Patents by Inventor Sebastien DEGOT

Sebastien DEGOT has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11061017
    Abstract: The invention relates to a method for high throughput screening compounds by using a plurality of automated cell based assays assessing skeletal muscle cells contractility, morphology, and metabolism, in order to predict the efficacy of said compound on a panel of applications linked with muscle physiological and pathophysiological processes, comprising: (i) providing an in vitro culture of myotubes, wherein the in vitro myotubes culture is obtained by the following method: providing a cell culture device allowing the culture of myoblasts or myotubes, depositing said cells from a human donor or human group of donors, in good health or affected by a muscle related disorder, from primary cells, a cell line, an isogenic cell line or differentiated stem cells recapitulating a muscle disorder, on said culture device by using a method allowing the spatial control of cell culture, culturing said cells during a determined incubation time so as to promote a spatially controlled myotube culture; (ii) adding at least on
    Type: Grant
    Filed: June 15, 2016
    Date of Patent: July 13, 2021
    Assignee: CYTOO
    Inventors: Sebastien Degot, Yoran Margaron, Amelie Argento-Pucciarelli, Eve Duchemin-Pelletier, Joris Michaud, Mathieu Fernandes, Pauline Poydenot
  • Patent number: 10711248
    Abstract: A method and device for standardizing myoblast differentiation into myotubes, including a substrate (1) and at least one cell-adhesive pattern (2) for culturing myoblasts on the substrate. The pattern (2) has an elongated surface. A central region (2C) and two lateral regions (2L) extend from the central region in both directions along a longitudinal axis of the pattern. The ratio between the maximum width (WC) of the central region (2C) and the maximum width (WL) of the lateral regions (2L) is greater than or equal to 2. The ratio between the length (L) and the maximum width (WC) of the pattern (2) is less than or equal to 4. The method includes providing a device as described above, depositing myoblasts on at least one cell-adhesive pattern of the device, culturing the myoblasts in a differentiation medium to promote cell differentiation into myotubes and constrain elongation of the myotubes.
    Type: Grant
    Filed: December 17, 2014
    Date of Patent: July 14, 2020
    Assignee: CYTOO
    Inventors: Mathieu Fernandes, Sebastien Degot, Yoran Margaron, Jie Liu, Michel Bornens, Maria Luisa Calvo Munoz, Aurelie Berthelot, Alexandra Fuchs, Joanne Young, Delphine Morales
  • Publication number: 20180356400
    Abstract: The invention relates to a method for high throughput screening compounds by using a plurality of automated cell based assays assessing skeletal muscle cells contractility, morphology, and metabolism, in order to predict the efficacy of said compound on a panel of applications linked with muscle physiological and pathophysiological processes, comprising: (i) providing an in vitro culture of myotubes, wherein the in vitro myotubes culture is obtained by the following method: providing a cell culture device allowing the culture of myoblasts or myotubes, depositing said cells from a human donor or human group of donors, in good health or affected by a muscle related disorder, from primary cells, a cell line, an isogenic cell line or differentiated stem cells recapitulating a muscle disorder, on said culture device by using a method allowing the spatial control of cell culture, culturing said cells during a determined incubation time so as to promote a spatially controlled myotube culture; (ii) adding at least on
    Type: Application
    Filed: June 15, 2016
    Publication date: December 13, 2018
    Inventors: SEBASTIEN DEGOT, YORAN MARGARON, AMELIE ARGENTO-PUCCIARELLI, EVE DUCHEMIN-PELLETIER, JORIS MICHAUD, MATHIEU FERNANDES, PAULINE POYDENOT
  • Publication number: 20160312187
    Abstract: The invention relates to a device for standardizing myoblast differentiation into myotubes, comprising a substrate (1) and at least one cell-adhesive pattern (2) for culturing myoblasts on said substrate, wherein: said pattern (2) has an elongated surface comprising a central region (2C) and two lateral regions (2L) extending from said central region in both directions along a longitudinal axis of the pattern with a contour discontinuity between the central region (2C) and each lateral region (2L), the ratio between the maximum width (WC) of the central region (2C) and the maximum width (WL) of the lateral regions (2L) is greater than or equal to 2, the ratio between the length (L) and the maximum width (WC) of the pattern (2) is less than or equal to 4.
    Type: Application
    Filed: December 17, 2014
    Publication date: October 27, 2016
    Inventors: Mathieu FERNANDES, Sebastien DEGOT, Yoran MARGARON, Jie LIU, Michel BORNENS, Maria Luisa CALVO MUNOZ, Aurelie BERTHELOT, Alexandra FUCHS, Joanne YOUNG, Delphine MORALES