Abstract: The present invention relates to a universal calibration method of use for assaying inhibitors of the same enzyme, for example for assaying inhibitors of an enzyme of blood coagulation. The invention also relates to the use of this universal calibration in a method for assaying a reversible or irreversible inhibitor of the enzyme in a biological sample. The invention also relates to the use of the universal calibration in a method for screening inhibitors of the enzyme.

Abstract: Disclosed is an in vitro method for detecting, in a biological sample, the presence of a blood coagulation enzyme inhibitor selected, independently, from factor Xa and factor IIa, the method including the step of making one or more competitive kinetics measurement(s) by carrying out a competitive enzymatic assay and implementing one or more types of classification or regression decision models obtained by training automated supervised learning models. The method may include the identification in the biological sample of an inhibitor in question, or even its characterization, or even its quantification. Also disclosed is a data processing system or device including implementation for at least part of the method, a computer program or storage medium which can be read by a computer or appropriate data, and a suitable kit.

Abstract: The present invention relates to a universal calibration method of use for assaying inhibitors of the same enzyme, for example for assaying inhibitors of an enzyme of blood coagulation. The invention also relates to the use of this universal calibration in a method for assaying a reversible or irreversible inhibitor of the enzyme in a biological sample. The invention also relates to the use of the universal calibration in a method for screening inhibitors of the enzyme.

Abstract: A computer to permit programming by active objects in multi-task mode, representative of systems for simulation, includes a device for real world simulation.