Patents by Inventor Senthil Ramu
Senthil Ramu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11845054Abstract: Methods and apparatus relate to the synthesis of polynucleotides having a predefined sequence on a support. Assembly methods include primer extension to generate overlapping construction oligonucleotides and assembly of the polynucleotides of interest onto an anchor support-bound oligonucleotides. Methods and apparatus for selection of polynucleotides having the predefined sequence and/or length are disclosed.Type: GrantFiled: July 16, 2021Date of Patent: December 19, 2023Assignee: Gen9, Inc.Inventors: Joseph Jacobson, Li-Yun A. Kung, Andrew Kirk Wilson, Senthil Ramu, Daniel Schindler, Michael E. Hudson
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Publication number: 20230142867Abstract: The invention described herein provides methods and compositions for using recombinant AAV-based viral vectors to express any gene-of-interest (GOI) under its native promoter and a proprietary heterologous enhancer, preferably expressing a GOI defective in a neuronal disease or disorder in order to treat the neuronal disease or disorder.Type: ApplicationFiled: April 23, 2021Publication date: May 11, 2023Inventor: Senthil Ramu
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Publication number: 20220298514Abstract: The invention described herein provides methods and compositions for using recombinant AAV-based viral vectors to express any gene-of-interest (GOI), preferably from muscle and/or liver tissues, for secretion of the polypeptides encoded by the GOI into the bloodstream, thus serving as an alternative treatment regimen for diseases caused by or characterized by lack of functional GOI, or for disease caused by or characterized by a pathological antigen that can be neutralized by a neutralizing peptide encoded by the GOI, such as an immunoglobulin, antibody, or antigen-binding fragment thereof. The method/composition of the invention can be used as an alternative for enzyme replacement therapy (ERT), or for antibody-based therapy.Type: ApplicationFiled: September 10, 2020Publication date: September 22, 2022Inventor: Senthil Ramu
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Publication number: 20220031865Abstract: The invention described herein provides gene therapy vectors, such as adeno-associated virus (AAV) vectors, that co-express a functional protein (such as a miniaturized human micro-dystrophin gene product) and one or more additional coding sequences for an RNAi sequence (siRNA, shRNA, miRNA), an antisense sequence, a guide sequence for a gene editing enzyme (such as an sgRNA for CRISPR/Cas9, or a crRNA for CRISPR/Casl2a), and/or a micro RNA, and methods of using the vectors to treat subjects suffering from a muscular dystrophy such as DMD/BMD.Type: ApplicationFiled: December 11, 2019Publication date: February 3, 2022Inventors: Senthil Ramu, Joel Schneider, Kathy Ye Morgan, Wen Allen Tseng, Fatih Ozsolak, Meghan Soustek-Kramer, Eric Reyes, Sarath Mandava
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Publication number: 20210339219Abstract: Methods and apparatus relate to the synthesis of polynucleotides having a predefined sequence on a support. Assembly methods include primer extension to generate overlapping construction oligonucleotides and assembly of the polynucleotides of interest onto an anchor support-bound oligonucleotides. Methods and apparatus for selection of polynucleotides having the predefined sequence and/or length are disclosed.Type: ApplicationFiled: July 16, 2021Publication date: November 4, 2021Applicant: Gen9, Inc.Inventors: Joseph Jacobson, Li-Yun A. Kung, Andrew Kirk Wilson, Senthil Ramu, Daniel Schindler, Michael E. Hudson
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Patent number: 11084014Abstract: Methods and apparatus relate to the synthesis of polynucleotides having a predefined sequence on a support. Assembly methods include primer extension to generate overlapping construction oligonucleotides and assembly of the polynucleotides of interest onto an anchor support-bound oligonucleotides. Methods and apparatus for selection of polynucleotides having the predefined sequence and/or length are disclosed.Type: GrantFiled: August 7, 2019Date of Patent: August 10, 2021Assignee: Gen9, Inc.Inventors: Joseph Jacobson, Li-yun A. Kung, Andrew Kirk Wilson, Senthil Ramu, Daniel Schindler, Michael Hudson
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Publication number: 20210009991Abstract: Methods and apparatus relate to reduction of sequence errors generated during synthesis of nucleic acids on a microarray chip. The error reduction can include synthesis of complementary stands (to template strands), using a short universal primer complementary to the template strands and polymerase. Heteroduplex can be formed be melting and re-annealing complementary stands and template strands. The heteroduplexes containing a mismatch can be recognized and cleaved by a mismatch endonuclease. The mismatch-containing cleaved heteroduplexes can be removed from the microarray chip using a global buffer exchange. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products.Type: ApplicationFiled: September 25, 2020Publication date: January 14, 2021Applicant: Gen9, Inc.Inventors: Senthil Ramu, Joseph Jacobson
