Abstract: The present invention relates to a method for producing a recombinant viral vector from hairy roots of a plant, in particular from hairy roots of a plant belonging to the Brassicaceae family. The invention also relates to a transgenic plant, a hairy root culture and a recombinant viral vector obtainable by the method of the invention.

Abstract: The invention relates to a metal connecting piece (1) for connecting a shaft or handle provided with external screw thread to an implement, in which the metal connecting piece comprises a first part (2) that is at least partially provided with internal screw thread (4) for screwing in of the shaft or handle provided with external screw thread, the connecting piece being provided with a second part (3) having an internal diameter (D2) that is different from the internal diameter (D1) of the abovementioned first part (2) at least partially provided with internal screw thread (4), and said second part (3) being provided for enclosing the shaft or handle. This invention also relates to a method for producing such a metal connecting piece.

Abstract: The present invention concerns new polar compounds, complexes and compositions comprising them, wherein said compound comprises: (i) a polar headgroup spacer, (ii) at least one hydrophobic moiety, and (iii) at least one hydrophilic polymer, and wherein said polar headgroup spacer is coupled to said hydrophobic moiety and to said hydrophilic polymer.

Abstract: The invention concerns the use of a nucleic acid capable of expressing beta-interferon for the preparation of a pharmaceutical composition for the treatment of an immune disease.

Abstract: Described is the use of magnesium (Mg2+) for the preparation of a therapeutic composition for the introduction of a polynucleotide into a cell in vivo.

Abstract: Described is the use of lithium (Li+) for the preparation of a therapeutic composition for the introduction of a polynucleotide into a cell.

Abstract: Described is the use of a nuclease inhibitor or of interleukin-10 (IL-10) for the preparation of a therapeutic composition for improving transfection of a polynucleotide into a cell, and to compositions comprising a mixture of polynucleotide and nuclease inhibitor and/or interleukin-10.

Abstract: Described is a process for generating a mammalian cell line from primary mammalian cells, comprising the step of: a) pre-treating a culture of said primary mammalian cells or a suspension thereof with at least one glucocorticoid, b) optional step comprising obtaining a suspension of said pre-treated culture of step a), c) transferring into the pre-treated cells of the suspension of step a) or b) at least one nucleic acid vector which is not of retroviral origin and which is competent to immortalize said pre-treated cells and d) culturing the transferred cells of step c). Furthermore, mammalian cell lines and cells obtainable by the described process are provided as well as pharmaceutical and diagnostic compositions containing such cells.

Abstract: Described is the use of a nuclease inhibitor or of interleukin-10 (IL-10) for the preparation of a therapeutic composition for improving transfection of a polynucleotide into a cell, and to compositions comprising a mixture of polynucleotide and nuclease inhibitor and/or interleukin-10.

Abstract: Described is a human muscular cell line which is able to proliferate and generated from primary human muscular cells by a process comprising the step of: a) pre-treating a culture of said primary human muscular cells or a suspension thereof with at least one glucocorticoid, b) optional step comprising obtaining a suspension of said pre-treated culture of step a), c) transferring into the pre-treated cells of the suspension of step a) or b) at least one nucleic acid vector which is not of retroviral origin and which is competent to immortalize said pre-treated cells and d) culturing the transferred cells of step c). Furthermore, human muscular cells derived from said cell lines are provided as well as pharmaceutical and diagnostic compositions comprising the same.

Abstract: Described is the use of magnesium (Mg2+) for the preparation of a therapeutic composition for the introduction of a polynucleotide into a cell in vivo.

Abstract: The invention concerns the use of a nucleic acid capable of expressing beta-interferon for the preparation of a pharmaceutical composition for the treatment of an immune disease.

Abstract: Described is the use of magnesium (Mg2+) for the preparation of a therapeutic composition for the introduction of a polynucleotide into a cell in vivo.

Abstract: Described is the use of lithium (Li+) for the preparation of a therapeutic composition for the introduction of a polynucleotide into a cell.

Abstract: The invention relates to a composition, kit and methods of using the compositions and kits. The composition comprises a compound which disorganizes or degrades the extracellular matrix of a cell and a nucleic acid of interest. The composition or kit is contemplated for use in gene transfer and gene therapy.

Abstract: Use of eliprodil and its enantiomers for the preparation of medicines useful for the treatment of peripheral nouropathies and chronic neurodegenerative diseases of the central nervous system.