Abstract: The device for the microbiological analysis of a support (41) adapted to contain microorganisms marked by a fluorophore agent comprises illuminating means adapted to illuminate said support (41) comprising two bases (21) on each of which is mounted at least one group of light sources (22), with said sources (22) of that group being regularly spaced from each other to form an array for illuminating said support (41), said bases (21) being fixed to a frame of said device, one on each side of an opening (9) formed in said frame, with said bases (21) being inclined towards each other in the direction of a predetermined location for reception of said support (41) in said device.

Abstract: The preparation unit comprises a body (2) within which are fixed a first (3) and a second (4) membrane, said first membrane (3) having a first predetermined pore diameter and said second membrane (4) a second predetermined pore diameter smaller than said first predetermined pore diameter of said first membrane (3), said body also comprising means (16, 20) for retrieving said sample collected on said second membrane (4). The method of preparing such a sample comprises the step of procuring such a preparation unit (1), the step of passing a predetermined volume of said liquid through said first membrane and through said second membrane (4) in order to collect a sample on said second membrane (4); and the step of retrieving said sample so collected on said second membrane (4).

Abstract: The preparation unit comprises a body (2) within which are fixed a first (3) and a second (4) membrane, said first membrane (3) having a first predetermined pore diameter and said second membrane (4) a second predetermined pore diameter smaller than said first predetermined pore diameter of said first membrane (3), said body also comprising means (16, 20) for retrieving said sample collected on said second membrane (4). The method of preparing such a sample comprises the step of procuring such a preparation unit (1), the step of passing a predetermined volume of said liquid through said first membrane and through said second membrane (4) in order to collect a sample on said second membrane (4); and the step of retrieving said sample so collected on said second membrane (4).

Abstract: The invention concerns a device and a method for concentrating pathogenic germs potentially present in blood products or derivatives and for detecting said germs comprising the following steps: (a) subjecting a sample of said blood product to a blood cell aggregating treatment, (b) eliminating the aggregates formed at step (a) by passing the treated sample over a first filter allowing through the contaminating germs but not the cell aggregates, (c) selectively lyzing the residual cells of the filtrate obtained at step (b), (d) recuperating the contaminating germs by passing the lysate of step (c) over a second filter to detect the contaminating germs possibly trapped.

Abstract: The method of microbiological decontamination of a machine comprising an enclosure (5, 100) for treating a support (6) and a conveyor comprising a shuttle (30), comprises the steps of obtaining a container (6) adapted to be received on said shuttle (30) and to diffuse biocidal agent in said enclosure (5, 100), of placing said container (6) on said shuttle (30), of moving said shuttle (30) within said enclosure, and of evacuating said agent. The microbiological analysis system comprises a container (6), a microbiological analysis machine comprising at least two stations (2, 3, 4) for treatment of said support (6), a conveyor comprising a shuttle (30) adapted to receive and to move said support (6), said container (6) being adapted to be received on said shuttle (30), as well as means (150, 156, 159) for evacuating said biocidal agent.

Abstract: The invention concerns a device for preparing a body fluid for a bacteriological analysis thereof comprising a container provided with a chamber (4) wherein a piston (5) is mobile between an opening position and a closing position, the chamber (4) including a separation zone (7) and means for introducing (15, 16) a fluid into said chamber and the piston (5) including a closure means (8) co-operating with the separation zone (7) so as to define an upper volume (9) and a lower volume (10) on either side of said zone, the upper volume (9) and the lower volume (10) mutually communicating when the piston (5) is in opening position and being tightly isolated from each other when the piston (5) is in closing position. The invention also concerns a preparation method using such a device (1).

Abstract: The invention concerns a device and a method for concentrating pathogenic germs potentially present in blood products or derivatives and for detecting said germs comprising the following steps: (a) subjecting a sample of said blood product to a blood cell aggregating treatment, (b) eliminating the aggregates formed at step (a) by passing the treated sample over a first filter allowing through the contaminating germs but not the cell aggregates, (c) selectively lyzing the residual cells of the filtrate obtained at step (b), (d) recuperating the contaminating germs by passing the lysate of step (c) over a second filter to detect the contaminating germs possibly trapped.

Abstract: The invention concerns a device and a method for concentrating pathogenic germs potentiaily present in blood products or derivatives and for detecting said germs comprising the following steps: (a) subjecting a sample of said blood product to a blood cell aggregating treatment, (b) eliminating the aggregates formed at step (a) by passing the treated sample over a first filter allowing through the contaminating germs but not the cell aggregates, (c) selectively lyzing the residual cells of the filtrate obtained at step (b), (d) recuperating the contaminating germs by passing the lysate of step (c) over a second filter to detect the contaminating germs possibly trapped.

Abstract: A method comprising obtaining a preparation unit comprising a filter module and a collection for each liquid coming from the filter module, with the filter module comprising an inlet compartment as well as an evacuation compartment for the liquids, the filter and collection modules being rotatably mounted relative to each other; disposing the filter and collection modules in a first position; passing a liquid from the filter module to attain the collection module; disposing the filter and collection modules relative to each other in a second position, and passing another liquid from the filter module to attain the collection module.

