Abstract: The present disclosure provides a method of producing an immune cell culture utilizing a fully closed system.

Abstract: The present invention provides methods for producing allogeneic T-cells, including the use of an engineered nuclease under the control of a controllable promoter. By preparing T-cells with an inducible nuclease, large volumes of cells can be prepared, each of which contains the ability to individually produce the desired nuclease. These cells can then be modified as desired through the introduction of a gene of interest, or an undesired gene can be knocked-out. Also provided herein are allogeneic T-cells for use in various therapeutic applications.

Abstract: A system and method for handling one or more dependency services hosted by one or more dependency servers for an upstream service hosted by an administrative server in a distributed computer architecture is provided. The present invention provides for identifying any abnormality in the behavior of the dependency services on the basis of metric values associated with service-parameters of said dependency services. Further, the resiliency services are enabled in order to handle one or more faltering dependency services based on the faulty metric values associated with the service-parameters. Yet further, the one or more faltering dependency services are continuously monitored, and one or more resiliency services are withdrawn once the fault in said dependency services is resolved. Yet further, the present invention provides a conversational bot interface for managing the administrative server and associated dependency services.

Abstract: A system and method for handling one or more dependency services hosted by one or more dependency servers for an upstream service hosted by an administrative server in a distributed computer architecture is provided. The present invention provides for identifying any abnormality in the behavior of the dependency services on the basis of metric values associated with service-parameters of said dependency services. Further, the resiliency services are enabled in order to handle one or more faltering dependency services based on the faulty metric values associated with the service-parameters. Yet further, the one or more faltering dependency services are continuously monitored, and one or more resiliency services are withdrawn once the fault in said dependency services is resolved. Yet further, the present invention provides a conversational bot interface for managing the administrative server and associated dependency services.

Abstract: The invention relates generally to solid supports for chromatography. In specific embodiments the invention provides for solid supports suitable for affinity chromatography along with methods, systems and kits which use the same.

Abstract: The invention relates generally to solid supports for chromatography. In specific embodiments the invention provides for solid supports suitable for affinity chromatography along with methods, systems and kits which use the same.

Abstract: The invention relates generally to solid supports for chromatography. In specific embodiments the invention provides for solid supports suitable for affinity chromatography along with methods, systems and kits which use the same.

Abstract: Media and devices, such as nitrocellulose-based filters, wherein the media is coated with a polymer such as a polyallylamine. The resulting device offers strong binding of protein impurities and superior removal of host cell proteins from biological samples.

Abstract: Media and devices, such as depth filters including such media, wherein the media is impregnated with a polymer such as a polyallylamine. The resulting device offers strong binding of protein impurities and superior removal of host cell proteins from biological samples.

Abstract: A method of purifying biomolecules with an anion exchanger containing a membrane having a surface having a polymer such as a primary or secondary amine ligand formed thereon, such as polyallylamine. The feedstock is introduced to the exchanger in the presence of one or more ionic-modifiers by themselves or in combination with monovalent salt. The ionic modifier alters the binding ability of the primary amines such that they retain a significant binding capacity for highly charged species such as DNA but lose part or almost all of their binding capacity for less charged species such as viruses or proteins at pH above the pI of the virus or protein.

Abstract: Media and devices, such as nitrocellulose-based filters, wherein the media is coated with a polymer such as a polyallylamine. The resulting device offers strong binding of protein impurities and superior removal of host cell proteins from biological samples.

Abstract: Media and devices, such as depth filters including such media, wherein the media is impregnated with a polymer such as a polyallylamine. The resulting device offers strong binding of protein impurities and superior removal of host cell proteins from biological samples.

Abstract: The present invention relates, at least in part, to novel and improved flow-through purification processes for separating large biomolecules, such as, for example, encapsulated viruses, virus-like particles and conjugate vaccines from one or more contaminants in a sample, where the process employs the use of at least one population of a solid porous particle which comprises a minimized external surface area per unit volume of the particles and an internal surface area per unit volume which is not decreased by more than 25% relative to a population of a similar particle which does not have a minimized external surface area.