Patents by Inventor Sergei Vladimirovich Mashko

Sergei Vladimirovich Mashko has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for producing RNA
    Patent number: 11884951
    Abstract: A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.
    Type: Grant
    Filed: May 21, 2021
    Date of Patent: January 30, 2024
    Assignee: AJINOMOTO CO., INC.
    Inventors: Shuhei Hashiro, Hisashi Yasueda, Mayu Mitsuhashi, Sergei Vladimirovich Mashko, Aleksandr Aleksandrovich Krylov, Yuliya Sergeevna Lobanova
  • Method for Producing RNA
    Publication number: 20210269840
    Abstract: A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.
    Type: Application
    Filed: May 21, 2021
    Publication date: September 2, 2021
    Applicant: AJINOMOTO CO., INC.
    Inventors: Shuhei Hashiro, Hisashi Yasueda, Mayu Mitsuhashi, Sergei Vladimirovich Mashko, Aleksandr Aleksandrovich Krylov, Yuliya Sergeevna Lobanova
  • Method for producing RNA
    Patent number: 11046986
    Abstract: A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.
    Type: Grant
    Filed: September 25, 2019
    Date of Patent: June 29, 2021
    Assignee: AJINOMOTO CO., INC.
    Inventors: Shuhei Hashiro, Hisashi Yasueda, Mayu Mitsuhashi, Sergei Vladimirovich Mashko, Aleksandr Aleksandrovich Krylov, Yuliya Sergeevna Lobanova
  • Method for Producing RNA
    Publication number: 20200024629
    Abstract: A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA, which has been modified so that the activity of ribonuclease III is reduced, in a medium, to express the objective RNA and accumulate the objective RNA in cells of the bacterium, and collecting the objective RNA from the cells.
    Type: Application
    Filed: September 25, 2019
    Publication date: January 23, 2020
    Applicant: AJINOMOTO CO., INC.
    Inventors: Shuhei Hashiro, Hisashi Yasueda, Mayu Mitsuhashi, Sergei Vladimirovich Mashko, Aleksandr Aleksandrovich Krylov, Yuliya Sergeevna Lobanova
  • Method for Producing L-Amino Acids Using Bacteriua of the Enterobacteriaceae Family
    Publication number: 20140287472
    Abstract: There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.
    Type: Application
    Filed: June 11, 2014
    Publication date: September 25, 2014
    Applicant: AJINOMOTO CO., INC.
    Inventors: Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Ekaterina Alekseevna Savrasova, Valeriy Zavenovich Akhverdian, Elena Vitalievna Klyachko, Sergei Vladimirovich Mashko, Vera Georgievna Doroshenko, Larisa Gotlibovna Airikh, Tatyana Viktorovna Leonova, Mikhail Markovich Gusyatiner, Elvira Borisovna Voroshilova, Yury Ivanovich Kozlov, Yoshihiko Hara, Takuji Ueda
  • Method for producing L-amino acids using bacteria of the enterobacteriaceae family
    Patent number: 8785161
    Abstract: There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.
    Type: Grant
    Filed: May 8, 2012
    Date of Patent: July 22, 2014
    Assignee: Ajinomoto Co., Inc.
    Inventors: Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Ekaterina Alekseevna Savrasova, Valeriy Zavenovich Akhverdian, Elena Vitalievna Klyachko, Sergei Vladimirovich Mashko, Vera Georgievna Doroshenko, Larisa Gotlibovna Airikh, Tatyana Viktorovna Leonova, Mikhail Markovich Gusyatiner, Elvira Borisovna Voroshilova, Yury Ivanovich Kozlov, Yoshihiko Hara, Takuji Ueda
  • Method for producing L-amino acids using bacteria of the enterobacteriaceae family
    Patent number: 8728774
    Abstract: There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.
    Type: Grant
    Filed: February 22, 2011
    Date of Patent: May 20, 2014
    Assignee: Ajinomoto Co., Inc.
    Inventors: Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Ekaterina Alekseevna Savrasova, Valeriy Zavenovich Akhverdian, Elena Vitalievna Klyachko, Sergei Vladimirovich Mashko, Vera Georgievna Doroshenko, Larisa Gotlibovna Airikh, Tatyana Viktorovna Leonova, Mikhail Markovich Gusyatiner, Elvira Borisovna Voroshilova, Yury Ivanovich Kozlov, Yoshihiko Hara, Takuji Ueda
  • Method for constructing an operon containing translationally coupled genes
    Patent number: 8394610
    Abstract: The present invention provides a method for constructing recombinant translationally coupled operons, a method for producing useful metabolites using the bacterium containing the coupled operons, and a method for monitoring gene expression.
    Type: Grant
    Filed: February 19, 2009
    Date of Patent: March 12, 2013
    Assignee: Ajinomoto Co., Inc.
    Inventors: Andrey Yurievich Gulevich, Aleksandra Yurievna Skorokhodova, Vladimir Yurievich Ermishev, Natalya Igorevna Minaeva, Danila Vadimovich Zimenkov, Aleksandr Aleksandrovich Krylov, Irina Vladimirovna Biryukova, Sergei Vladimirovich Mashko
  • METHOD FOR PRODUCING L-AMINO ACIDS USING BACTERIA OF THE ENTEROBACTERIACEAE FAMILY
    Publication number: 20120219996
    Abstract: There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.
