the parvoviral Cap and Rep proteins, and the second construct at least comprising the transgene flanked ITRs and optionally again comprising an expression cassette forthe Cap proteins. The nucleic acid constructs are preferably baculoviral vectors for the production of rAAV in insect cells.
    Type: Application
    Filed: September 20, 2022
    Publication date: May 25, 2023
    Inventors: David Johannes Francois DU PLESSIS, ANGGAKUSUMA, Sebastiaan Menno BOSMA, Jacek LUBELSKI
  • Patent number: 10837027
    Abstract: The present invention relates to the production of adeno-associated viral vectors in insect cells. The insect cells therefore comprise a first nucleotide sequence encoding the adeno-associated virus (AAV) capsid proteins, whereby the initiation codon for translation of the AAV VP1 capsid protein is a non-ATG, suboptimal initiation codon and wherein the coding sequence for one or more amino acid residues have been inserted between the suboptimal translation initiation codon and the codon encoding the amino acid residue that corresponds to the amino acid residue at position 2 of the wild type capsid amino acid sequence of which the first amino acid residue is alanine, glycine valine, aspartic acid or glutamic acid.
    Type: Grant
    Filed: March 10, 2015
    Date of Patent: November 17, 2020
    Assignee: UNIQURE IP B.V.
    Inventors: Jacek Lubelski, Sebastiaan Menno Bosma, Harald Peter Albert Petry, Wilhelmus Theodorus Johannes Maria Christiaan Hermens
  • Publication number: 20200248206
    Abstract: The present invention relates to the production of adeno-associated viral vectors in insect cells. The insect cells therefore comprise a first nucleotide sequence encoding the adeno-associated virus (AAV) capsid proteins, whereby the initiation codon for translation of the AAV VP1 capsid protein is an AUG. Upstream of the VP1 open reading frame an alternative out of frame start codon is placed such that translation initiation of the VP1 protein is modified, i.e. reduced, to allow production of VP1:VP2:VP3 in a good stoichiometry resulting in AAV with high potency.
    Type: Application
    Filed: January 15, 2020
    Publication date: August 6, 2020
    Inventors: David Johannes Francois Du Plessis, Olivier Ter Brake, Sebastiaan Menno Bosma, Harald Peter Albert Petry, Jacek Lubelski
  • Publication number: 20170356008
    Abstract: The present invention relates to the production of adeno-associated viral vectors in insect cells. The insect cells therefore comprise a first nucleotide sequence encoding the adeno-associated virus (AAV) capsid proteins, whereby the initiation codon for translation of the AAV VP1 capsid protein is a non-ATG, suboptimal initiation codon and wherein the coding sequence for one or more amino acid residues have been inserted between the suboptimal translation initiation codon and the codon encoding the amino acid residue that corresponds to the amino acid residue at position 2 of the wild type capsid amino acid sequence of which the first amino acid residue is alanine, glycine valine, aspartic acid or glutamic acid.
    Type: Application
    Filed: March 10, 2015
    Publication date: December 14, 2017
    Applicant: UniQure IP B.V.
    Inventors: Jacek LUBELSKI, Sebastiaan Menno BOSMA, Harald Peter Albert PETRY, Wilhelmus Theodorus Johannes Maria Christiaan HERMENS
  • Publication number: 20110171262
    Abstract: Parvoviral capsid with incorporated Gly-Ala repeat region The present invention provides a nucleic acid construct comprising a nucleic acid sequence encoding a parvoviral VP1, VP2 and VP3 capsid proteins comprising an immuno evasion repeat sequence. In addition, the present invention provides a cell comprising such construct, a parvoviral virion comprising a capsid protein that comprises an immune evasion repeat sequence, use of that parvoviral virion in gene therapy and a pharmaceutical composition comprising such parvoviral virion.
    Type: Application
    Filed: June 17, 2009
    Publication date: July 14, 2011
    Inventors: Andrew Christian Bakker, Valerie Sier-Ferreira, Sebastiaan Menno Bosma