Abstract: A cell culture chamber includes: a top compartment and a bottom compartment that are separated by a semi-permeable membrane inside a supporting body; a culture solution supply flow path that supplies culture solution to an interior of the bottom compartment; a culture solution discharge flow path that discharges culture solution from the interior of the bottom compartment; a circulation flow path that is used to circulate fluid within the interior of the top compartment; and a cell culturing portion is formed inside the top compartment by a sieve structure that is formed by aperture portions through which a fluid is able to pass but through which cultured cells cannot pass, and by wall portions.

Abstract: An electrical signal measuring device that can measure electrical characteristics of cells in culture in real time and with high accuracy while maintaining them in a favorable state and an electrical signal measuring method that uses the device are disclosed. The electrical signal measuring device 1 for cells in culture includes a support body 2, a compartment in the support body, a semipermeable membrane 3, and electrodes, in which the compartment is divided by the semipermeable membrane 3 into an upper compartment 4 and a lower compartment 5; an upper electrode 61 is provided in the upper compartment 4, and a lower electrode 62 that faces the upper electrode 61 and whose facing surface 621 is in contact with the semipermeable membrane 3 is provided in the lower compartment 5; and an upper perfusion channel and a lower perfusion channel are provided in the support body 2 to separately perfuse fluid in the upper compartment 4 and the lower compartment 5 is disclosed.