    Type: Application
    Filed: May 8, 2012
    Publication date: August 30, 2012
    Inventors: Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Ekaterina Alekseevna Savrasova, Valeriy Zavenovich Akhverdian, Elena Vitalievna Klyachko, Sergei Vladimirovich Mashko, Vera Georgievna Doroshenko, Larisa Gotlibovna Airikh, Tatyana Viktorovna Leonova, Mikhail Markovich Gusyatiner, Elvira Borisovna Voroshilova, Yury Ivanovich Kozlov, Yoshihiko Hara, Takuji Ueda
  • Expression control sequence
    Patent number: 7989611
    Abstract: An expression control sequence which controls expression of a target gene linked downstream of the expression control sequence depending on an intracellular concentration of an amino acid, wherein in a bacterial cell which harbors a DNA construct comprising the expression control sequence, a promoter linked upstream of the expression control sequence and the target gene linked downstream of the expression control sequence, frequency of termination in the expression control sequence, of transcription starting from the promoter is lowered by increase of an intracellular concentration of an amino acid, whereby expression of the target gene increases.
    Type: Grant
    Filed: December 3, 2004
    Date of Patent: August 2, 2011
    Assignee: Ajinomoto Co., Inc.
    Inventors: Sergei Vladimirovich Mashko, Danila Vadimovich Zimenkov
  • METHOD FOR PRODUCING L-AMINO ACIDS USING BACTERIA OF THE ENTEROBACTERIACEAE FAMILY
    Publication number: 20110143403
    Abstract: There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.
    Type: Application
    Filed: February 22, 2011
    Publication date: June 16, 2011
    Inventors: Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Ekaterina Alekseevna Savrasova, Valeriy Zavenovich Akhverdian, Elena Vitalievna Klyachko, Sergei Vladimirovich Mashko, Vera Georgievna Doroshenko, Larisa Gotlibovna Airikh, Tatyana Viktorovna Leonova, Mikhail Markovich Gusyatiner, Elvira Borisovna Voroshilova, Yury Ivanovich Kozlov, Yoshihiko Hara, Takuji Ueda
  • Method for producing a lower alkyl ester of ?-L-aspartyl-L-phenylalanine
    Patent number: 7935506
    Abstract: A method for producing lower alkyl ester of ?-L-aspartyl-L-phenylalanine by cultivating a recombinant Escherichia coli bacterium that has the ability to produce and accumulate an aromatic L-amino acid according in a culture medium to produce and accumulate L-phenylalanine in the medium and synthesizing lower alkyl ester of ?-L-aspartyl-L-phenylalanine from the aspartic acid or its derivative and the obtained L-phenylalanine.
    Type: Grant
    Filed: September 29, 2009
    Date of Patent: May 3, 2011
    Assignee: Ajinomoto Co., Inc.
    Inventors: Maria Viacheslavovna Vitushkina, Vitaliy Arkadyevich Livshits, Sergei Vladimirovich Mashko, Vera Georgievna Doroshenko, Irina Vladimirovna Biryukova, Zhanna Iosifovna Katashkina, Aleksandra Yurievna Skorokhodova, Alla Valentinovna Belareva
  • Method for producing L-amino acids using bacteria of the Enterobacteriaceae family
    Patent number: 7915018
    Abstract: There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.
    Type: Grant
    Filed: October 12, 2005
    Date of Patent: March 29, 2011
    Assignee: Ajinomoto Co., Inc.
    Inventors: Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Ekaterina Alekseevna Savrasova, Valeriy Zavenovich Akhverdian, Elena Vitalievna Klyachko, Sergei Vladimirovich Mashko, Vera Georgievna Doroshenko, Larisa Gotlibovna Airikh, Tatyana Viktorovna Leonova, Mikhail Markovich Gusyatiner, Elvira Borisovna Voroshilova, Yury Ivanovich Kozlov, Yoshihiko Hara, Takuji Ueda
  • Method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family with attenuated expression of the ydiN gene or the ydiB gene or combination thereof
    Patent number: 7820415
    Abstract: The present invention provides a method for producing an aromatic L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the ydiN gene, the ydiB gene, or both.
    Type: Grant
    Filed: February 17, 2009
    Date of Patent: October 26, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Andrey Yurievich Gulevich, Tatyana Aleksandrovna Gaydenko, Vladimir Yurievich Ermishev, Aleksandra Yurievna Skorokhodova, Irina Vladimirovna Biryukova, Sergei Vladimirovich Mashko
  • Method for producing L-amino acids using bacteria belonging to the genus escherichia
    Publication number: 20100099153
    Abstract: A method for producing lower alkyl ester of ?-L-aspartyl-L-phenylalanine by cultivating a recombinant Escherichia coli bacterium that has the ability to produce and accumulate an aromatic L-amino acid according in a culture medium to produce and accumulate L-phenylalanine in the medium and synthesizing lower alkyl ester of ?-L-aspartyl-L-phenylalanine from the aspartic acid or its derivative and the obtained L-phenylalanine.
