Patents by Inventor Seiko Onai

Seiko Onai has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Process for producing peptides in E. coli
    Patent number: 5670340
    Abstract: The present invention is a process to express a target peptide in a large amount and accumulate the target peptide in host cells in the form of inclusion bodies. The process comprises:A) culturing host cells transformed with a plasmid able to express a gene coding for a fusion protein represented in the formula A--L--B, wherein B is a target peptide, A is a protective peptide comprising a 90-210 amino acid fragment E. coli .beta.-galactosidase, and L is a linker peptide positioned between the C-terminus of the protective peptide and the N-terminus of the target peptide and selected so that when the fusion protein is treated by an enzyme or chemical substance, the target peptide is separated, and wherein the protective peptide and linker peptide are selected so that the isoelectric point of the fusion protein in between 4.9 and 6.
    Type: Grant
    Filed: December 5, 1994
    Date of Patent: September 23, 1997
    Assignee: Suntory Limited
    Inventors: Masayuki Yabuta, Yuji Suzuki, Kazuhiro Ohsuye, Takehiro Oshima, Seiko Onai, Koji Magota, Shoji Tanaka