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Patent number: 10829759Abstract: Methods and apparatus relate to reduction of sequence errors generated during synthesis of nucleic acids on a microarray chip. The error reduction can include synthesis of complementary stands (to template strands), using a short universal primer complementary to the template strands and polymerase. Heteroduplex can be formed be melting and re-annealing complementary stands and template strands. The heteroduplexes containing a mismatch can be recognized and cleaved by a mismatch endonuclease. The mismatch-containing cleaved heteroduplexes can be removed from the microarray chip using a global buffer exchange. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products.Type: GrantFiled: July 19, 2016Date of Patent: November 10, 2020Assignee: Gen9, Inc.Inventors: Senthil Ramu, Joseph Jacobson
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Publication number: 20200197896Abstract: Methods and apparatus relate to the synthesis of polynucleotides having a predefined sequence on a support. Assembly methods include primer extension to generate overlapping construction oligonucleotides and assembly of the polynucleotides of interest onto an anchor support-bound oligonucleotides. Methods and apparatus for selection of polynucleotides having the predefined sequence and/or length are disclosed.Type: ApplicationFiled: August 7, 2019Publication date: June 25, 2020Applicant: Gen9, Inc.Inventors: Joseph Jacobson, Li-yun A. Kung, Andrew Kirk Wilson, Senthil Ramu, Daniel Schindler, Michael Hudson
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Publication number: 20190143291Abstract: Methods and devices are provided for manipulating droplets on a support using surface tension properties, moving the droplets along a predetermined path and merging two droplets together enabling a number of chemical reactions. Disclosed are methods for controlling the droplets volumes. Disclosed are methods and devices for synthesizing at least one oligonucleotide having a predefined sequence. Disclosed are methods and devices for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides having a predefined sequence.Type: ApplicationFiled: December 21, 2018Publication date: May 16, 2019Inventors: Joseph Jacobson, Larry Li-Yang Chu, Senthil Ramu
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Patent number: 10207240Abstract: Methods and devices are provided for manipulating droplets on a support using surface tension properties, moving the droplets along a predetermined path and merging two droplets together enabling a number of chemical reactions. Disclosed are methods for controlling the droplets volumes. Disclosed are methods and devices for synthesizing at least one oligonucleotide having a predefined sequence. Disclosed are methods and devices for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides having a predefined sequence.Type: GrantFiled: November 3, 2010Date of Patent: February 19, 2019Assignee: Gen9, Inc.Inventors: Joseph Jacobson, Larry Li-Yang Chu, Senthil Ramu
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Publication number: 20160326520Abstract: Methods and apparatus relate to reduction of sequence errors generated during synthesis of nucleic acids on a microarray chip. The error reduction can include synthesis of complementary stands (to template strands), using a short universal primer complementary to the template strands and polymerase. Heteroduplex can be formed be melting and re-annealing complementary stands and template strands. The heteroduplexes containing a mismatch can be recognized and cleaved by a mismatch endonuclease. The mismatch-containing cleaved heteroduplexes can be removed from the microarray chip using a global buffer exchange. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products.Type: ApplicationFiled: July 19, 2016Publication date: November 10, 2016Inventors: Senthil Ramu, Joseph Jacobson
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Publication number: 20160272934Abstract: Microfluidic devices for dissociating tissue, culturing, separating, manipulating, and assaying cells and methods for manufacturing and using the devices are disclosed. Individual modules for tissue dissociation, cell, protein and particle separation, cell adhesion to functionalized, permissive micro- and nano-substrates, cell culturing, cell manipulation, cell and extracellular component assaying via metabolic and therapeutic compounds are described. Specialized micro- and nano-substrates and their methods of fabrication are also described. An integrated device is also disclosed. The devices and methods can be used for diagnostic applications, monitoring of disease progression, analysis of disease recurrence, compound discovery, compound validation, drug efficacy screening, and cell-based assays.Type: ApplicationFiled: October 22, 2014Publication date: September 22, 2016Applicant: CELLANYX DIAGNOSTICS, LLCInventors: Ashok C. CHANDER, Wendell R. SU, Thiagarajan MEYYAPPAN, Jonathan VARSANIK, Senthil RAMU, Michael MANAK, Brad HOGAN
