Abstract: The invention relates to a method for counting particles, particularly blood cells, in a sample, using a lensless optical imaging device. The sample is arranged between a light source and an image sensor. The sample is illuminated by a light source and an image is acquired by the image sensor, said image sensor being exposed to a light wave called an exposition wave. A digital propagation operator is applied to the acquired image so as to obtain a complex amplitude of the exposition wave according to a surface facing the image sensor. An image, called a reconstructed image, is formed from the modulus and/or the phase of said complex amplitude, on which image the particles to be counted appear in the form of regions of interest. The method then comprises a step of selecting the regions of interest corresponding to the particles to be counted.

Abstract: The invention relates to a method for counting particles, particularly blood cells, in a sample, using a lensless optical imaging device. The sample is arranged between a light source and an image sensor. The sample is illuminated by a light source and an image is acquired by the image sensor, said image sensor being exposed to a light wave called an exposition wave. A digital propagation operator is applied to the acquired image so as to obtain a complex amplitude of the exposition wave according to a surface facing the image sensor. An image, called a reconstructed image, is formed from the modulus and/or the phase of said complex amplitude, on which image the particles to be counted appear in the form of regions of interest. The method then comprises a step of selecting the regions of interest corresponding to the particles to be counted.

Abstract: The invention relates to a method for detecting, by means of flow cytometry, the presence of normal plasma cells and tumoral plasma cells in a sample of cells from a patient.

Abstract: The invention relates to a method of classifying and flow measuring the refringence of at least two populations of particles present in a fluid. The method uses a light source that has small coherence time, with a coherence length Lc<100 ?m, that is used under extinction conditions at a center wavelength selected as a function of a range of volumes and of a range of refractive indices expected for the particles under consideration. The method uses a device that, together with the light source, forms a converging illuminating beam of aperture angle that is selected as a function of the range of volumes and the range of refractive indices expected for the particles under consideration at the selected center wavelength.

Abstract: The invention relates to a method of classifying and flow measuring the refringence of at least two populations of particles present in a fluid. The method uses a light source that has small coherence time, with a coherence length Lc<100 ?m, that is used under extinction conditions at a center wavelength selected as a function of a range of volumes and of a range of refractive indices expected for the particles under consideration. The method uses a device that, together with the light source, forms a converging illuminating beam of aperture angle that is selected as a function of the range of volumes and the range of refractive indices expected for the particles under consideration at the selected center wavelength.

Abstract: The invention provides a method for use in an automated biological liquid analysis machine that measures at least four physical parameters for each cell detected, the method both performing classification, by discrimination and enumeration, into a set of at least three cell classes and also representing them.