Abstract: A method and composition for use therein for measuring the activity of creatine kinase (hereinafter referred to as "CK") having good storage stability is disclosed. More particularly, a composition for measurement of CK activity which comprises as main components phosphoglycerate kinase (hereinafter referred to a "PGK") which catalyzes the following reaction 2 and glyceraldehyde-3-phosphate dehydrogenase (hereinafter referred to as "GAPDH") which catalyzes the following reaction 3. Reaction 1 is catalyzed by the creatine kinase.Reaction 1Creatinephosphate+Adenosine-5'-diphosphate (hereinafter referred to as "ADP").revreaction.Creatine+Adenosine-5'-triphosphate (hereinafter referred to as "ATP")Reaction 2ATP+3-Phosphoglycerate.revreaction.ADP+1,3-DiphosphoglycerateReaction 31,3-Diphosphoglycerate+Reduce .beta.-nicotinamideadenine dinucleotide (hereinafter referred to as "NADH").revreaction.Glyceraldehyde-3-phosphate+.beta.

Abstract: This invention relates to a process for producing collagenase, which comprises aerobically cultivating a collagenase-producing bacterium belonging to the genus Bacillus, and recovering collagenase from the culture broth.This invention also provides a thermophilic bacterium of the genus Bacillus which has the ability to produce collagenase and which grows at a temperature of 42.degree. to 74.degree. C. and a pH of 5.0 to 9.0 and a novel thermophilic collagenase which does not substantially lose collagenase activity even when maintained for 1 hour at a temperature of 60.degree. C. in the presence of a calcium ion at a pH in the range of 6.5 to 8.5, and which has a molecular weight, determined by gel filtration, between those of cytochrome C and ovalbumin.