Abstract: The invention provides differential refractive index detectors and methods for the use of differential refractive index detectors. In an exemplary embodiment, a differential refractive index detector includes a flow cell body having a proximal end, a distal end, and a flow axis extending between the proximal and the distal end. The flow cell body includes a first chamber and a second chamber and the fluid conduits coupled to the flow cell body can be tapered to reduce dispersion.

Abstract: The present technology relates to methodologies, systems and apparatus for separating a liquid and a gas from a multi-phase flow stream. In particular, a gas-liquid separator having a curvilinear flow path sized is described. The flow path is designed to create a shift in the axial velocity of the primary flow field through the gas-liquid separator and generate a secondary flow field effect perpendicular to the primary flow field. The curvilinear flow path can minimize additional dispersion and provide improved efficiencies in fraction collection.

Abstract: The present disclosure relates to methodologies, systems and apparatus for cooling a supersaturated or mixed-phase solution to increase the solubility of gases dissolved therein. In a fluid chromatography system, the mobile phase may include a pressurized mixture including a gas, such as CO2, and prior to fraction collection and/or detection a portion of the gas is extracted via a gas/liquid separator. Cooling the substantially liquid phase eluent exiting the gas/liquid separator increases the solubility of any remnant gas still dissolved within the eluent. The increased solubility of the remnant gas prevents out gassing, provides more consistent flow of the eluent through the system, and results in more efficient collection and analysis of a desired sample.

Abstract: Methods, systems and apparatus are provided for degassing a supersaturated solution. An example degasser is described having a continuous body of gas-permeable tubing to remove an amount of a gas from the supersaturated solution below the gas's saturation point in the supersaturated solution. The degasser can be connected to at least one of a fraction collector or a detector. The example degasser is sized and/or positioned to cause a change in pressure (?P) across the degasser to drive removal of a dissolved gas from a supersaturated solution passing through the degasser. As a result of the reduction of gas, efficiencies in system flow and fraction collection are achieved.

Abstract: The invention provides differential refractive index detectors and methods for the use of differential refractive index detectors. In an exemplary embodiment, a differential refractive index detector includes a flow cell body having a proximal end, a distal end, and a flow axis extending between the proximal and the distal end. The flow cell body includes a first chamber and a second chamber and the fluid conduits coupled to the flow cell body can be tapered to reduce dispersion.

Abstract: The invention provides differential refractive index detectors and methods for the use of differential refractive index detectors. In an exemplary embodiment, a differential refractive index detector includes a flow cell body having a proximal end, a distal end, and a flow axis extending between the proximal and the distal end. The flow cell body includes a first chamber and a second chamber and the fluid conduits coupled to the flow cell body can be tapered to reduce dispersion.

Abstract: The present disclosure relates to methodologies, systems and apparatus for cooling a supersaturated or mixed-phase solution to increase the solubility of gases dissolved therein. In a fluid chromatography system, the mobile phase may include a pressurized mixture including a gas, such as CO2, and prior to fraction collection and/or detection a portion of the gas is extracted via a gas/liquid separator. Cooling the substantially liquid phase eluent exiting the gas/liquid separator increases the solubility of any remnant gas still dissolved within the eluent. The increased solubility of the remnant gas prevents out gassing, provides more consistent flow of the eluent through the system, and results in more efficient collection and analysis of a desired sample.

Abstract: Methods, systems and apparatus are provided for degassing a supersaturated solution. An example degasser is described having a continuous body of gas-permeable tubing to remove an amount of a gas from the supersaturated solution below the gas's saturation point in the supersaturated solution. The degasser can be connected to at least one of a fraction collector or a detector. The example degasser is sized and/or positioned to cause a change in pressure (?P) across the degasser to drive removal of a dissolved gas from a supersaturated solution passing through the degasser. As a result of the reduction of gas, efficiencies in system flow and fraction collection are achieved.

Abstract: The present technology relates to methodologies, systems and apparatus for separating a liquid and a gas from a multi-phase flow stream. In particular, a gas-liquid separator having a curvilinear flow path sized is described. The flow path is designed to create a shift in the axial velocity of the primary flow field through the gas-liquid separator and generate a secondary flow field effect perpendicular to the primary flow field. The curvilinear flow path can minimize additional dispersion and provide improved efficiencies in fraction collection.

Abstract: The invention provides differential refractive index detectors and methods for the use of differential refractive index detectors. In an exemplary embodiment, a differential refractive index detector includes a flow cell body having a proximal end, a distal end, and a flow axis extending between the proximal and the distal end. The flow cell body includes a first chamber and a second chamber and the fluid conduits coupled to the flow cell body can be tapered to reduce dispersion.