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Publication number: 20160250613Abstract: Methods and apparatus relate to the synthesis of polynucleotides having a predefined sequence on a support. Assembly methods include primer extension to generate overlapping construction oligonucleotides and assembly of the polynucleotides of interest onto an anchor support-bound oligonucleotides. Methods and apparatus for selection of polynucleotides having the predefined sequence and/or length are disclosed.Type: ApplicationFiled: February 25, 2016Publication date: September 1, 2016Inventors: Joseph Jacobson, Li-yun A. Kung, Andrew Kirk Wilson, Senthil Ramu, Daniel Schindler, Michael Hudson
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Patent number: 9422600Abstract: Methods and apparatus relate to reduction of sequence errors generated during synthesis of nucleic acids on a microarray chip. The error reduction can include synthesis of complementary stands (to template strands), using a short universal primer complementary to the template strands and polymerase. Heteroduplex can be formed be melting and re-annealing complementary stands and template strands. The heteroduplexes containing a mismatch can be recognized and cleaved by a mismatch endonuclease. The mismatch-containing cleaved heteroduplexes can be removed from the microarray chip using a global buffer exchange. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products.Type: GrantFiled: June 20, 2011Date of Patent: August 23, 2016Assignee: Gen9, Inc.Inventors: Senthil Ramu, Joseph Jacobson
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Publication number: 20160097051Abstract: Disclosed are compositions, methods and devices for the in situ synthesis of nucleic acids. In an exemplary embodiment, a support-bound oligonucleotide is elongated by addition of one or more nucleotides by hybridization of a partially double-stranded oligonucleotide, ligation and removal of unwanted nucleotides.Type: ApplicationFiled: October 16, 2015Publication date: April 7, 2016Inventors: Joseph Jacobson, Senthil Ramu, Daniel Schindler
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Patent number: 9295965Abstract: Methods and apparatus relate to the synthesis of polynucleotides having a predefined sequence on a support. Assembly methods include primer extension to generate overlapping construction oligonucleotides and assembly of the polynucleotides of interest onto an anchor support-bound oligonucleotides. Methods and apparatus for selection of polynucleotides having the predefined sequence and/or length are disclosed.Type: GrantFiled: November 10, 2011Date of Patent: March 29, 2016Assignee: Gen9, Inc.Inventors: Joseph Jacobson, Li-yun A. Kung, Andrew Kirk Wilson, Senthil Ramu, Daniel Schindler, Mike Hudson
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Patent number: 9187777Abstract: Disclosed are compositions, methods and devices for the in situ synthesis of nucleic acids. In an exemplary embodiment, a support-bound oligonucleotide is elongated by addition of one or more nucleotides by hybridization of a partially double-stranded oligonucleotide, ligation and removal of unwanted nucleotides.Type: GrantFiled: May 26, 2011Date of Patent: November 17, 2015Assignee: Gen9, Inc.Inventors: Joseph Jacobson, Senthil Ramu, Daniel Schindler
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Publication number: 20130309725Abstract: Disclosed are compositions, methods and devices for the in situ synthesis of nucleic acids. In an exemplary embodiment, a support-bound oligonucleotide is elongated by addition of one or more nucleotides by hybridization of a partially double-stranded oligonucleotide, ligation and removal of unwanted nucleotides.Type: ApplicationFiled: May 26, 2011Publication date: November 21, 2013Applicant: GEN9, INC.Inventors: Joseph Jacobson, Senthil Ramu, Daniel Schindler
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Publication number: 20130296194Abstract: Methods and apparatus relate to the synthesis of polynucleotides having a predefined sequence on a support. Assembly methods include primer extension to generate overlapping construction oligonucleotides and assembly of the polynucleotides of interest onto an anchor support-bound oligonucleotides. Methods and apparatus for selection of polynucleotides having the predefined sequence and/or length are disclosed.Type: ApplicationFiled: November 10, 2011Publication date: November 7, 2013Applicant: Gen9, Inc.Inventors: Joseph Jacobson, Li-yun A. Kung, Andrew Kirk Wilson, Senthil Ramu, Daniel Schindler, Mike Hudson