Abstract: Provided is a cup holding assembly and a dental cup dispenser using same, the cup holding assembly including a plurality of holding modules, each has a seat portion and can hold a cup by contacting the cup from a plurality of directions in a mounting space and a rotary plate which has an inner opening overlapping with the mounting space and includes at least one spiral rail coupled to each of the plurality of seat portions in a non-fixed manner, thereby providing a spiral guide path to each of the plurality of seat portions. Each of the plurality of seat portions moves along the spiral guide path according to the rotation of the rotary plate. The plurality of holding modules move in a direction toward or away from the mounting space or the center of the first inner opening according to the movements of the plurality of seat portions.