Patents by Inventor SEBASTIAN TREUSCH
SEBASTIAN TREUSCH has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11807956Abstract: Disclosed herein are methods for the generation of highly accurate oligonucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a selective library of variants. The variant oligonucleic acid libraries described herein may designed for further processing by transcription or translation. The variant oligonucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease.Type: GrantFiled: October 12, 2020Date of Patent: November 7, 2023Assignee: Twist Bioscience CorporationInventors: Anthony Cox, Sebastian Treusch, Siyuan Chen
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Publication number: 20220315971Abstract: Provided herein are methods, systems, and compositions for seamless nucleic acid assembly. Such methods, systems, and compositions for seamless nucleic acid assembly include those for in vitro recombination cloning, single-stranded hierarchal DNA assembly, or overlap extension PCR without primer removal.Type: ApplicationFiled: May 26, 2022Publication date: October 6, 2022Inventors: Cheng-Hsien WU, Sebastian TREUSCH
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Patent number: 11377676Abstract: Provided herein are methods, systems, and compositions for seamless nucleic acid assembly. Such methods, systems, and compositions for seamless nucleic acid assembly include those for in vitro recombination cloning, single-stranded hierarchal DNA assembly, or overlap extension PCR without primer removal.Type: GrantFiled: December 12, 2019Date of Patent: July 5, 2022Assignee: Twist Bioscience CorporationInventors: Cheng-Hsien Wu, Sebastian Treusch
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Publication number: 20220064628Abstract: Methods and compositions are provided for assembly of large nucleic acids where the assembled large nucleic acids lack internal sequence modifications made during the assembly process.Type: ApplicationFiled: May 13, 2021Publication date: March 3, 2022Inventors: Esteban Toro, Sebastian TREUSCH, Siyuan CHEN, Cheng-Hsien WU
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Publication number: 20210246488Abstract: The disclosure relates to methods and compositions for evaluating emulsion uniformity. In an exemplary embodiment, the disclosure provides a method for evaluating a quality characteristic of a droplet. The method includes the steps of: encapsulating a plurality of polynucleotides in a droplet, wherein the plurality of polynucleotides comprises at least one species of oligonucleotide; tagging the polynucleotides with a label that identifies the polynucleotides as arising from the droplet; counting a number of species of oligonucleotide tagged with the label; and determining a quality characteristic of the droplet based on the number of species of oligonucleotide tagged with the label. The oligonucleotide may include a first nucleic acid segment and a second nucleic acid segment, wherein the first nucleic acid segment comprises a plurality of random nucleotides; and the second nucleic acid segment comprises a conserved region common to the plurality of oligonucleotides.Type: ApplicationFiled: August 14, 2018Publication date: August 12, 2021Applicant: Mission Bio, Inc.Inventors: Sebastian Treusch, Adam R. Sciambi, Maurizio Pellegrino, Dennis Jay Eastburn
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Publication number: 20210040476Abstract: Disclosed herein are methods for the generation of highly accurate oligonucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a selective library of variants. The variant oligonucleic acid libraries described herein may designed for further processing by transcription or translation. The variant oligonucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease.Type: ApplicationFiled: October 12, 2020Publication date: February 11, 2021Inventors: Anthony COX, Sebastian Treusch, Siyuan CHEN
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Patent number: 10844373Abstract: Disclosed herein are methods for the generation of highly accurate oligonucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a selective library of variants. The variant oligonucleic acid libraries described herein may designed for further processing by transcription or translation. The variant oligonucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease.Type: GrantFiled: September 16, 2016Date of Patent: November 24, 2020Assignee: Twist Bioscience CorporationInventors: Anthony Cox, Sebastian Treusch, Siyuan Chen
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Publication number: 20200181667Abstract: Provided herein are methods, systems, and compositions for seamless nucleic acid assembly. Such methods, systems, and compositions for seamless nucleic acid assembly include those for in vitro recombination cloning, single-stranded hierarchal DNA assembly, or overlap extension PCR without primer removal.Type: ApplicationFiled: December 12, 2019Publication date: June 11, 2020Inventors: Cheng-Hsien WU, Sebastian TREUSCH
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Publication number: 20190352635Abstract: Methods and compositions are provided for assembly of large nucleic acids where the assembled large nucleic acids lack internal sequence modifications made during the assembly process.Type: ApplicationFiled: August 2, 2019Publication date: November 21, 2019Inventors: Esteban Toro, Sebastian TREUSCH, Siyuan CHEN, Cheng-Hsien WU
