Abstract: A method for introducing a genome editing enzyme into a plant cell includes: treating the cell with plasma; and then bringing the cell into contact with the genome editing enzyme in the presence of a di- or higher-valent metal cation.

Abstract: The present inventors found that the efficiency of introducing a protein into a plant dramatically increases as compared with conventional methods for cultivating a bacteria-infected plant as it is when the protein of interest is expressed with bacteria having the type III secretion system, the bacteria are brought into contact with the plant, and then the infected tissues are cultured under bacteriostatic conditions for a certain period of time.

Abstract: The present inventors found that the efficiency of introducing a protein into a plant dramatically increases as compared with conventional methods for cultivating a bacteria-infected plant as it is when the protein of interest is expressed with bacteria having the type III secretion system, the bacteria are brought into contact with the plant, and then the infected tissues are cultured under bacteriostatic conditions for a certain period of time.

Abstract: A combination of virus vectors for genome editing is formed by arranging a polynucleotide encoding a split genome editing enzyme in each of a Tobamovirus vector and a Potexvirus vector and arranging a polynucleotide encoding a guide RNA in one of the vectors. It is found that when these virus vectors are introduced into a plant cell, a complex of a functional Cas9 protein and the guide RNA is formed in the plant cell, and a genome is edited in a target site-specific manner.

Abstract: A method for producing a plant cell comprising a mutation introduced in a target DNA comprises: a step of introducing into plant cells a DNA construct comprising a DNA homologous to a target DNA, wherein a desired mutation is introduced and a piggyBac transposon containing a marker gene is inserted in the homologous DNA; a step of selecting a plant cell, in which the mutation and the piggyBac transposon are introduced in the target DNA via homologous recombination, based on an expression of the marker gene; and a step of removing the piggyBac transposon from the target DNA by constitutively expressing a piggyBac transposase in the cell selected in the above step.

Abstract: It has been found that a marker gene, which is inserted in the genomic DNA via homologous recombination, and to both ends of which nuclease recognition sites are added, can be removed from a plant cell by using a corresponding nuclease, and further that the nuclease recognition sites can also be removed without leaving any trace by matching sequences of at least 30 nucleotides adjacent to the recognition sites. Moreover, in a method for introducing a mutation into a target DNA on the genome of a plant cell via homologous recombination, it is made possible to: stably select a plant cell, in which the mutation is introduced, based on an expression of a marker gene; further, to remove an unnecessary sequence such as the marker gene from the selected cell; and to introduce only a required mutation into the target DNA.

Abstract: A method for producing a plant cell comprising a mutation introduced in a target DNA comprises: a step of introducing into plant cells a DNA construct comprising a DNA homologous to a target DNA, wherein a desired mutation is introduced and a piggyBac transposon containing a marker gene is inserted in the homologous DNA; a step of selecting a plant cell, in which the mutation and the piggyBac transposon are introduced in the target DNA via homologous recombination, based on an expression of the marker gene; and a step of removing the piggyBac transposon from the target DNA by constitutively expressing a piggyBac transposase in the cell selected in the above step.

Abstract: It has been found that a marker gene, which is inserted in the genomic DNA via homologous recombination, and to both ends of which nuclease recognition sites are added, can be removed from a plant cell by using a corresponding nuclease, and further that the nuclease recognition sites can also be removed without leaving any trace by matching sequences of at least 30 nucleotides adjacent to the recognition sites. Moreover, in a method for introducing a mutation into a target DNA on the genome of a plant cell via homologous recombination, it is made possible to: stably select a plant cell, in which the mutation is introduced, based on an expression of a marker gene; further, to remove an unnecessary sequence such as the marker gene from the selected cell; and to introduce only a required mutation into the target DNA.

Abstract: It has been found that the use of a plant cell in which a function of a protein involved in repair by nonhomologous end joining is artificially suppressed dramatically increases the efficiency of introductions of non-silent mutations in a repairing process by nonhomologous end joining which occurs after induction of a DNA double-strand break with a zinc finger nuclease.

Abstract: Provided is a method of transforming a monocotyledon by using agrobacterium. There is provided a method of transforming a monocotyledon by using agrobacterium containing a desired recombinant gene. This transforming method comprises the steps of sowing a culture medium containing a plant growth factor with a monocotyledonous seed, conducting culturing for 1 to 3 days so as to effect germination and infecting the seed with agrobacterium. This method makes it feasible to rapidly transform monocotyledons, including a rice plant.

Abstract: A method of transforming a monocotyledon by means of agrobacterium. There is provided a method of transforming a monocotyledon by means of agrobacterium containing a desired recombinant gene. This transforming method comprises the steps of sowing a culture medium containing a plant growth factor with a monocotyledonous seed, conducting culturing for 1 to 3 days so as to effect germination and infecting the seed with agrobacterium. This method makes it feasible to rapidly transform monocotyledons, including a rice plant.

Abstract: The present invention provides methods for inducing tissue-specific expression, which includes preparing an expression vector having a promoter. The promoter includes any one of the following DNAs (a) to (c): (a) a DNA, which includes the nucleotide sequence shown in SEQ ID NO: 1; (b) a DNA, which includes the nucleotide sequence ranging from nucleotides 1344 to 2843 of SEQ ID NO: 1; and (c) a DNA, which is a DNA fragment that can hybridize under stringent conditions to a DNA fragment including a nucleotide sequence fully complementary to the nucleotide sequence shown in SEQ ID NO: 1, wherein (a), (b) or (c) can control the transcription of a coding sequence located downstream thereof; and wherein the stringent conditions are 5×SSC at 42° C.; The expression vector, also, includes a coding sequence located downstream of the promoter.

Abstract: The present inventors conceived that a marker gene may be efficiently and easily removed by using the floral dip method to transiently express high levels of a site-specific recombinase gene. it is considered that the present methods can also be applied to methods for transposing a transposon that does not have a transposase from a transformed plant that comprises the transposon having no transposase.

Abstract: The present invention enables characteristic gene expression control and relates to a promoter, which comprises any one of the following DNAs (a) to (c): (a) a DNA, which comprises the nucleotide sequence shown in SEQ ID NO: 1; (b) a DNA, which comprises a nucleotide sequence derived from the nucleotide sequence shown in SEQ ID NO: 1 by deletion, substitution, insertion, or addition of 1 or a plurality of nucleotides, and which can control transcription of a gene located downstream thereof; and (c) a DNA, which is a DNA fragment that can hybridize under stringent conditions to a DNA fragment comprising a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 1, and which can control the transcription of a gene located downstream thereof.

Abstract: A C3 plant has a gene for an enzyme involved in a C4 pathway of photosynthesis and expresses the C4 photosynthesis gene at a high level. More specifically, the C3 plant includes DNA which contains (a) an expression control region of a gene for an enzyme involved in a photosynthetic pathway of a phylogenetically related C4 plant and (b) a structural gene for an enzyme involved in a photosynthetic pathway of the C4 plant. The C3 plant expresses the enzyme encoded by the structural gene at a high level.

Abstract: A C3 plant has a gene for an enzyme involved in a C4 pathway of photosynthesis and expresses the C4 photosynthesis gene at a high level. More specifically, the C3 plant includes DNA which contains (a) an expression control region of a gene for an enzyme involved in a photosynthetic pathway of a phylogenetically related C4 plant and (b) a structural gene for an enzyme involved in a photosynthetic pathway of the C4 plant. The C3 plant expresses the enzyme encoded by the structural gene at a high level.