    Type: Application
    Filed: September 29, 2009
    Publication date: April 22, 2010
    Applicant: Ajinomoto Co., Inc.
    Inventors: Maria Viacheslavovna Vitushkina, Vitaliy Arkadyevich Livshits, Sergei Vladimirovich Mashko, Vera Georgievna Doroshenko, Irina Vladimirovna Biryukova, Zhanna Iosifovna Katashkina, Aleksandra Yurievna Skorokhodova, Alla Valentinovna Belareva
  • bacteria transformed with the yddG gene to enhance L-amino acid producing activity
    Patent number: 7666655
    Abstract: The present invention relates to a method for producing L-amino acid, such as L-phenylalanine and L-tryptophan, is provided using bacterium belonging to the genus Escherichia wherein the L-amino acid productivity of said bacterium is enhanced by enhancing an activity of protein encoded by the yddG gene from Escherichia coli, wherein said protein has an activity to make said bacterium resistant to L-phenylalanine, a phenylalanine analogue, or a tryptophan analogue. The present invention further relates to a method for producing lower alkyl ester of ?-L-aspartyl-L-phenylalanine.
    Type: Grant
    Filed: November 25, 2002
    Date of Patent: February 23, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Maria Viacheslavovna Vitushkina, Vitaliy Arkadyevich Livshits, Sergei Vladimirovich Mashko, Vera Georgievna Doroshenko, Irina Vladimirovna Biryukova, Zhanna Iosifovna Katashkina, Aleksandra Yurievna Skorokhodova, Alla Valentinovna Belareva
  • Method for producing an L-amino acid using a bacterium with an optimized level of gene expression
    Patent number: 7604979
    Abstract: A method is provided for obtaining an L-amino acid or nucleic acid-producing bacterium belonging to the genus Escherichia with an optimized level of expression of the gene which influences the distribution of carbon flow, such as the sucAB genes, comprising introducing into the chromosome of the bacterium a set of in vitro constructed DNA fragments which contain regulatory elements for gene expression instead of the native elements of the regulatory region of the gene, and selecting the colonies with increased L-amino acid productivity. Also, a method is provided for producing an L-amino acid, such as L-glutamic acid, L-proline, L-arginine, L-glutamine, L-leucine, using the bacterium with an optimized level of expression of the sucAB gene.
    Type: Grant
    Filed: September 12, 2005
    Date of Patent: October 20, 2009
    Assignee: Ajinomoto Co., Inc.
    Inventors: Joanna Yosifovna Katashkina, Maria Grigorievna Lunts, Vera Georgievna Doroshenko, Svetlana Aleksandrovna Fomina, Aleksandra Yurievna Skorokhodova, Lirina Valerievna Ivanovskaya, Sergei Vladimirovich Mashko
  • METHOD FOR CONSTRUCTING AN OPERON CONTAINING TRANSLATIONALLY COUPLED GENES
    Publication number: 20090226919
    Abstract: The present invention provides a method for constructing recombinant translationally coupled operons, a method for producing useful metabolites using the bacterium containing the coupled operons, and a method for monitoring gene expression.
    Type: Application
    Filed: February 19, 2009
    Publication date: September 10, 2009
    Inventors: Andrey Yurievich Gulevich, Aleksandra Yurievna Skorokhodova, Vladimir Yurievich Ermishev, Natalya Igorevna Minaeva, Danila Vadimovich Zimenkov, Aleksandr Aleksandrovich Krylov, Irina Vladimirovna Biryukova, Sergei Vladimirovich Mashko
  • METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY WITH ATTENUATED EXPRESSION OF THE ydiN GENE OR THE ydiB GENE OR COMBINATION THEREOF
    Publication number: 20090191601
    Abstract: The present invention provides a method for producing an aromatic L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the ydiN gene, the ydiB gene, or both.
    Type: Application
    Filed: February 17, 2009
    Publication date: July 30, 2009
    Inventors: Andrey Yurievich Gulevich, Tatyana Aleksandrovna Gaydenko, Vladimir Yurievich Ermishev, Aleksandra Yurievna Skorokhodova, Irina Vladimirovna Biryukova, Sergei Vladimirovich Mashko
  • Method of producing amino acids using transformed with sucrose PTS genes
    Patent number: 7179623
    Abstract: Amino acids such as threonine, homoserine, isoleucine, lysine, valine and tryptophan are produced using a bacterium belonging to the genus Escherichia which has been constructed from a sucrose non-assimilative strain belonging to the genus Escherichia and which harbors sucrose non-PTS or PTS genes and has an ability to produce the amino acid.
    Type: Grant
    Filed: August 16, 2005
    Date of Patent: February 20, 2007
    Assignee: Ajinomoto Co., Inc.
    Inventors: Vitaliy Arkadyevich Livshits, Vera Georgievna Doroshenko, Sergei Vladimirovich Mashko, Valery Zavenovich Akhverdian, Yury Ivanovich Kozlov