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Publication number: 20190329209Abstract: Disclosed herein are methods and compositions for the emulsification of solid supports in deformable gel beads. The methods and compositions provided herein may be used in microfluidic systems and devices. In some aspects of the disclosure, deformable gel beads containing solid supports may be paired with single cell entities. The methods and compositions provided herein may be suitable for single cell analysis, including, but not limited to, labeling single cells or components thereof for downstream analysis.Type: ApplicationFiled: June 13, 2017Publication date: October 31, 2019Applicant: Mission Bio, Inc.Inventors: Adam R. Sciambi, Dennis Jay Eastburn, Sebastian Treusch
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Patent number: 10240160Abstract: Disclosed are yeast cells expressing a polypeptide comprising a signal sequence and a human amyloid beta protein. Also disclosed are methods of screening yeast cells to identify compounds that prevent or suppress amyloid beta-induced toxicity and genetic suppressors or enhancers of amyloid beta-induced toxicity. Compounds identified by such screens can be used to treat or prevent neurodegenerative disorders such as Alzheimer's disease.Type: GrantFiled: May 4, 2017Date of Patent: March 26, 2019Assignee: Whitehead Institute for Biomedical ResearchInventors: Kent E. S. Matlack, Susan L. Lindquist, Sebastian Treusch
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Publication number: 20180016588Abstract: Disclosed are yeast cells expressing a polypeptide comprising a signal sequence and a human amyloid beta protein. Also disclosed are methods of screening yeast cells to identify compounds that prevent or suppress amyloid beta-induced toxicity and genetic suppressors or enhancers of amyloid beta-induced toxicity. Compounds identified by such screens can be used to treat or prevent neurodegenerative disorders such as Alzheimer's disease.Type: ApplicationFiled: May 4, 2017Publication date: January 18, 2018Applicant: Whitehead Institute for Biomedical ResearchInventors: Kent E.S. Matlack, Susan L. Lindquist, Sebastian Treusch
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Patent number: 9677067Abstract: Methods and compositions are provided for assembly of large nucleic acids where the assembled large nucleic acids lack internal sequence modifications made during the assembly process.Type: GrantFiled: May 13, 2016Date of Patent: June 13, 2017Assignee: TWIST BIOSCIENCE CORPORATIONInventors: Esteban Toro, Sebastian Treusch, Siyuan Chen, Cheng-Hsien Wu
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Patent number: 9677079Abstract: Disclosed are yeast cells expressing a polypeptide comprising a signal sequence and a human amyloid beta protein. Also disclosed are methods of screening yeast cells to identify compounds that prevent or suppress amyloid beta-induced toxicity and genetic suppressors or enhancers of amyloid beta-induced toxicity. Compounds identified by such screens can be used to treat or prevent neurodegenerative disorders such as Alzheimer's disease.Type: GrantFiled: January 11, 2011Date of Patent: June 13, 2017Assignee: Whitehead Insititute for Biomedical ResearchInventors: Kent E. S. Matlack, Susan L. Lindquist, Sebastian Treusch
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Publication number: 20170159044Abstract: Methods and compositions are provided for assembly of large nucleic acids where the assembled large nucleic acids lack internal sequence modifications made during the assembly process.Type: ApplicationFiled: February 15, 2017Publication date: June 8, 2017Inventors: Esteban Toro, Sebastian TREUSCH, Siyuan CHEN, Cheng-Hsien WU
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Publication number: 20170081660Abstract: Disclosed herein are methods for the generation of highly accurate oligonucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a selective library of variants. The variant oligonucleic acid libraries described herein may designed for further processing by transcription or translation. The variant oligonucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease.Type: ApplicationFiled: September 16, 2016Publication date: March 23, 2017Inventors: Anthony COX, Sebastian TREUSCH, Siyuan CHEN
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Publication number: 20160264958Abstract: Methods and compositions are provided for assembly of large nucleic acids where the assembled large nucleic acids lack internal sequence modifications made during the assembly process.Type: ApplicationFiled: May 13, 2016Publication date: September 15, 2016Inventors: Esteban Toro, Sebastian Treusch, Siyuan Chen, Cheng-Hsien Wu
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Publication number: 20130045483Abstract: Disclosed are yeast cells expressing a polypeptide comprising a signal sequence and a human amyloid beta protein. Also disclosed are methods of screening yeast cells to identify compounds that prevent or suppress amyloid beta-induced toxicity and genetic suppressors or enhancers of amyloid beta-induced toxicity. Compounds identified by such screens can be used to treat or prevent neurodegenerative disorders such as Alzheimer's disease.Type: ApplicationFiled: June 29, 2012Publication date: February 21, 2013Applicant: WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCHInventors: SEBASTIAN TREUSCH, KENT E.S. MATLACK, SUSAN L. LINDQUIST