Patents by Inventor Sergei Makarov
Sergei Makarov has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20100009348Abstract: This application provides methods of determining biological activities of a biological sample comprising, for example, comparing the profile of transcription factor activities in a cell contacted with the biological sample to a control profile, such as a profile of transcription factor activities in a cell not contacted with the biological sample.Type: ApplicationFiled: September 27, 2006Publication date: January 14, 2010Inventors: Sergei Romanov, Sergei Makarov
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Patent number: 7439072Abstract: There is provided a liquid droplet monitoring and measuring apparatus (1) for a liquid droplet dispensing system having a nozzle (5) with a dispensing tip (7). The apparatus (1) comprises an inner chamber (2) surrounded by an outer shield (3), preferably not in contact with each other. An RF oscillator electrically energizes the liquid through an electrode (11). The inner chamber (2) is connected through pre-amplifiers (16), band pass filters (17conditioning amplifier (20) to a signal read-out device (21). The operation of the apparatus is based on the measurement of the capacitance between the nozzle (5) and the inner chamber (2). As a droplet (15) grows on the dispensing tip (7), the capacitance between the nozzle (5) and the inner chamber (2) increases. Therefore, the signal detected and received by the signal read-out device (21) changes. This change is directly related to the volume of the droplet which can therefore be measured.Type: GrantFiled: February 27, 2004Date of Patent: October 21, 2008Assignee: Allegro Technologies LimitedInventors: Igor Shvets, Alexander Shvets, Sergei Makarov, Juergen Osing, Cecilia Franken
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Patent number: 7438858Abstract: A dispensing assembly for liquid droplets that includes a dispenser connected to a liquid carrying pipe which in turn is connected to a source of pressurized liquid. The dispenser has an elongated body member having a main bore connected to the liquid carrying pipe. At the other end, the main bore has a valve seat that is connected to a nozzle having a nozzle bore terminating in a dispensing tip. An elongated valve boss of ferromagnetic material covered with a soft polymer is mounted in the main bore and has a cross-sectional area less than that of the main bore. A separate valve boss actuating coil assembly has upper and lower coils that are separate from the main body that can be unplugged from both coils and from the liquid carrying pipe. As such, the main body can be a disposable member.Type: GrantFiled: September 30, 2003Date of Patent: October 21, 2008Assignee: The Provost Fellows and Scholars of the College of the Holy and Undivided Trinity of Queen Elizabeth near DublinInventors: Igor Shvets, Sergei Makarov, Juergen Osing
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Publication number: 20060160108Abstract: Compositions, methods and kits are provided that are useful, for example, for determining activities of multiple cis-regulatory sequences, such as promoters and enhancers, and/or multiple trans-acting factors, such as transcription factors, in a cell. In particular, in certain embodiments, compositions are provided comprising a population of polynucleotide reporter transcription units (RTUs) in which each RTU comprises a reporter sequence, a processing tag located in the reporter sequence; and a cis-regulatory element operably linked to the reporter sequence, wherein the reporter sequences between any two RTUs in the population, outside of the processing tags, are substantially identical and wherein the positions of the processing tags within the reporter sequences distinguish between any two RTUs differing, for example, in their cis-regulatory elements. The compositions, methods and kits can further be used, for example, to identify a cell type or disease state, for example, in a biological organism.Type: ApplicationFiled: November 10, 2005Publication date: July 20, 2006Inventors: Sergei Romanov, Alex Medvedev, Sergei Makarov
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Publication number: 20050223814Abstract: There is provided a liquid droplet monitoring and measuring apparatus (1) for a liquid droplet dispensing system having a nozzle (5) with a dispensing tip (7). The apparatus (1) comprises an inner chamber (2) surrounded by an outer shield (3), preferably not in contact with each other. An RF oscillator electrically energises the liquid through an electrode (11). The inner chamber (2) is connected through pre-amplifiers (16), band pass filters (17), rectifier (18), low pass filter (19) and signal conditioning amplifier (20) to a signal read-out device (21). The operation of the apparatus is based on the measurement of the capacitance between the nozzle (5) and the inner chamber (2). As a droplet (15) grows on the dispensing tip (7), the capacitance between the nozzle (5) and the inner chamber (2) increases. Therefore, the signal detected and received by the signal read-out device (21) changes. This change is directly related to the volume of the droplet which can therefore be measured.Type: ApplicationFiled: February 27, 2004Publication date: October 13, 2005Inventors: Igor Shvets, Alexander Shvets, Sergei Makarov, Juergen Osing, Cecilia Franken
