Abstract: Disclosed and claimed are isolated nucleic acid molecules encoding Bacillus stearothermophilus DNA polymerase (DNApolI), including the structural gene for DNApolI, such as DNApolI genes having insertions, deletions, inactivation, or mutations at the 5' end thereof and thus encode Bst polymerase I enzymes which lack or have reduced 3'-5' exonuclease activity, as well as methods for making and using such nucleic acid molecules and such polymerases. For instance, the nucleic acid molecules are useful for making the polymerases, for example, by expression of a vector comprising the nucleic acid molecules; and, the polymerases are useful in DNA sequencing and/or labelling. Thus, disclosed and claimed recombinant DNA clones corresponding to the Bacillus stearothermophilus DNA polymerase (DNApolI) structural gene with deletions at the 5' end. The polymerases from these recombinant DNA clones exhibit DNA synthesis domain activity but have reduced 3' to 5' exonuclease activity.