Abstract: The invention generally relates to methods of using compositions that include sets of magnetic particles, members of each set being conjugated to an antibody specific for a pathogen, and magnets to isolate a pathogen from a body fluid sample.

Abstract: The invention generally relates to compositions that include sets of magnetic particles, members of each set being conjugated to an antibody specific for a pathogen.

Abstract: The invention generally relates to using magnetic particles and alternating magnet fields to separate a target analyte from a sample. In certain embodiments, methods of the invention involve contacting a sample with magnetic particles including first moieties specific for a target analyte, thereby forming target/particle complexes in the sample, flowing the sample through a channel including second moieties attached to at least one surface of the channel, applying alternating magnetic fields to the flowing sample to result in target/particle complexes being brought into proximity of the surface to bind the second moieties and unbound particles remaining free in the sample, binding the target/particle complexes to the second moieties, and washing away unbound particles and unbound analytes of the sample.

Abstract: The invention generally relates to methods of using compositions that include sets of magnetic particles, members of each set being conjugated to an antibody specific for a pathogen, and magnets to isolate a pathogen from a body fluid sample.

Abstract: The invention generally relates to methods of using compositions that include sets of magnetic particles, members of each set being conjugated to an antibody specific for a pathogen, and magnets to isolate a pathogen from a body fluid sample.

Abstract: Separation media comprising a support and crosslinked whole cells fixed to the support. The separation media are useful in affinity columns for the separation of the antibodies from a solution, particularly for the separation of polyclonal antibodies that have been raised against the same type of whole cells. In an embodiment, the whole cells are whole bacterial cells.

Abstract: Methods for degrading contaminant nucleic acid. The methods use combinations of metal ions and peroxide ions to produce a variety of oxidative species that degrade nucleic acid. Methods of the invention are useful for decontaminating laboratory equipment or solutions. After the equipment or solutions have been decontaminated, the metal ion and peroxide ion solution can be deactivated by raising the temperature to dissociate the peroxide or by binding the metal ions, e.g., with a chelating agent.

Abstract: The present invention generally relates to methods of generating antibodies against a species of pathogen that involve identifying the pathogen that is most genetically representative of member of pathogen species and using the identified pathogen to generate an antibody.

Abstract: Methods of the invention generally involve using magnetic particles to isolate low levels of pathogens from a samples and identifying genes expressed by those pathogen. In one aspect, the method includes obtaining a sample comprising a pathogen, forming magnetic particle/target complexes, separating the magnetic particle/target complexes using magnetic fields, and determining an expression profile of a nucleic acid derived from the target.

Abstract: Viral replicon selected nucleic acid expression libraries are useful for analyzing multiple antigens associated with a parasite, pathogen or neoplasia or for preparing immunogenic compositions for generating immune responses specific for the parasite, pathogen or neoplasia. Alphavirus replicon particles representative of the nucleic acid expression library are preferred. The nucleic acid library can be a random library, or it can be prepared after a selection step, for example, by differential hybridization prior to cloning into the replicon vector.

Abstract: The invention generally relates to methods of using compositions that include sets of magnetic particles, members of each set being conjugated to an antibody specific for a pathogen, and magnets to isolate a pathogen from a body fluid sample.

Abstract: The invention generally relates to using magnetic particles and magnets to isolate a target analyte from a sample. In certain embodiments, methods of the invention involve introducing to a sample at least one set of magnetic particles having at least one type of lectin in order to create a mixture, incubating the mixture such that the lectin binds to a carbohydrate on a surface of a target in the sample to thereby form target/particle complexes, in which the incubating is performed in the presence of a buffer that influences binding, and applying a magnetic field to separate target/particle complexes from remaining components of the sample, thereby isolating target/magnetic particle complexes.

Abstract: The invention generally relates to using magnetic particles and magnets to isolate a target analyte from a body fluid sample. In certain embodiments, methods of the invention involve introducing magnetic particles including a target-specific binding moiety to a body fluid sample in order to create a mixture, incubating the mixture to allow the particles to bind to a target, applying a magnetic field to capture target/magnetic particle complexes on a surface, and washing with a wash solution that reduces particle aggregation, thereby isolating target/magnetic particle complexes.

Abstract: The invention generally relates to methods of using compositions that include sets of magnetic particles, members of each set being conjugated to an antibody specific for a pathogen, and magnets to isolate a pathogen from a body fluid sample.

Abstract: The invention generally relates to compositions that include sets of magnetic particles, members of each set being conjugated to an antibody specific for a pathogen.

Abstract: The invention generally relates to compositions that include magnetic particles bound to pathogens in a body fluid.

Abstract: The invention generally relates to compositions and methods for isolating mid-log phase bacteria. In certain embodiments, the invention provides a magnetic particle conjugated to an antibody that includes an epitope specific for mid-log phase bacteria.

Abstract: The invention generally relates to using magnetic particles and alternating magnet fields to separate a target analyte from a sample. In certain embodiments, methods of the invention involve contacting a sample with magnetic particles including first moieties specific for a target analyte, thereby forming target/particle complexes in the sample, flowing the sample through a channel including second moieties attached to at least one surface of the channel, applying alternating magnetic fields to the flowing sample to result in target/particle complexes being brought into proximity of the surface to bind the second moieties and unbound particles remaining free in the sample, binding the target/particle complexes to the second moieties, and washing away unbound particles and unbound analytes of the sample.

Abstract: The invention generally relates to conducting an assay on a sample that isolates a pathogen from the sample and allows for analysis of the pathogen with minimal (i.e., at most 24 hrs of culturing) or no culturing of the pathogen. In certain embodiments, the invention provides methods for identifying a pathogen from a sample that involve obtaining a sample including a pathogen, conducting an assay that isolates the pathogen from the sample, culturing the isolated pathogen for at most about 24 hrs, and analyzing the pathogen.

Abstract: The invention generally relates to conducting an assay on a sample that isolates a bacterium from the sample, in which the assay isolates as low as about 1 CFU/ml of bacteria in the sample.