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Publication number: 20040101445Abstract: A dispensing assembly for liquid droplets that includes a dispenser connected to a liquid carrying pipe which in turn is connected to a source of pressurized liquid. The dispenser has an elongated body member having a main bore connected to the liquid carrying pipe. At the other end, the main bore has a valve seat that is connected to a nozzle having a nozzle bore terminating in a dispensing tip. An elongated valve boss of ferromagnetic material covered with a soft polymer is mounted in the main bore and has a cross-sectional area less than that of the main bore. A separate valve boss actuating coil assembly has upper and lower coils that are separate from the main body that can be unplugged from both coils and from the liquid carrying pipe. As such, the main body can be a disposable member.Type: ApplicationFiled: September 30, 2003Publication date: May 27, 2004Applicant: Provost, Fellows & Scholars of College of Holy & Undivided Trinity of Queen Elizabeth Near DublinInventors: Igor Shvets, Sergei Makarov, Juergen Osing
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Patent number: 6713021Abstract: A dispensing assembly for liquid droplets that includes a dispenser connected to a liquid carrying pipe which in turn is connected to a source of pressurized liquid. The dispenser has an elongated body member having a main bore connected to the liquid carrying pipe. At the other end, the main bore has a valve seat that is connected to a nozzle having a nozzle bore terminating in a dispensing tip. A valve boss of ferromagnetic material covered with a soft polymer is mounted in the main bore and has a cross-sectional area less than that of the main bore. A separate valve boss actuating coil assembly has upper and lower coils that are separate from the main body that can be unplugged from both coils and from the liquid carrying pipe. As such, the main body can be a disposable member.Type: GrantFiled: November 13, 2000Date of Patent: March 30, 2004Assignee: The Provost, Fellows and Scholars of the College of the Holy and Undivided Trinity of Queen Elizabeth Near DublinInventors: Igor Shvets, Sergei Makarov, Juergen Osing
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Patent number: 6669909Abstract: A dispenser (1) for liquid droplets of the order of 30 &mgr;l or less in volume. The dispenser (1) comprises a metering valve body (2) having a main bore (3) connected by a pipe (4) to a pressurised liquid delivery source (not shown). The body (2) comprises a base (5) from which is mounted a nozzle (6) projecting above the base (5) to form a valve seat (7). An actuating coil assembly (11) is mounted on the exterior of the body (2) for moving a floating valve boss (15) of a ferromagnetic material. Sensing coils (20, 21) are mounted around and spaced-apart along the body (2) forming part of a valve boss detector. The valve boss detector acts as a positional movement detector so that the opening and closing of the valve can be accurately controlled and bouncing of the valve boss (15) either against an end stop (9) or on the valve seat (7) can be greatly minimized, if not prevented fully, by careful operation of the actuating coil assembly (11).Type: GrantFiled: March 26, 2001Date of Patent: December 30, 2003Assignee: Allegro Technologies LimitedInventors: Igor Shvets, Sergei Makarov, Alexander Shvets, Juergen Osing
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Publication number: 20030175163Abstract: A multi nozzle dispensing head for a dispensing assembly for liquid droplets of 10 &mgr;l or less. The dispensing assembly comprises a pressurised liquid delivery source feeding a dispenser including a metering valve and the multi nozzle dispensing head. The multi nozzle dispensing head comprises a bored primary nozzle feeding a manifold forming a split channel which in turn feeds bored secondary nozzles. The aggregate resistance to flow in all the secondary nozzle bores is greater than the resistance to flow in the split channel, generally by a factor of 10.Type: ApplicationFiled: March 18, 2002Publication date: September 18, 2003Inventors: Igor Shvets, Sergei Makarov, Jurgen Osing, David Sweeney
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Publication number: 20020177237Abstract: A dispenser (1) for liquid droplets of the order of 30 &mgr;l or less in volume. The dispenser (1) comprises a metering valve body (2) having a main bore (3) connected by a pipe (4) to a pressurised liquid delivery source (not shown). The body (2) comprises a base (5) from which is mounted a nozzle (6) projecting above the base (5) to form a valve seat (7). An actuating coil assembly (11) is mounted on the exterior of the body (2) for moving a floating valve boss (15) of a ferromagnetic material. Sensing coils (20, 21) are mounted around and spaced-apart along the body (2) forming part of a valve boss detector. The valve boss detector acts as a positional movement detector so that the opening and closing of the valve can be accurately controlled and bouncing of the valve boss (15) either against an end stop (9) or on the valve seat (7) can be greatly minimized, if not prevented fully, by careful operation of the actuating coil assembly (11).Type: ApplicationFiled: March 26, 2001Publication date: November 28, 2002Inventors: Igor Shvets, Sergei Makarov, Alexander Shvets, Juergen Osing
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Publication number: 20020168297Abstract: A conventional syringe pump (10) is used to dispense liquids of less than 5 &mgr;l in volume in a dispensing assembly (1). There is a divider barrier formed by a flexible elastomer membrane (5) clamped between an inner part (3) and an outer part (4) of a dispenser (2). The dispenser (2) has a bore divided into a system liquid reservoir (6) and a sample liquid reservoir (7). The system liquid reservoir (6) connects to the pump (10) and the sample liquid reservoir (7) communicates with a nozzle (15) having a dispensing tip (16). The membrane (5) is virtually incompressible and therefore the pump (10) can dispense accurately. Electrostatic drop detachment using an electrode (25) electrically coupled to the dispensing tip (16) and a conducting plate (19) forming a receiving electrode below a substrate (20).Type: ApplicationFiled: August 13, 2001Publication date: November 14, 2002Inventors: Igor Shvets, Sergei Makarov, Alexander Shvets, Juergen Osing