Abstract: A method for generating a biological clock including a DNA methylation profile which is suitable for use with at least two different sample types, the method includes: (i) providing a first set of DNA methylation profiles generated from the at least two different sample types from a plurality of subjects; (ii) generating a composite DNA methylation profile from the first set of DNA methylation profiles, wherein the composite DNA methylation profile comprises methylation sites that have a matched status in the different sample types; and (iii) using the composite DNA methylation profile to generate a biological clock using reference DNA methylation profiles from one of the at least two sample types.

Abstract: A method for determining a mortality risk and/or probability of a healthy lifespan of a dog, the method including a) providing a DNA methylation profile from a sample obtained from the dog; and b) determining a mortality risk and/or probability of a healthy lifespan for the dog using the DNA methylation profile. The DNA methylation profile contains at least one methylation site as listed in Table 3.

Abstract: The present disclosure generally relates to mixtures and compositions comprising sulforaphane, or analogue thereof, and at least one amino acid, or analogue thereof. In particular, the disclosure relates the use of the mixtures and compositions for treating or preventing oxidative stress.