Abstract: A flow cell for a liquid chromatography detector comprises a substrate formed of a glass material; a fluidic channel extending through the substrate; and at least one gas filled region formed in the substrate along at least a portion of a length of the fluidic channel. A portion of the glass material separates the fluidic channel and the gas filled region. An interface between the gas filled region and the portion of the glass material separating the fluidic channel and the gas filled region enables total internal reflection of light propagating along the fluidic channel.

Abstract: Exemplary embodiments are directed to methods and systems for controlling fluid parameters within a detector. A first and second fluid pump manager control the flow of a first and second fluid to one or more heating/cooling devices, and a mixer receives and mixes the first and second fluids. An optical detector flow cell receives the fluid mixture from the mixer, and a pressure regulator controls the pressure at the optical detector flow cell. Thus, the composition, temperature, and pressure of a fluid mixture entering an optical detector flow cell can be controlled in real time.

Abstract: Techniques and apparatus for thermally controlled interfaces for separation devices and optical flow cell devices with minimized post-column volumes are described. In one embodiment, for example, a column-optical cell assembly may include an insulating device, a chromatography column arranged within the insulating device, and an optical flow cell in fluid communication with the chromatography column, the chromatography column arranged within a minimum distance of the optical flow cell.

Abstract: The present disclosure relates to optical flow cells for use in chromatography or extraction systems. The optical flow cells include an inlet portion configured to receive a highly compressible fluid and an inlet transition portion or gasket having an internal volume and internal geometry configured to receive the highly compressible fluid from the inlet portion. The optical flow cell also includes an optical path portion configured to receive the highly compressible fluid from the inlet transition portion and direct the highly compressible fluid along an optical flow path. The internal volume and the internal geometry of the inlet transition portion configured to minimize turbulence and eddies within the highly compressible fluid as it travels through the optical flow path.

Abstract: The present disclosure relates to methodologies, systems, and devices for extracting CO2 from a mobile phase within a chromatography or extraction system. A mobile phase pump can pump a CO2-based mobile phase through a chromatography column, and a BPR can decompress the mobile phase downstream of the column. Decompressed CO2 can be extracted from the mobile phase using a gas-liquid separator located downstream of the chromatography column. A detector located downstream of the gas-liquid separator can then analyze a substantially liquid mobile phase.

Abstract: Exemplary embodiments are directed to methods and systems for controlling fluid parameters within a detector. A first and second fluid pump manager control the flow of a first and second fluid to one or more heating/cooling devices, and a mixer receives and mixes the first and second fluids. An optical detector flow cell receives the fluid mixture from the mixer, and a pressure regulator controls the pressure at the optical detector flow cell. Thus, the composition, temperature, and pressure of a fluid mixture entering an optical detector flow cell can be controlled in real time.