Patents by Inventor Sebastian A. Diaz

Sebastian A. Diaz has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20250084459
    Abstract: An energy transfer platform involves a CdSe/ZnS core-shell quantum dot (QD) scaffold having four additional components: terbium metal chelate, ruthenium(II)-phenanthroline, AlexaFluor 647, and Cy5.5. These are positioned in a self-assembled fashion on the QD surface through the utilization of peptide-PNA and DNA bioconjugate linkers. The modular platform can operate to sense multiple different events or targets simultaneously, including sensing of genetic moieties and enzymatic targets.
    Type: Application
    Filed: July 17, 2024
    Publication date: March 13, 2025
    Applicant: The Government of the United States of America, as represented by the Secretary of the Navy
    Inventors: Igor L Medintz, Matthew Chiriboga, Shelby L. Hooe, David A. Hastman, Niko Hildebrandt, Sebastian A. Diaz, Kimihiro Susumu
  • Publication number: 20240344124
    Abstract: One can enrich nucleic acid (RNA or DNA) from within a complex mixture in a sequence-specific manner by using a mutant of a Cas enzyme lacking nuclease activity, for example a mutant of Cas13a (dCas) from Leptotrichia wadeii. The nucleic acid is bound by the mutant protein as directed by guide RNA molecules (crRNA) and purified after association with an appropriate surface, such as quantum dots or functionalized beads. The nucleic acid is separated from the Cas and purified after precipitation or interaction with another surface. The resulting product can be directly processed via long-read sequencing.
    Type: Application
    Filed: November 15, 2023
    Publication date: October 17, 2024
    Inventors: Christopher M. Green, Joseph R. Spangler, Tomasz A. Leski, Kimihiro Susumu, Sebastian A. Diaz, Igor L. Medintz, David A. Stenger, Scott N. Dean
  • Publication number: 20240254531
    Abstract: Enzyme cascade reactions, wherein the product of a first enzyme is the substrate of a second enzyme and so forth, were found to be enhanced by the presence of peptides. It is believed that the peptides operate by forming coacervates, which are membrane-less compartments where, in the case of coacervates formed by peptides, the liquid-liquid phase separation involves water as the continuous phase both inside and outside the coacervate.
    Type: Application
    Filed: January 29, 2024
    Publication date: August 1, 2024
    Inventors: Joyce C. Breger, Kimihiro Susumu, Igor L. Medintz, Gregory A. Ellis, Sebastian A. Diaz, Shelby L. Hooe
  • Publication number: 20240175076
    Abstract: A flexible strategy for assembling luminescent and colorimetric quantum dot/nucleic acid hairpin (QD-HP) molecular beacons for use in CRISPR/Cas diagnostics uses chimeric peptide/peptide nucleic acid (PNA) to conjugate fluorescently labeled DNA or RNA hairpins to ZnS-coated QDs.
    Type: Application
    Filed: July 5, 2023
    Publication date: May 30, 2024
    Inventors: Sebastián A. Díaz, Christopher M. Green, Joseph R. Spangler, Divita Mathur, Kimihiro Susumu, David A. Stenger, Igor L. Medintz
  • Publication number: 20240060118
    Abstract: A FRET based reporter system utilizing the fluorescent semiconductor quantum dots (QDs) and restriction enzymes expressed in cell-free system has the potential to detect multiple analytes in single reaction.
    Type: Application
    Filed: May 30, 2023
    Publication date: February 22, 2024
    Inventors: Scott A. Walper, Igor L. Medintz, Divita Mathur, Sebastian A. Diaz, Meghna Thakur, Kimihiro Susumu, Michael H. Stewart
  • Patent number: 11512305
    Abstract: A nanoparticle (for example, quantum dot) serves as a substrate for immobilizing enzymes involved in consecutive reactions as a cascade. This results in a significant increase in the rate of catalysis as well as final product yield compared to non-immobilized enzymes.
    Type: Grant
    Filed: December 13, 2017
    Date of Patent: November 29, 2022
    Assignee: The Government of the United States of America, as represented by the Secretary of the Navy
    Inventors: Igor L. Medintz, James N. Vranish, Mario Ancona, Kimihiro Susumu, Sebastian A. Diaz
  • Publication number: 20180171325
    Abstract: A nanoparticle (for example, quantum dot) serves as a substrate for immobilizing enzymes involved in consecutive reactions as a cascade. This results in a significant increase in the rate of catalysis as well as final product yield compared to non-immobilized enzymes.
    Type: Application
    Filed: December 13, 2017
    Publication date: June 21, 2018
    Inventors: Igor L. Medintz, James N. Vranish, Mario Ancona, Kimihiro Susumu, Sebastian A. Diaz