Abstract: The present invention relates to a method using a composition for permeabilizing microorganism walls for counting and detecting in a targeted manner the microorganisms on a membrane. The invention also relates to a kit and to probes that are suitable for carrying out the method.

Abstract: The unit comprises a filter membrane filter module and a collection module for liquid coming from the filter module, with the filter module comprising an inlet compartment as well as an evacuation compartment for the liquids, the filter and collection modules being rotatably mounted relative to each other. The method comprises the step of obtaining such a unit, of disposing the filter and collection modules in a first position, of passing a liquid from the filter module to attain the collection module, of disposing the filter and collection modules relative to each other in a second position, and the step of passing another liquid from the filter module to attain the collection module.

Abstract: The present invention relates to a composition for the permeabilization of the walls of microorganisms comprising the combination of octyl-?-D-glucopyranoside (NOG), sodium polyphosphates (HMP), and a salt chosen from lithium chloride or rubidium chloride, and a method for the enumeration and identification of cells on a membrane using said composition.

Abstract: The method of microbiological decontamination of a machine comprising an enclosure for treating a support and a conveyor comprising a shuttle, comprises the steps of obtaining a container adapted to be received on the shuttle and to diffuse biocidal agent in the enclosure, of placing the container on the shuttle, of moving the shuttle within the enclosure, and of evacuating the agent. The microbiological analysis system comprises a container, a microbiological analysis machine comprising at least two stations for treatment of the support, a conveyor comprising a shuttle adapted to receive and to move the support, the container being adapted to be received on the shuttle, as well as means for evacuating the biocidal agent.

Abstract: The method of microbiological decontamination of a machine comprising an enclosure (5, 100) for treating a support (6) and a conveyor comprising a shuttle (30), comprises the steps of obtaining a container (6) adapted to be received on said shuttle (30) and to diffuse biocidal agent in said enclosure (5, 100), of placing said container (6) on said shuttle (30), of moving said shuttle (30) within said enclosure, and of evacuating said agent. The microbiological analysis system comprises a container (6), a microbiological analysis machine comprising at least two stations (2, 3, 4) for treatment of said support (6), a conveyor comprising a shuttle (30) adapted to receive and to move said support (6), said container (6) being adapted to be received on said shuttle (30), as well as means (150, 156, 159) for evacuating said biocidal agent.

Abstract: The unit comprises a filter membrane (6) filter module (2) and a collection module (3) for each liquid coming from said filter module (2), with said filter module (2) comprising an inlet compartment as well as an evacuation compartment for the liquids, the filter (2) and collection (3) modules being rotatably mounted relative to each other. The method comprises the step of obtaining such a unit (1), of disposing the filter (2) and collection (3) modules in a first position, of passing a liquid from the filter module (2) to attain the collection module (3), of disposing the filter (2) and collection (3) modules relative to each other in a second position, and the step of passing another liquid from the filter module (2) to attain the collection module (3).

Abstract: A method and device for continuously drawing and mixing liquid samples originating from at least two different containers are described. The device comprises a mixing chamber, which is connected to each of the containers. The device is characterized in that it comprises, between the container and the mixing chamber, at least one intermediate sample chamber for each container, being connected in order to transfer all or a portion of the sampled liquid to the mixing chamber. The device has a vertical configuration.

Abstract: The present invention relates to a composition for permeabilizing microorganism walls, comprising the combination of polyethyleneimine (PEI) with at least one alcohol, and also to a method using said composition for counting and detecting in a targeted manner the microorganisms on a membrane. The invention also relates to a kit and to probes that are suitable for carrying out said method.

Abstract: The device for the microbiological analysis of a support (41) adapted to contain microorganisms marked by a fluorophore agent comprises illuminating means adapted to illuminate said support (41) comprising two bases (21) on each of which is mounted at least one group of light sources (22), with said sources (22) of that group being regularly spaced from each other to form an array for illuminating said support (41), said bases (21) being fixed to a frame of said device, one on each side of an opening (9) formed in said frame, with said bases (21) being inclined towards each other in the direction of a predetermined location for reception of said support (41) in said device.

Abstract: The unit comprises a filter membrane (6) filter module (2) and a collection module (3) for each liquid coming from said filter module (2), with said filter module (2) comprising an inlet compartment as well as an evacuation compartment for the liquids, the filter (2) and collection (3) modules being rotatably mounted relative to each other. The method comprises the step of obtaining such a unit (1), of disposing the filter (2) and collection (3) modules in a first position, of passing a liquid from the filter module (2) to attain the collection module (3), of disposing the filter (2) and collection (3) modules relative to each other in a second position, and the step of passing another liquid from the filter module (2) to attain the collection module (3).

Abstract: The present invention relates to a composition for the permeabilization of the walls of microorganisms comprising the combination of octyl-?-D-glucopyranoside (NOG), sodium polyphosphates (HMP), and a salt chosen from lithium chloride or rubidium chloride, and a method for the enumeration and identification of cells on a membrane using said composition.