Abstract: The present invention relates to an intracellular expression enhancing genetic element, which enhances the intracellular expression of target protein encoded in RNA therapeutics such as mRNA vaccines and RNA medicines. In more detail, the present invention relates to the RNA molecule which has the genetic element capable of enhancing the intracellular expression of target protein encoded in RNA therapeutics delivered into cells; the plasmid used to synthesize the RNA molecule having the genetic element “E3”; the method for synthesizing RNA molecule having the genetic element “E3” using the plasmid and utilizing the synthesized RNA molecule as RNA therapeutics.

Abstract: An appliance, system, and method for operating an appliance and determining presence of water softener at water provided to an appliance is provided. The system is configured to obtain a first water hardness value; obtain a second water hardness value based on a location of the appliance; determine a ratio of the first water hardness value and the second water hardness value; compare the ratio of the first water hardness value and the second water hardness value to a threshold limit; transmit a first water hardness signal when the threshold limit is exceeded, wherein the first water hardness signal corresponds to the first water hardness value; transmit, to the database storage device, a second water hardness signal when the ratio is within the threshold limit; and adjust operation of the appliance based on whether the first water hardness signal or the second water hardness signal is transmitted.

Abstract: An appliance, system, and method for operating an appliance is provided. The appliance, system, and method include performing a wash cycle and draining wash water from the wash chamber, flowing a quantity of water to a wash chamber, determining a water hardness value based on a water conductivity measurement and a temperature measurement, and draining the quantity of water from the wash chamber.

Abstract: A dryer appliance and a method of operating a dryer appliance are provided. The dryer includes a drum defining a drying chamber rotatably mounted within a cabinet, an additive dispensing assembly to provide a dryer additive to the drying chamber, and a controller for initiating a drying operation. The controller obtains washer operating parameters from a washing machine, determines a drying operation based on the received parameters, initiates a dry cycle, and directs a dryer additive to be dispensed based on the dryer operating schedule.

Abstract: A method of operating a washing machine appliance includes measuring a humidity value with the humidity sensor and comparing the measured humidity value to an expected humidity value. The method also includes flagging a fault in response to the measured humidity value differing from the expected humidity value by at least a predetermined threshold.

Abstract: An apparatus and a method for measuring the RPM of a fan by using an IR-UWB radar are disclosed. The disclosed apparatus comprises: a radar signal variance acquisition unit which receives a reflected signal of an IR-UWB radar to acquire the variance of a signal at each location; a fan location acquisition unit for acquiring a first location and a second location, associated with the location of a fan, by using the variance of the signal at each location; an FFT calculation unit which performs FFT calculation for the signal of the first location to acquire a first FFT signal and which performs FFT calculation for the signal of the second location to acquire a second FFT signal; a subtraction unit for subtracting the second FFT signal from the first FFT signal; and an RPM acquisition unit for acquiring the RPM of the fan by using the signal subtracted by the subtraction unit.